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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

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Front. Environ. Sci. Eng.    2022, Vol. 16 Issue (6) : 69    https://doi.org/10.1007/s11783-021-1503-6
RESEARCH ARTICLE
A heterotrophic nitrification-aerobic denitrification bacterium Halomonas venusta TJPU05 suitable for nitrogen removal from high-salinity wastewater
Quanli Man1, Peilian Zhang1, Weiqi Huang1, Qing Zhu1, Xiaoling He1,2(), Dongsheng Wei3()
1. State Key Laboratory of Separation Membrane and Membrane Process, School of Environmental Science and Engineering, Tiangong University, Tianjin 300387, China
2. School of Chemistry, Tiangong University, Tianjin 300387, China
3. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China
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Abstract

• H. venusta TJPU05 showed excellent HN-AD ability at high salinity.

• Successful expression of AMO, HAO, NAR and NIR confirmed the HN-AD ability of TJPU05.

• H. venusta TJPU05 could tolerate high salt and high nitrogen environment.

• H. venusta TJPU05 is a promising candidate for the bio-treatment of AW.

A novel salt-tolerant heterotrophic nitrification and aerobic denitrification (HN-AD) bacterium was isolated and identified as Halomonas venusta TJPU05 (H. venusta TJPU05). The nitrogen removal performance of H. venusta TJPU05 in simulated water (SW) with sole or mixed nitrogen sources and in actual wastewater (AW) with high concentration of salt and nitrogen was investigated. The results showed that 86.12% of NH4+-N, 95.68% of NO3–-N, 100% of NO2–-N and 84.57% of total nitrogen (TN) could be removed from SW with sole nitrogen sources within 24 h at the utmost. H. venusta TJPU05 could maximally remove 84.06% of NH4+-N, 92.33% of NO3–-N, 92.9% of NO2–-N and 77.73% of TN from SW with mixed nitrogen source when the salinity was above 8%. The application of H. venusta TJPU05 in treating AW with high salt and high ammonia nitrogen led to removal efficiencies of 50.96%, 47.28% and 43.19% for NH4+-N, NO3–-N and TN respectively without any optimization. Furthermore, the activities of nitrogen removal–related enzymes of the strain were also investigated. The successful detection of high level activities of ammonia oxygenase (AMO), hydroxylamine oxidase (HAO), nitrate reductase (NAR) and nitrite reductase (NIR) enzymes under high salinity condition further proved the HN-AD and salt-tolerance capacity of H. venusta TJPU05. These results demonstrated that the H. venusta TJPU05 has great potential in treating high-salinity nitrogenous wastewater.
Keywords Salt-tolerant bacteria      H. venusta TJPU05      Heterotrophic nitrification and aerobic denitrification      High-salinity wastewater     
Corresponding Author(s): Xiaoling He,Dongsheng Wei   
Issue Date: 23 September 2021
 Cite this article:   
Quanli Man,Peilian Zhang,Weiqi Huang, et al. A heterotrophic nitrification-aerobic denitrification bacterium Halomonas venusta TJPU05 suitable for nitrogen removal from high-salinity wastewater[J]. Front. Environ. Sci. Eng., 2022, 16(6): 69.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-021-1503-6
https://academic.hep.com.cn/fese/EN/Y2022/V16/I6/69
Characteristics Results
pH 8.30
COD (mg/L) 3878±0.3
TN (mg/L) 1615±57
TDS (mg/L) 1.93 × 104
NH4+-N (mg/L) 1572±5
NO3–-N (mg/L) 45±1
NO2–-N (mg/L) nd
Tab.1  Characteristics of AW used in this study
Fig.1  Colony morphology on LB medium plate (A) and corresponding single cell morphology observed by SEM (B) of H. venusta TJPU05. Phylogenetic tree based on the neighboring joining method of 16S rDNA gene sequences (C).
Fig.2  Nitrogen removal characteristics of H. venusta TJPU05 in HNM medium under different salinity after incubation for 24 h. Statistical significance of data at 5%, 8% and 10% salinity compared with those at 2.5% salinity was evaluated, respectively; * P<0.05.
Fig.3  Effects of salt concentration in the medium on the N removal-related enzyme activity of H. venusta TJPU05. Statistical significance of data at 5%, 8% and 10% salinity compared with those at 2.5% salinity was evaluated, respectively; * P<0.05.
Fig.4  Nitrogen removal performance of H. venusta TJPU05 in ADM-1 (A) and ADM-2 (B) medium under different salinity after incubation for 24 h. Statistical significance of data at 5%, 8% and 10% salinity compared with those at 2.5% salinity was evaluated, respectively; * P<0.05.
Fig.5  Nitrogen removal performance of H. venusta TJPU05 in SND-1 (A) and SND-2 (B) medium under different salinity after incubation for 24 h. Statistical significance of data at 5%, 8% and 10% salinity compared with those at 2.5% salinity was evaluated, respectively; * P<0.05.
Fig.6  The removal performance of NH4+-N, NO3–-N and TN by H. venusta TJPU05 in AW. Statistical significance of data between control group and experimental group was evaluated: * P<0.05.
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