Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

doi:10.1007/s11783-011-0320-8

Front Envir Sci Eng Chin

2011, Vol. 5

Issue (2) : 175-185 https://doi.org/10.1007/s11783-011-0320-8

RESEARCH ARTICLE

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE(

), Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

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Abstract

To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of 100? mg-N·kgdrysoil-1 of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, Verrucomicrobia, was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal component analysis (PCA) canonical correspondence analysis (CCA)

Corresponding Author(s): HE Pinjing,Email:solidwaste@tongji.edu.cn

Issue Date: 05 June 2011

Cite this article:

Pinjing HE,Na YANG,Wenjuan FANG, et al. Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium[J]. Front Envir Sci Eng Chin, 2011, 5(2): 175-185.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-011-0320-8
https://academic.hep.com.cn/fese/EN/Y2011/V5/I2/175

Tab.1 Full factorial experiment design

Fig.1 Changes in the CH oxidation rate with time for all soil samples (data are shown as the mean±standard deviation of = 2): (a) ambient oxygen content, and (b) 3% oxygen content

Fig.2 DGGE analysis of Type I targeting 16S rRNA gene fragments generated from ambient O concentration samples: (a) DGGE profile and Shannon index of each lane (only named bands were used to calculate the index) shown below, every lane represents the sample at the conditions illustrated on top of the lane; (b) PCA of DGGE profiles, the numbers before “-” represent the environmental NO., while 30 and 60 after “-” represent a sampling time of 30 days and 60 days, respectively

Fig.3 Neighbor-joining phylogenetic tree of Type I 16S rRNA gene sequences (493 bp) from incubated landfill cover soil. The bar represents 0.02 substitutions per nucleotide position

Fig.4 DGGE analysis of Type II targeting 16S rRNA gene fragments. (a) DGGE profile of the soil samples with ambient O content; (b): DGGE profile of soil samples with a 3% O content, Shannon index of each lane (only named bands were used to calculate the index) were shown below (a) and (b), each lane represents the sample under the conditions illustrated on top of the lane; (c) PCA of DGGE profiles, the numbers before “-” represents the environmental No., while 30 and 60 after “-” represent sampling times of 30 days and 60 days, respectively

Tab.2 Abundance and copy ratios of two types of methanotrophs in different treatments

Fig.5 CCA of the environmental factors and (a) methane oxidation rate patterns along with time; (b) DGGE profiles of Type I methanotrophs; (c) DGGE profiles of Type II methanotrophs. The numbers before “-” represents the environmental No., while 30 and 60 after “-” represent sampling times of 30 days and 60 days, respectively.

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