<i>RR1</i> and <i>RR2</i> gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

doi:10.15302/J-FASE-2016088

Front. Agr. Sci. Eng.

2016, Vol. 3

Issue (1) : 81-86 https://doi.org/10.15302/J-FASE-2016088

RESEARCH ARTICLE

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Shijun YAN¹,He YAN¹,Chaolin ZHANG¹,Tongyan WANG¹,Qingyuan YANG¹,Zhe SUN¹,Yan XIAO¹,Feifei TAN¹,Xiangdong LI^1,^*(

),Kegong TIAN^1,^2,^*(

)

1. National Research Center for Veterinary Medicine, Luoyang 471003, China
2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

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Abstract

As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/gE/gI triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

Keywords pseudorabies virus RR1 and RR2 ribonucleotide reductase vaccine candidate

Corresponding Author(s): Xiangdong LI,Kegong TIAN

Just Accepted Date: 16 March 2016 Online First Date: 31 March 2016 Issue Date: 07 April 2016

Cite this article:

Shijun YAN,He YAN,Chaolin ZHANG, et al. RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host[J]. Front. Agr. Sci. Eng. , 2016, 3(1): 81-86.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2016088
https://academic.hep.com.cn/fase/EN/Y2016/V3/I1/81

Fig.1 Replication kinetics (a) and plaque size (b) of vPRVTK^–/gE^–/gI^–/RR^–. The virus titer at each time points and plaque size were averaged by triple samples.

Fig.2 PRV gB ELISA Ab responses (a, b) and PRV-specific virus neutralizing antibodies (c). The cutoff S/N values for the gB ELISA are: negative as greater than 0.7, positive as less than or equal to 0.6, and suspect as greater than 0.6 and less than or equal to 0.7. *, P<0.05.

Fig.3 Body temperature (a) and clinical symptom scores (b) of pigs after viral challenge. *, P<0.05.

Fig.4 Survival rate (a) and daily bodyweight gain for 4 dpc (b) of pigs after viral challenge. *,P<0.05.

Fig.5 Histopathological (a–h) and immunochemistry (i–p) results of vPRVTK^–/gE^–/gI^– (a–d, i–l) and vPRVTK^–/gE^–/gI^–/RR^– (e–h, m–p). (a–d) Tonsil, lung, brain, and cerebellum samples from the pigs in vPRVTK^–/gE^–/gI^– group. No obvious histopathological changes were observed; (e) tonsil. Tonsillar lymphoid tissue necrosis and formation of a big necrotic foci; (f) lung. Massive vascular congestion and hemorrhage; (g) brain. Nonsuppurative meningoencephalitis; (h) cerebellum. Purkinje cell degeneration and necrosis; (i–l) negative immunochemistry staining of corresponding tonsil, lung, brain, and cerebellum samples in vPRVTK^–/gE^–/gI^–group; (m–p) positive immunochemistry staining of corresponding tonsil, lung, brain, and cerebellum samples in vPRVTK^–/gE^–/gI^–/RR^–group. Original magnification 200×.

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