Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric detection of epinephrine

doi:10.1007/s11705-020-1940-y

Front. Chem. Sci. Eng.

2021, Vol. 15

Issue (2) : 310-318 https://doi.org/10.1007/s11705-020-1940-y

RESEARCH ARTICLE

Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric detection of epinephrine

Miao Guan¹, Mengfan Wang^1,³(

), Wei Qi^1,^2,³, Rongxin Su^1,^2,³, Zhimin He¹

¹. School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300350, China
². The Co-Innovation Centre of Chemistry and Chemical Engineering of Tianjin, Tianjin 300350, China
³. Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin 300350, China

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Abstract

Recently, many efforts have been dedicated to creating enzyme-mimicking catalysts to replace natural enzymes in practical fields. Inspired by the pathological biomineralization behaviour of L-cystine, in this study, we constructed a laccase-like catalyst through the co-assembly of L-cystine with Cu ions. Structural analysis revealed that the formed catalytic Cu-cystine nanoleaves (Cu-Cys NLs) possess a Cu(I)-Cu(II) electron transfer system similar to that in natural laccase. Reaction kinetic studies demonstrated that the catalyst follows the typical Michaelis-Menten model. Compared with natural laccase, the Cu-Cys NLs exhibit superior stability during long-term incubation under extreme pH, high-temperature or high-salt conditions. Remarkably, the Cu-Cys NLs could be easily recovered and still maintained 76% of their activity after 8 cycles. Finally, this laccase mimic was employed to develop a colorimetric method for epinephrine detection, which achieved a wider linear range (9–455 μmol·L⁻¹) and lower limit of detection (2.7 μmol·L⁻¹). The Cu-Cys NLs also displayed excellent specificity and sensitivity towards epinephrine in a test based on urine samples.

Keywords biomineralization laccase L-cystine colorimetric detection enzyme mimic

Corresponding Author(s): Mengfan Wang

Just Accepted Date: 14 May 2020 Online First Date: 13 July 2020 Issue Date: 10 March 2021

Cite this article:

Miao Guan,Mengfan Wang,Wei Qi, et al. Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric detection of epinephrine[J]. Front. Chem. Sci. Eng., 2021, 15(2): 310-318.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-020-1940-y
https://academic.hep.com.cn/fcse/EN/Y2021/V15/I2/310

Fig.1 (a) The formation of Cu-Cys NLs through the biomineralization of L-cystine with Cu ions; (b) SEM, (c and d) TEM and SAED analysis of Cu-Cys NLs.

Fig.2 (a) XRD, (b) FTIR, (c) full scan XPS and (d) Cu 2p XPS analyses of Cu-Cys NLs.

Fig.3 (a) Laccase-like catalytic reaction using 2,4-DP and 4-AP as the substrates; (b) UV-vis spectrum of the reaction solution catalysed by Cu-Cys NLs; (c) steady-state kinetics, (d) optimum catalytic temperature and (e) pH of Cu-Cys NLs.

Fig.4 Comparison of the long-term stability of Cu-Cys NLs and laccase under different (a) temperatures, (b) pH and (c) NaCl concentrations; (d) reusability of Cu-Cys NLs.

Fig.5 (a) Epinephrine and its oxidized product catalysed by Cu-Cys NLs; (b) linear relationship between OD_{485 nm} and the concentration of epinephrine; (c) interference test for epinephrine detection.

Tab.1 Epinephrine detection in real samples

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