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Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

Postal Subscription Code 80-906

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Front. Agr. Sci. Eng.    2015, Vol. 2 Issue (3) : 223-229    https://doi.org/10.15302/J-FASE-2015065
RESEARCH ARTICLE
Characterization of NDM-1-producing carbapenemase in Acinetobacter spp. and E. coli isolates from diseased pigs
Rongmin ZHANG,Yang WANG,Zhihai LIU,Jiyun LI,Wenjuan YIN,Lei LEI,Congming WU,Jianzhong SHEN()
Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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Abstract

In recent years, the mobile metallo-β-lactamase (MBL) genes have been found to correspond to one of the most important resistance characters identified in Gram-negative bacteria, severely affecting clinical chemotherapy and threatening public health. The prevalence of mobile MBL genes and their flanking regions in Gram-negative bacteria from diseased pigs in China was investigated. A total of 334 lung samples from diseased pigs were screened for Gram-negative bacteria classified as non-susceptible to meropenem (MIC≥4 mg·L−1). Six isolates, including three Escherichia coli, two Acinetobacter baumanii and one A. calcoaeticus, exhibited MBL production and carried the blaNDM-1 gene. S1-PFGE and Southern blot analysis showed that the blaNDM-1 gene was located on the chromosome of one A. baumanii isolate and on plasmids of various sizes in the other five isolates. MIC testing using broth microdilution revealed that all blaNDM-1-carrying isolates and some of their transconjugants exhibited resistance to almost all β-lactams tested. Whole genome sequencing revealed that the flanking region of the blaNDM-1 gene from all porcine isolates had high levels of similarity with the corresponding regions in human isolates. One porcine E. coli isolate carrying blaNDM-1 was typed as ST48, a common sequence type in human E. coli isolates. These results suggest the possibility of human-to-food animal transfer of blaNDM-1-producing E. coli, highlighting the need for surveillance of carbapenemase producers among bacteria from food animals. In addition, the prudent use of antimicrobial agents to decrease the opportunities for co-selection of carbapenemase genes in food animals is also urgently needed.
Keywords carbapenemase      NDM-1      ISAba125      Enterobacteriaceae      food safety     
Corresponding Author(s): Jianzhong SHEN   
Just Accepted Date: 28 July 2015   Online First Date: 17 August 2015    Issue Date: 10 November 2015
 Cite this article:   
Rongmin ZHANG,Yang WANG,Zhihai LIU, et al. Characterization of NDM-1-producing carbapenemase in Acinetobacter spp. and E. coli isolates from diseased pigs[J]. Front. Agr. Sci. Eng. , 2015, 2(3): 223-229.
 URL:  
https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2015065
https://academic.hep.com.cn/fase/EN/Y2015/V2/I3/223
Isolate Species PFGE type MLST type MIC/(mg·L−1) Location of blaNDM-1
IMP MERO CTZ CAZ AZT CIP GEN COL FFC TET
FSAB08 A. baumanii 1 – 512 512 >1024 256 >512 2 64 128 8 32 C
FSAB62 A. baumanii 2 – 256 256 1024 >1024 64 128 0.125 4 256 512 P/pAB62(~47 kb)
FSABC15 A. calcoaceticus – – 256 512 1024 >1024 128 256 128 4 512 >512 P/pABC15 (~47 kb)
FSEC38 E. coli a 5084 64 32 1024 >1024 512 2 128 8 512 256 P/pEC38 (~50 kb)
FSEC39 E. coli b 5069 128 128 512 >1024 4 8 >512 2 32 4 P/pEC39 (~70 kb)
EC600-39 E. coli – – 32 32 128 1024 0.25 0.125 512 0.5 4 4 P/pEC39 (~70 kb)
FSEC69 E. coli c 48 128 256 >1024 >1024 16 128 256 4 512 256 P/pEC69 (~200 kb)
EC600-69 E. coli – – 64 64 256 >1024 0.125 0.25 64 0.5 512 64 P/pEC69 (~200 kb)
EC600 E. coli – – 0.25 0.03 2 0.5 0.125 0.015 1 0.25 4 2 –
Tab.1  Antimicrobial susceptibility profiles of Acinetobacter. baumannii FSAB08 and FSAB62, A. calcoaceticus FSABC15, E. coli FSEC38, FSEC39, FSEC69, transconjugants EC600-39 and EC600-69, and recipient strains EC600
Fig.1  Localization of blaNDM-1 in NDM-1-producing isolates by S1-PFGE (a) and Southern blot hybridization with blaNDM-1 probe (b). Lane M, low-range pulsed-field gel marker (New England BioLabs, Beverly, MA). Lane 17–3, E. coli isolates FSEC38, FSEC39 and FSEC69; Lane 4, Acinetobacter. calcoaceticus isolate FSABC15; Lane 5–6, A. baumanii isolates FSAB62 and FSAB08.
Fig.2  Genetic environment of blaNDM-1 in E. coli and Acinetobacter spp. isolates of pig origin, and its structural comparison with the corresponding genetic regions in plasmids pAL-1, pNDM-SX04, and pGUE-NDM. The arrows indicate the positions and directions of transcription of the genes. Different genes are indicated by different types of shading. Regions of≥99.8% homology are marked by gray shading
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