A comparison of the biological activities of ethyl acetate fractions from the stems and leaves of <i>Penthorum chinense</i> Pursh

doi:10.15302/J-FASE-2019271

Front. Agr. Sci. Eng.

2020, Vol. 7

Issue (4) : 505-512 https://doi.org/10.15302/J-FASE-2019271

RESEARCH ARTICLE

A comparison of the biological activities of ethyl acetate fractions from the stems and leaves of Penthorum chinense Pursh

Zhaolei WANG¹, Kai JIANG¹, Qinchao DING¹, Shujun LIU², Xiaobing DOU¹, Bin DING¹(

)

¹. College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
². Beijing Stomatological Hospital/School of Stomatology, Capital Medical University, Beijing 100050, China

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Abstract

Penthorum chinense Pursh (PCP) is a popular traditional medicinal plant in China, widely used for the treatment of a variety of liver diseases. Although it has been long recognized that the main active elements of PCP are contained in ethyl acetate fraction (EAF), little is known so far in terms of the relative effectiveness of EAF derived from the stems versus leaves of this plant. In the current study, we prepared EAF by reflux extraction and sequential extraction from the stems (SEAF) and leaves (LEAF) of PCP and tested their hepatoprotective efficacies. The extract rates and flavonoid contents of LEAF were higher than those of SEAF. EAFs (>50 μg·mL⁻¹) prevented lipid accumulation in cells and protected against lipotoxicity injury when the concentration exceeded 25 μg·mL⁻¹. More than 95% free radicals released by 2,2-diphenyl-1-picrylhydrazyl (DPPH) were eliminated by 25 μg·mL⁻¹ SEAF and 50 μg·mL⁻¹ LEAF, respectively. Further, EAFs (25 μg·mL⁻¹) also showed protective antioxidant effects, with the activity of LEAF being significantly higher than that of SEAF. EAFs (10 mg·mL⁻¹) also showed similar unspecific bacteriostatic activity. In comparison with SEAF, LEAF contained more flavonoids and had a higher anti-oxidation capability and for these reasons we suggest it should be better for clinical use.

Keywords antibacterial anti-oxidation lipid accumulation lipotoxicity Penthorum chinense Pursh

Corresponding Author(s): Bin DING

Just Accepted Date: 31 May 2019 Online First Date: 28 June 2019 Issue Date: 06 November 2020

Cite this article:

Zhaolei WANG,Kai JIANG,Qinchao DING, et al. A comparison of the biological activities of ethyl acetate fractions from the stems and leaves of Penthorum chinense Pursh[J]. Front. Agr. Sci. Eng. , 2020, 7(4): 505-512.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2019271
https://academic.hep.com.cn/fase/EN/Y2020/V7/I4/505

Fig.1 Cell protective efficiency of SEAF and LEAF against OA induced lipid accumulation. (a) The images of BODIPY stained HepG2 cells; (b) the relative TG content. Control (C) and model (M) groups are untreated and 0.5 mmol·L⁻¹ OA treated HepG2 cell groups, respectively. The others are sample groups that 12.5, 25, 50, 100 μg·mL⁻¹ SEAF and LEAF protected HepG2 cell groups, against 0.5 mmol·L⁻¹ OA treatment. # indicates significant difference between the C and M groups; * indicates significant difference between the sample and M groups; NS indicates nonsignificant difference between the same concentrations of SEAF and LEAF (P<0.05).

Fig.2 Lipotoxicity attenuation efficiency of SEAF and LEAF against PA induced cell injury, measured as the relative amounts of released LDH. Control (C) and model (M) groups are untreated and 0.5 mmol·L⁻¹ PA treated HepG2 cell groups, respectively. The others are sample groups that 12.5, 25, 50, 100 μg·mL⁻¹ SEAF and LEAF protected HepG2 cell groups, against 0.5 mmol·L⁻¹ PA treatment. # indicates significant difference between the C group and M group; * indicates significant difference between the sample group and M group; NS indicates nonsignificant difference between the same concentrations of SEAF and LEAF (P<0.05).

Tab.1 Relative amount of released LDH in PA treated HepG2 assays protected by different concentrations of EAFs (mg·mL⁻¹)

Fig.3 DPPH free radical clearance activities of SEAF and LEAF. * indicates significant difference between SEAF and LEAF (P<0.05).

Fig.4 Cell protective efficiency of SEAF and LEAF against H₂O₂ oxidative damage. (a)The images of Hoechst stained HepG2 cells; (b) the relative amounts of released LDH. Control (C) and model (M) groups are untreated and 0.4 mmol·L⁻¹ H₂O₂ treated HepG2 cells, respectively. The others are sample groups that 12.5, 25, 50, 100 μg·mL⁻¹ SEAF and LEAF protected HepG2 cell groups, against 0.4 mmol·L⁻¹ H₂O₂ treatment. # indicates significant differences between the M and the C. * indicates significant differences between the EAF sample groups and M group. NS and ** indicate nonsignificant or significant differences, respectively, between the same concentrations of SEAF and LEAF (P<0.05).

Tab.2 Relative amount of released LDH in H₂O₂ treated HepG2 assays protected by different concentrations of EAFs (mg·mL⁻¹)

Tab.3 Inhibitory zone diameter (mean ± SD) of SEAF and LEAF against different bacterial strains

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