Calcification response of <i>Pleurochrysis carterae</i> to iron concentrations in batch incubations: implication for the marine biogeochemical cycle

doi:10.1007/s11707-016-0629-5

Front. Earth Sci.

2017, Vol. 11

Issue (4) : 682-688 https://doi.org/10.1007/s11707-016-0629-5

RESEARCH ARTICLE

Calcification response of Pleurochrysis carterae to iron concentrations in batch incubations: implication for the marine biogeochemical cycle

Xiang ZOU^1,^2,³, Shiyong SUN^1,^2,³(

), Sen LIN¹, Kexuan SHEN^1,³, Faqin DONG¹, Daoyong TAN¹, Xiaoqin NIE¹, Mingxue LIU¹, Jie WEI¹

¹. School of Environment and Resources, Key Laboratory of Solid Waste Treatment and Resource Recycle, Southwest University of Science and Technology, Mianyang 621010, China
². State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, China
³. Low-cost Wastewater Treatment Technology International Sci-Tech Cooperation Base of Sichuan Province, Mianyang 621010, China

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Abstract

Calcified coccolithophores, a diverse and widely distributed group of marine microalgae, produce biogenic calcite in the form of coccoliths located on the cell surface. Using batch incubations of the coccolithophoridPleurochrysis carterae, we investigated the responses of this calcification process to iron concentrations by changing the iron supply in the initial culture media from a normal concentration to 1 ppm (parts per million), 5 ppm, and 10 ppm. Time-dependent measurements of cell population, production of inorganic carbon (coccoliths), and organic carbon (organic cellular components) showed that elevated iron supply in the growth medium ofP. carterae stimulates carbon sequestration by increasing growth along enhanced photosynthetic activity and calcification. In addition, the acquired time-dependent UV-Vis and FT-IR spectra revealed that iron fertilization-enhanced coccolith calcification is accompanied by a crystalline phase transition from calcite to aragonite or amorphous phase. Our results suggest that iron concentration has a significant influence on the marine carbon cycle of coccolithophores.

Keywords calcification coccolithophores iron fertilization Pleurochrysis carterae

Corresponding Author(s): Shiyong SUN

Just Accepted Date: 19 December 2016 Online First Date: 17 March 2017 Issue Date: 10 November 2017

Cite this article:

Xiang ZOU,Shiyong SUN,Sen LIN, et al. Calcification response of Pleurochrysis carterae to iron concentrations in batch incubations: implication for the marine biogeochemical cycle[J]. Front. Earth Sci., 2017, 11(4): 682-688.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-016-0629-5
https://academic.hep.com.cn/fesci/EN/Y2017/V11/I4/682

Fig.1 Growth dynamics of P. carterae in the presence of various iron additions in culture with 1 ppm, 5 ppm, and 10 ppm, respectively. Growth curves (a) and specific growth rate (µ) (b). Triplicate cultures at each iron concentration and values are the means of triplicate cultures at each treatment±SD.

Fig.2 Measurements of particulate inorganic carbon contents (PIC) (a), particulate organic carbon contents (POC) (b), and the ratios of PIC/POC (c) ofP. carterae in the presence of various iron additions in culture with 1 ppm, 5 ppm, and 10 ppm, respectively. Triplicate cultures at each iron concentration and values are the means of triplicate cultures at each treatment±SD.

Fig.3 The calcification rate (a), ratios of Sr/Ca (b), and ratios of Mg/Ca (c) of P. carterae in the presence of various iron supplies in culture with 1 ppm, 5 ppm, and 10 ppm, respectively. Triplicate cultures at each Fe concentration and value are the means of triplicate cultures at each treatment±SD.

Fig.4 The UV-Vis spectra of P. carterae in the presence of various iron concentrations in culture with 1 ppm (a), 5 ppm (b), and 10 ppm (c), and compared spectra collected at 120 h culturing (d), respectively.

Fig.5 The ART-FTIR spectra of P. carterae in the presence of various Fe concentrations in culture with normal (a), 1 ppm (b), 5 ppm (c), and 10 ppm (d), respectively.

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