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Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

Postal Subscription Code 80-906

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Front. Agr. Sci. Eng.    2015, Vol. 2 Issue (3) : 260-265    https://doi.org/10.15302/J-FASE-2015070
RESEARCH ARTICLE
Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana
Jing WANG1,2,Chen CHENG1,2,Yanru ZHOU1,2,Yong YANG2,Qiong MEI3,2,Junmin LI2,Ye CHENG2,Chengqi YAN2,*(),Jianping CHEN2,*()
1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China
2. State Key Laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of the Ministry of Agriculture and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
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Abstract

Y73 is a progeny of asymmetric somatic hybridization between Oryza sativa cv. Dalixiang and the wild rice species Oryza meyeriana. Inoculation with a range of strains of Xanthomonas oryzae pv. oryzae showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F2 population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F2 population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.
Keywords Oryza meyeriana      bacterial blight resistance gene      genetic population      linkage molecular marker     
Corresponding Author(s): Chengqi YAN,Jianping CHEN   
Just Accepted Date: 09 October 2015   Online First Date: 03 November 2015    Issue Date: 10 November 2015
 Cite this article:   
Jing WANG,Chen CHENG,Yanru ZHOU, et al. Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana[J]. Front. Agr. Sci. Eng. , 2015, 2(3): 260-265.
 URL:  
https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2015070
https://academic.hep.com.cn/fase/EN/Y2015/V2/I3/260
Fig.1  Bacterial blight lesion sizes (% leaf length) of Oryza meyeriana, Dalixiang, Y73 and IR24 at 21 days after inoculation with different Xanthomonas oryzae pv. oryzae test strains. 1 to 10 are ten different X. oryzae pv. oryzae strains of PXO86, PXO79, PXO71, PXO112, PXO99, PXO280, PXO145, PXO87, PXO124 and ZHE173, respectively. Bars show the SE of treatment means.
Fig.2  Locations of the linkage regions (shown in black) on the genetic maps of rice chromosomes 1, 3 and 5. The left sides of the chromosomes show the genetic distance (cmol) between adjacent markers, and the marker names are indicated on the right sides of the chromosomes.
Markers Chromosome Location/cmol Y73 Hybrid IR24 χ2
(a) Resistant
RM5919 1 101.0 32 8 10 54.1
RM5461 1 112.7 33 6 11 60.3
RM128 1 134.8 35 2 13 74.1
RM1361 1 155.2 32 9 9 53.1
RM5310 1 164.1 32 13 5 50.9
R03D143 3 143.0 24 17 9 22.8
R03D146 3 146.4 26 17 7 28.2
R03D158 3 158.5 33 4 13 63.6
R03D159 3 159.0 33 9 8 56.9
RM55 3 168.2 25 16 9 25.8
RM7081 5 103.6 34 9 7 61.1
R05D104 5 103.9 37 8 5 75.7
RM3348 5 104.4 39 4 7 88.5
RM6972 5 104.7 39 6 5 87.1
RM3616 5 107.4 36 7 7 71.4
(b) Susceptible
RM1361 1 155.2 9 5 36 73.3
R03D159 3 159.0 8 10 32 52.2
RM6972 5 104.7 12 5 33 61.8
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