Enhancement on the ammonia oxidation capacity of ammonia-oxidizing archaeon originated from wastewater: Utilizing low-density static magnetic field

doi:10.1007/s11783-020-1375-1

Front. Environ. Sci. Eng.

2021, Vol. 15

Issue (5) : 81 https://doi.org/10.1007/s11783-020-1375-1

RESEARCH ARTICLE

Enhancement on the ammonia oxidation capacity of ammonia-oxidizing archaeon originated from wastewater: Utilizing low-density static magnetic field

Zeshen Tian, Bo Wang, Yuyang Li, Bo Shen, Fengjuan Li, Xianghua Wen(

)

State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China

Download: PDF(809 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

• AOA’s ammonia oxidizing capacity was enhanced under moderate magnetic field.

• AOA possessed a certain magnetotaxis under uneven magnetic field.

• Enhanced ammonia oxidizing capacity was lost once magnetic field was removed.

Ammonia-oxidizing archaeon (AOA) could play important roles for nitrogen removal in the bioreactors under conditions such as low pH and low dissolved oxygen. Therefore, enhancing ammonia oxidation capability of AOA has great significance for water and wastewater treatment, especially under conditions like low dissolved oxygen concentration. Utilizing a novel AOA strain SAT1, which was enriched from a wastewater treatment plant by our group, the effect of magnetic field on AOA’s ammonia oxidation capability, its magnetotaxis and heredity were investigated in this study. Compared with control experiment, AOA’s maximum nitrite-N formation rate during the cultivation increased by 56.8% (0.65 mgN/(L·d)) with 20 mT magnetic field. Also, it was testified that AOA possessed a certain magnetotaxis. However, results manifested that the enhancement of AOA’s ammonia oxidation capability was not heritable, that is, lost once the magnetic field was removed. Additionally, the possible mechanism of improving AOA’s ammonia oxidation capability by magnetic field was owing to the promotion of AOA single cells’ growth and fission, rather than the enhancement of their ammonia oxidation rates. The results shed light on the application of AOA and methods to enhance AOA’s ammonia oxidation capability, especially in wastewater treatment processes under certain conditions.

Keywords Ammonia-oxidizing archaeon Ammonia oxidation Magnetic field Magnetotaxis Heredity

Corresponding Author(s): Xianghua Wen

Issue Date: 25 November 2020

Cite this article:

Zeshen Tian,Bo Wang,Yuyang Li, et al. Enhancement on the ammonia oxidation capacity of ammonia-oxidizing archaeon originated from wastewater: Utilizing low-density static magnetic field[J]. Front. Environ. Sci. Eng., 2021, 15(5): 81.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1375-1
https://academic.hep.com.cn/fese/EN/Y2021/V15/I5/81

Fig.1 Schematic diagram of the experimental setup (a: for studying the effect of magnetic field intensity on AOA; b: for studying the magnetotaxis of AOA).

Tab.1 Experimental conditions of batch experiments in current research

Fig.2 Maximum formation rate of nitrite-N as a function of magnetic field intensity. (Note: The ordinate represents the maximum formation rate of nitrite-N among the 17 culture days under a series of magnetic field intensity).

Fig.3 Changes of (a) nitrite-N formation rate and (b) nitrite-N and NH₄-N concentration as a function of time under 0 mT and 20 mT magnetic field.

Fig.4 Number of amoA gene of AOA in an uneven magnetic field at the 11th and 17th culture day.

Fig.5 Concentration of nitrite-N in an uneven magnetic field with intensity gradient at the 11th and 17th culture day.

Fig.6 Changes of formation rate of nitrite-N in three successive batches of culturing AOA under 0 mT and 20 mT magnetic field.

Fig.7 Abundance of archaeal amoA gene in even magnetic fields with different intensities during a 17-day operation period.

Tab.2 Maximum ammonia oxidation rate of single AOA cell as a function of magnetic field intensity

1	J M Beman, B N Popp, S E Alford (2012). Quantification of ammonia oxidation rates and ammonia-oxidizing archaea and bacteria at high resolution in the Gulf of California and eastern tropical North Pacific Ocean. Limnology and Oceanography, 57(3): 711–726 https://doi.org/10.4319/lo.2012.57.3.0711
2	A E Bernhard, A Bollmann (2010). Estuarine nitrifiers: New players, patterns and processes. Estuarine, Coastal and Shelf Science, 88(1): 1–11 https://doi.org/10.1016/j.ecss.2010.01.023
3	M Blohs, C Eichinger, A Mahnert, A Spang, N Dombrowski, M Krupovic, A Klingl (2019). Archaea—An Introduction. In: Schmidt T M, ed. Encyclopedia of Microbiology (Fourth Edition). Oxford: Academic Press, 243–252
4	C H Chen, H Liang, D W Gao (2019). Community diversity and distribution of ammonia-oxidizing archaea in marsh wetlands in the black soil zone in North-east China. Frontiers of Environmental Science & Engineering, 13(4): 58 https://doi.org/10.1007/s11783-019-1146-z
5	K Ding, X H Wen, L Chen, D S Huang, F Fei, Y Y Li (2014). Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China. Frontiers of Environmental Science & Engineering, 8(5): 693–702 https://doi.org/10.1007/s11783-014-0635-3
6	K Ding, X H Wen, Y Y Li, B Shen, B Zhang (2015). Ammonia-oxidizing archaea versus bacteria in two soil aquifer treatment systems. Applied Microbiology and Biotechnology, 99(3): 1337–1347 https://doi.org/10.1007/s00253-014-6188-3
7	C A Francis, K J Roberts, J M Beman, A E Santoro, B B Oakley (2005). Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proceedings of the National Academy of Sciences of the United States of America, 102(41): 14683–14688 https://doi.org/10.1073/pnas.0506625102
8	J Gao, X Luo, G Wu, T Li, Y Peng (2014). Abundance and diversity based on amoA genes of ammonia-oxidizing archaea and bacteria in ten wastewater treatment systems. Applied Microbiology and Biotechnology, 98(7): 3339–3354 https://doi.org/10.1007/s00253-013-5428-2
9	J Z He, J P Shen, L M Zhang, Y G Zhu, Y M Zheng, M G Xu, H Di (2007). Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environmental Microbiology, 9(9): 2364–2374 https://doi.org/10.1111/j.1462-2920.2007.01358.x
10	Z Jia, J Weng, X Lin, R Conrad (2010). Microbial ecology of archaeal ammonia oxidation: A review. Acta microbiologica Sinica, 50(4): 431–437
11	M Könneke, A E Bernhard, J R de la Torre, C B Walker, J B Waterbury, D A Stahl (2005). Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature, 437(7058): 543–546 https://doi.org/10.1038/nature03911
12	L E Lehtovirta-Morley, C Ge, J Ross, H Yao, G W Nicol, J I Prosser (2014). Characterisation of terrestrial acidophilic archaeal ammonia oxidisers and their inhibition and stimulation by organic compounds. FEMS Microbiology Ecology, 89(3): 542–552 https://doi.org/10.1111/1574-6941.12353
13	Y Li, K Ding, X Wen, B Zhang, B Shen, Y Yang (2016). A novel ammonia-oxidizing archaeon from wastewater treatment plant: Its enrichment, physiological and genomic characteristics. Scientific Reports, 6(1): 23747 https://doi.org/10.1038/srep23747
14	J J Liu, W X Wu, Y Ding, D Z Shi, Y X Chen (2010). Ammonia-oxidizing archaea and their important roles in nitrogen biogeochemical cycling: A review. Chinese Journal of Applied Ecology, 21(8): 2154–2160 (in Chinese) https://doi.org/10.13287/j.1001-9332.2010.0276
15	S Liu, F Yang, F Meng, H Chen, Z Gong (2008). Enhanced anammox consortium activity for nitrogen removal: Impacts of static magnetic field. Journal of Biotechnology, 138(3–4): 96–102 https://doi.org/10.1016/j.jbiotec.2008.08.002
16	F Ma, Q Wang, X Zhu, J Shan, C Du, J Sun (2010). Application status and development trend of magnetic technology in wastewater treatment. China Water & Wastewater, 26(14): 34–37 (in Chinese) https://doi.org/10.19853/j.zgjsps.1000-4602.2010.14.008
17	C Niu, W Liang, H Ren, J Geng, L Ding, K Xu (2014). Enhancement of activated sludge activity by 10–50 mT static magnetic field intensity at low temperature. Bioresource Technology, 159: 48–54 https://doi.org/10.1016/j.biortech.2014.01.131
18	H D Park, G F Wells, H Bae, C S Criddle, C A Francis (2006). Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors. Applied Microbiology and Biotechnology, 72(8): 5643–5647 https://doi.org/10.1128/aem.00402-06
19	A Pitcher, N Rychlik, E C Hopmans, E Spieck, W I C Rijpstra, J Ossebaar, S Schouten, M Wagner, J S Sinninghe Damsté (2010). Crenarchaeol dominates the membrane lipids of Candidatus Nitrososphaera gargensis, a thermophilic Group I.1b Archaeon. ISME Journal, 4(4): 542–552 https://doi.org/10.1038/ismej.2009.138
20	W Qin, S A Amin, W Martens-Habbena, C B Walker, H Urakawa, A H Devol, A E Ingalls, J W Moffett, E V Armbrust, D A Stahl (2014). Marine ammonia-oxidizing archaeal isolates display obligate mixotrophy and wide ecotypic variation. Proceedings of the National Academy of Sciences of the United States of America, 111(34): 12504–12509 https://doi.org/10.1073/pnas.1324115111
21	T Rao, R L Sonolikar, S P Saheb (1997). Influence of magnetic field on the performance of bubble columns and airlift bioreactor with submersed microorganisms. Chemical Engineering Science, 52(21–22): 4155–4160 https://doi.org/10.1016/S0009-2509(97)00235-2
22	A D Rosen (2003). Mechanism of action of moderate-intensity static magnetic fields on biological systems. Cell Biochemistry and Biophysics, 39(2): 163–174 https://doi.org/10.1385/CBB:39:2:163
23	A E Santoro, C L Dupont, R A Richter, M T Craig, P Carini, M R McIlvin, Y Yang, W D Orsi, D M Moran, M A Saito (2015). Genomic and proteomic characterization of “Candidatus Nitrosopelagicus brevis”: An ammonia-oxidizing archaeon from the open ocean. Proceedings of the National Academy of Sciences of the United States of America, 112(4): 1173–1178 https://doi.org/10.1073/pnas.1416223112
24	A Sims, J Horton, S Gajaraj, S McIntosh, R J Miles, R Mueller, R Reed, Z Hu (2012). Temporal and spatial distributions of ammonia-oxidizing archaea and bacteria and their ratio as an indicator of oligotrophic conditions in natural wetlands. Water Research, 46(13): 4121–4129 https://doi.org/10.1016/j.watres.2012.05.007
25	S Spring, K H Schleifer (1995). Diversity of magnetotactic bacteria. Systematic and Applied Microbiology, 18(2): 147–153 https://doi.org/10.1016/S0723-2020(11)80386-3
26	M Tourna, T E Freitag, G W Nicol, J I Prosser (2008). Growth, activity and temperature responses of ammonia-oxidizing archaea and bacteria in soil microcosms. Environmental Microbiology, 10(5): 1357–1364 https://doi.org/10.1111/j.1462-2920.2007.01563.x
27	M Tourna, M Stieglmeier, A Spang, M Konneke, A Schintlmeister, T Urich, M Engel, M Schloter, M Wagner, A Richter, C Schleper (2011). Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil. Proceedings of the National Academy of Sciences of the United States of America, 108(20): 8420–8425 https://doi.org/10.1073/pnas.1013488108
28	Y Yavuz, S S Celebi (2003). A typical application of magnetic field in wastewater treatment with fluidized bed biofilm reactor. Chemical Engineering Science, 190(5–8): 599–609 https://doi.org/10.1080/00986440390207288
29	N S Zaidi, J Sohaili, K Muda, M Sillanpaa (2014). Magnetic field application and its potential in water and wastewater treatment systems. Separation and Purification Technology, 43(3): 206–240 https://doi.org/10.1080/15422119.2013.794148

[1]	Yukun Zhang, Shuying Wang, Shengbo Gu, Liang Zhang, Yijun Dong, Lei Jiang, Wei Fan, Yongzhen Peng. The combined effects of biomass and temperature on maximum specific ammonia oxidation rate in domestic wastewater treatment[J]. Front. Environ. Sci. Eng., 2021, 15(6): 123-.
[2]	Hong YAO, Hao LIU, Yongmiao HE, Shujun ZHANG, Peizhe SUN, Chinghua HUANG. Performance of an ANAMMOX reactor treating wastewater generated by antibiotic and starch production processes[J]. Front Envir Sci Eng, 2012, 6(6): 875-883.

Viewed

Full text

Abstract

Cited

Shared

Discussed