Based on the concept of learning stickiness, this study constructed a model of influencing factors among the elements of online learning content, interaction, incentive, satisfaction, and learning stickiness from Comprehensive Learning Theory. Structural equation modeling was used to analyze the interaction and influence effects among the factors. It is found that the content, interaction, and incentive in Comprehensive Learning Theory had a significant positive impact on learning stickiness from the total effect analysis. From the direct effect analysis, the influence of content and interaction on learning stickiness was not substantial, but the influence of incentive and satisfaction on learning stickiness was significant. From the perspective of mediation effect analysis, incentive and satisfaction were critical mediating variables for the influence of content and interaction on learning stickiness. This study put forward suggestions and strategies for online teaching, providing a reference for teachers to carry out online education.