In situ</italic> enhanced bioremediation of dichlorvos by a phyllosphere <italic>Flavobacterium</italic> strain

doi:10.1007/s11783-011-0316-4

Front Envir Sci Eng

2012, Vol. 6

Issue (2) : 231-237 https://doi.org/10.1007/s11783-011-0316-4

RESEARCH ARTICLE

In situ enhanced bioremediation of dichlorvos by a phyllosphere Flavobacterium strain

Jiying NING, Gang GANG, Zhihui BAI, Qing HU, Hongyan QI, Anzhou MA, Xuliang ZHUAN, Guoqiang ZHUANG(

)

Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

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Abstract

A bacterium capable of degrading dichlorvos was isolated from the rape phyllosphere and designated YD4. The strain was identified as Flavobacterium sp., based on its phenotypic features and 16S rRNA gene sequence. Strain YD4 was able to utilize dichlorvos as the sole source of phosphorus. In situ enhanced bioremediation of dichlorvos by YD4 was hereafter studied. Chlorpyrifos and phoxim could also be degraded by this strain as the sole phosphorus source. A higher degradation rate of dichlorvos was observed after spraying YD4 onto the surface of rape leaves when compared to the sterilized-YD4 and water-treated samples. The results indicated that pesticide-degrading epiphytic bacterium could become a new way for in situ phyllosphere bioremediation where the hostile niche is unsuitable for other pesticide-degrading bacteria isolated from soil and water.

Keywords enhanced bioremediation organophosphorus pesticides phyllosphere Flavobacterium sp.

Corresponding Author(s): ZHUANG Guoqiang,Email:gqzhuang@rcees.ac.cn

Issue Date: 01 April 2012

Cite this article:

Gang GANG,Zhihui BAI,Qing HU, et al. In situ enhanced bioremediation of dichlorvos by a phyllosphere Flavobacterium strain[J]. Front Envir Sci Eng, 2012, 6(2): 231-237.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-011-0316-4
https://academic.hep.com.cn/fese/EN/Y2012/V6/I2/231

Fig.1 Unweighted Pair Group Method with Arithmetic(UPGAM) phylogenetic tree based on the 16S rRNA gene sequences of strain YD4. Bootstrap values obtained with 1000 repetitions were indicated as percentages at all branches. GenBank accession numbers are given in brackets

Tab.1 Morphological and biochemical characteristics of dichlorvos-degrading phyllosphere bacterium YD4

Fig.2 Growth kinetics of strain YD4 in MM plus dichlorvos (?), MM containing dichlorvos as a sole source of phosphorus (●), MM containing dichlorvos as a source of carbon (?), and MM containing dichlorvos as sources of carbon and phosphorus (?) at 30°C

Fig.3 Effects of dichlorvos concentration (a), different temperatures (b), and initial pH (c) on dichlorvos degradation by strain YD4

Tab.2 Degradation rate of other organophosphorus pesticides by the isolated YD4

Tab.3 Dichlorvos residue on rape leaves sprayed with water, sterilized-YD4, and YD4

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