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Frontiers of Chemical Science and Engineering

ISSN 2095-0179

ISSN 2095-0187(Online)

CN 11-5981/TQ

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Front Chem Sci Eng    2012, Vol. 6 Issue (4) : 453-460    https://doi.org/10.1007/s11705-012-1210-8
RESEARCH ARTICLE
Repeated batch fermentation with water recycling and cell separation for microbial lipid production
Yumei WANG, Wei LIU, Jie BAO()
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
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Abstract

Large waste water disposal was the major problem in microbial lipid fermentation because of low yield of lipid. In this study, the repeated batch fermentation was investigated for reducing waste water generated in the lipid fermentation of an oleaginous yeast Trichosporon cutaneum CX1 strain. The waste fermentation broth was recycled in the next batch operation after the cells were separated using two different methods, centrifugation and flocculation. Two different sugar substrates, glucose and inulin, were applied to the proposed operation. The result showed that at least 70% of the waste water was reduced, while lipid production maintained satisfactory in the initial four cycles. Furthermore, it is suggested that T. cutaneum CX1 cells might produce certain naturally occurring inulin hydrolyzing enzyme(s) for obtaining fructose and glucose from inulin directly. Our method provided a practical option for reducing the waste water generated from microbial lipid fermentation.
Keywords batch fermentation      microbial lipid      Trichosporon cutaneum CX1      flocculation      waste water recycle     
Corresponding Author(s): BAO Jie,Email:jbao@ecust.edu.cn   
Issue Date: 05 December 2012
 Cite this article:   
Yumei WANG,Wei LIU,Jie BAO. Repeated batch fermentation with water recycling and cell separation for microbial lipid production[J]. Front Chem Sci Eng, 2012, 6(4): 453-460.
 URL:  
https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1210-8
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I4/453
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Fig.1  Repeated batch fermentation of glucose for lipid production with water recycling and cell separation. (a) With cell separation using centrifugation and water recycling. Cells were separated and the fermentation liquid was recycled. The same fermentation nutrition was added to the recycled water. (b) Without cell separation and water recycling
Seeds culture timeAfter 20 hAfter 54 h
Inoculation ratio /(v·v-1)10%5%10%20%
DCM /(g·L-1)8.756.366.336.89
Lipid content /(g per 100 g)19.230.730.129.1
Tab.1  Effect of the culture time of seeds on lipid accumulation
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.2  Repeated batch fermentation of inulin for lipid production with cell separation by centrifugation and water recycling. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
Fig.3  Time course of glucose and fructose using inulin for lipid fermentation. (a) With cell separation by centrifugation and water recycling. (b) Without cell separation and water recycling
ParametersCell separation methodGlucoseInulin
Lipid production /(g·L-1)Control1.461.68
Centrifugation1.701.53
Flocculation1.301.34
Lipid content /(g·g-1)Control0.300.28
Centrifugation0.320.32
Flocculation0.280.28
Lipid yield YL/S /(g·g-1)Control0.100.11
Centrifugation0.110.11
Flocculation0.100.07
Water consumed /LControl5.005.00
Centrifugation1.201.20
Flocculation1.361.36
Tab.2  Fermentation parameters under different modes*
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
Fig.4  Time course of lipid fermentation with cell separation by flocculation. (a) With cell separation by flocculation and water recycling when glucose was used. (b) With cell separation by flocculation and water recycling when inulin was used. (c) The time course of glucose and fructose concentrations in the fermentation process of the substrate of inulin
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