An antibiotic composite electrode for improving the sensitivity of electrochemically active biofilm biosensor

doi:10.1007/s11783-022-1518-7

Front. Environ. Sci. Eng.

2022, Vol. 16

Issue (8) : 97 https://doi.org/10.1007/s11783-022-1518-7

RESEARCH ARTICLE

An antibiotic composite electrode for improving the sensitivity of electrochemically active biofilm biosensor

Shuyi Wang¹, Xiang Qi¹, Yong Jiang²(

), Panpan Liu³, Wen Hao¹, Jinbin Han¹, Peng Liang¹(

)

¹. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China
². Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
³. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China

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Abstract

• Antibiotic azithromycin employed in graphite electrode for EAB biosensor.

• Azithromycin at 0.5% dosage increased the sensitivity for toxic formaldehyde.

• Azithromycin increased the relative abundance of Geobacter.

• Azithromycin regulated thickness of electroactive biofilm.

Extensive research has been carried out for improved sensitivity of electroactive biofilm-based sensor (EAB-sensor), which is recognized as a useful tool in water quality early-warning. Antibiotic that is employed widely to treat infection has been proved feasible in this study to regulate the EAB and to increase the EAB-biosensor’s sensitivity. A novel composite electrode was prepared using azithromycin (AZM) and graphite powder (GP), namely AZM@GP electrode, and was employed as the anode in EAB-biosensor. Different dosages of AZM, i.e., 2 mg, 4 mg, and 8 mg, referred to as 0.25%, 0.5% and 1% AZM@GP were under examination. Results showed that EAB-biosensor was greatly benefited from appropriate dosage of AZM (0.5% AZM@GP) with reduced start-up time period, comparatively higher voltage output, more readable electrical signal and increased inhibition rate (30%-65% higher than control sensor with GP electrode) when exposing to toxic formaldehyde. This may be attributed to the fact that AZM inhibited the growth of non-EAM without much influence on the physiologic or metabolism activities of EAM under proper dosage. Further investigation of the biofilm morphology and microbial community analysis suggested that the biofilm formation was optimized with reduced thickness and enriched Geobacter with 0.5% AZM@GP dosage. This novel electrode is easily fabricated and equipped, and therefore would be a promising way to facilitate the practical application of EAB-sensors.

Keywords AZM@GP composite electrode EAB-biosensor Water quality early-warning

Corresponding Author(s): Yong Jiang,Peng Liang

About author:

Tongcan Cui and Yizhe Hou contributed equally to this work.

Issue Date: 08 December 2021

Cite this article:

Shuyi Wang,Xiang Qi,Yong Jiang, et al. An antibiotic composite electrode for improving the sensitivity of electrochemically active biofilm biosensor[J]. Front. Environ. Sci. Eng., 2022, 16(8): 97.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-022-1518-7
https://academic.hep.com.cn/fese/EN/Y2022/V16/I8/97

Fig.1 (A) Preparation principle of AZM@GP, (B) true-color photographs and SEM images of GP and 1% AZM@GP electrode, (C) CV curves of GP and AZM@GP composite electrode, (D) EIS plots of GP and AZM@GP composite electrode.

Fig.2 (A) Schematic diagram of the built EAB-sensor, (B) Electrical signal output during start-up.

Fig.3 Toxicity tests of formaldehyde under different concentrations. NES changes within 30 min under 0.01% (A), 0.02% (B), 0.03% (C) formaldehyde, respectively and (D) corresponding inhibition rates at 30 min.

Fig.4 (A) Front view of CLSM images, (B) calculated biofilm thickness, (C) SEM images of EABs formed on different electrodes.

Fig.5 Microbial community analyses of EABs. (A) Chao 1 indicator, (B) relative abundance of microbial community.

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