<i>Pseudomonas mendocina</i> LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously

doi:10.1007/s11783-020-1349-3

Front. Environ. Sci. Eng.

2021, Vol. 15

Issue (4) : 57 https://doi.org/10.1007/s11783-020-1349-3

RESEARCH ARTICLE

Pseudomonas mendocina LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously

Yuxin Li¹, Jiayin Ling¹, Pengcheng Chen¹, Jinliang Chen¹, Ruizhi Dai², Jinsong Liao², Jiejing Yu², Yanbin Xu^1,³(

)

¹. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China
². Guangdong Yikangsheng Environmental Protection Science and Technology Company, Yunfu 527300, China
³. Analysis and Test Center, Guangdong University of Technology, Guangzhou 510006, China

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Abstract

• Pseudomonas mendocina was first reported for aerobic nitrate removal.

• It removed 90% of NO₃⁻-N in 24 h under aerobic conditions.

• This strain converted NO₃⁻-N to bio-nitrogen (37.9%) and gaseous nitrogen (49.7%).

• Inoculation of this strain increased sludge denitrification rate by 4.3 times.

The problem of nitrate accumulation in aerobic tank and total nitrogen excessive discharge in effluent was very common in traditional livestock and poultry farming wastewater treatment systems owing to the lengthy process flow and low process control level. A strain LYX of aerobic bacterium was isolated from the activated sludge of a wastewater treatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas mendocina by 16S rRNA sequencing. Under the condition of nitrate as the sole nitrogen source, this strain removed over 90% of NO₃⁻-N with an initial concentration of 110 mg/L under aerobic conditions within 48 hours. Among them, 37.9% of NO₃⁻-N was assimilated into Bio-N, about 51.9% was reduced to gaseous nitrogen and less than 0.5% of nitrogen was replaced by NO₂⁻-N and NH₄⁺-N, 9.7% NO₃⁻-N remained in the effluent at the end. At the same time, four key genes (napA, nirK, norB and nosZ) related to nitrate nitrogen removal were expressed during the denitrification process of P. mendocina LYX, in which the transcription level of the indicator genes of this aerobic denitrifying bacterium (napA) was the highest. In addition, it was found with the ¹⁵N tracer technique that inoculation of this strain on sludge increased the amount of nitrogen loss from 9.26 nmol N/(g·h) to 23.835 nmol N/(g·h). Therefore, P. medocina LYX is a potential bioagent for advanced nitrogen removal by assimilating and reducing nitrate simultaneously in aerobic tanks.

Keywords Pseudomonas mendocina Aerobic nitrate removal ¹⁵N tracing technique Denitrification assimilatively and disimilatively Aerobic denitrifying genes

Corresponding Author(s): Yanbin Xu

Issue Date: 16 October 2020

Cite this article:

Yuxin Li,Jiayin Ling,Pengcheng Chen, et al. Pseudomonas mendocina LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously[J]. Front. Environ. Sci. Eng., 2021, 15(4): 57.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1349-3
https://academic.hep.com.cn/fese/EN/Y2021/V15/I4/57

Fig.1 Change in the bacterial density and the balance of nitrogen transformation during the cultivation process.

Fig.2 Relative abundance of denitrifying genes during the growth of P. mendocina LYX.

Fig.3 The effect of crude enzyme addition on the removal of nitrate and nitrite nitrogen (the addition quality of crude enzyme was 1g and the reaction time was 15 min).

Fig.4 The rates and amounts of nitrogen removal in different pathways were measured by ¹⁵N tracing technique.

Fig.5 Inference diagram of the denitrification pathway of P. mendocina LYX.

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