Innovative method of culturing bdelloid rotifers for the application of wastewater biological treatment

doi:10.1007/s11783-021-1477-4

Front. Environ. Sci. Eng.

2022, Vol. 16

Issue (4) : 43 https://doi.org/10.1007/s11783-021-1477-4

RESEARCH ARTICLE

Innovative method of culturing bdelloid rotifers for the application of wastewater biological treatment

Yun He¹, Jianyong Liu¹, Chengyuan Shen^1,², Xuewen Yi^1,³, Xiaowei Li¹, Xin Huang¹, Kokyo Oh⁴, Guoji Ding¹(

)

¹. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
². Shanghai Environmental School, Shanghai 200135, China
³. Shanghai Jinshan Municipal Bureau of Ecology and Environment, Shanghai 200540, China
⁴. Center for Environmental Science in Saitama, Saitama 347-0115, Japan

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Abstract

• An innovative method of culturing bdelloid rotifer fed on flour was proposed.

• Rotifer fed on flour grew faster than that fed on bacteria or Chlorella vulgaris.

• The optimum mass culture conditions for rotifer fed on flour were investigated.

• The cultured rotifer could improve sludge settleability in the SBR.

This study aims to establish a simple and efficient method for the mass culture of bdelloid rotifers, which is the basis for the application of bdelloid rotifers as biological manipulators to improve wastewater biological treatment performance. A common bdelloid rotifer, Habrotrocha sp., in a wastewater biological treatment system was selected as the culture target. Rotifers fed on flour could reproduce faster than those fed traditional food such as Chlorella vulgaris or mixed bacteria. As a rotifer food, flour has the advantages of simple preparation, effortless preservation, and low cost compared to live Chlorella vulgaris or mixed bacteria, so it is more suitable for the mass culture of rotifers. The optimal rotifer culture conditions using flour as food were also studied. According to the experimental results, the recommended rotifer culture conditions are a flour particle size of 1 μm, a flour concentration of 6 × 10⁶ cell/mL, a temperature of 28℃, a pH level of 6.5 and salinity of 100–500 mg/L. In addition, the sludge volume index in the sequencing batch reactor (SBR) with the addition of cultured rotifers was 59.9 mL/g at the end of operation and decreased by 18.2% compared to SBR without rotifer, which indicates that the cultured rotifers still retained the function of helping to improve sludge settling. This function may be related to the rotifer’s role in inhibiting bacteria from producing loosely bound extracellular polymeric substances in the SBR.

Keywords Mass culture Bdelloid rotifer Habrotrocha Wheat flour Sludge settleability

Corresponding Author(s): Guoji Ding

Issue Date: 27 July 2021

Cite this article:

Yun He,Jianyong Liu,Chengyuan Shen, et al. Innovative method of culturing bdelloid rotifers for the application of wastewater biological treatment[J]. Front. Environ. Sci. Eng., 2022, 16(4): 43.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-021-1477-4
https://academic.hep.com.cn/fese/EN/Y2022/V16/I4/43

Tab.1 Characteristics of particle size distribution and natural sedimentation of five grades of particles

Fig.1 Population growth characteristics of Habrotrocha sp. cultured with three different foods.

Fig.2 Population growth characteristics of Habrotrocha sp. cultured with five different flour particle sizes. Abbreviations: S_1–5, S1, S2, S3, S4, and S5 group. Points are the mean±standard error based on three replicates. The inset shows the relationship between particle size and SGR of Habrotrocha sp.

Fig.3 Population growth characteristics of Habrotrocha sp. fed on five different food concentrations. Points are the mean±standard error based on three replicates. Abbreviations: C_1–5, C1, C2, C3, C4, and C5 group. The inset shows the relationship between flour concentration and SGR of Habrotrocha sp.

Fig.4 Population growth characteristics of Habrotrocha sp. under different growth environmental conditions: (a) temperature; (b) pH and (c) salinity. Points are the mean±standard error based on three replicates

Fig.5 Settleability of the sludge (a); LB-EPS and TB-EPS of sludge in SBR (b) with and without Habrotrocha sp. Points are the mean±standard error based on three replicates.

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