Carbon isotope discrimination in leaf juice of <italic>Acacia mangium</italic> and its relationship to water-use efficiency

doi:10.1007/s11461-009-0027-1

Front Fore Chin

2009, Vol. 4

Issue (2) : 201-207 https://doi.org/10.1007/s11461-009-0027-1

RESEARCH ARTICLE

Carbon isotope discrimination in leaf juice of Acacia mangium and its relationship to water-use efficiency

Lvliu ZOU, Guchou SUN, Ping ZHAO(

), Xian CAI, Xiaoping ZENG, Xiaojing LIU

South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China

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Abstract

Using the PMS pressure chamber and isotope mass spectrometer (MAT-252), the leaf juice of Acacia mangium was obtained, and the carbon isotope discrimination (D) representing the most recently fixed carbon in the juice was determined. At the same time, the water-use efficiency of A. mangium was estimated. The results indicated that the carbon isotope ratio in the air of forest canopy (d_a), 10 m high above ground averaged -7.57±1.41‰ in cloudy days, and -8.54±0.67‰ in sunny days, respectively. The diurnal change of the carbon isotope ratio in the photosynthetic products of the leaf juice (d_p) was of saddle type in cloudy days, but dropped down from morning to later afternoon in sunny days. A strong negative correlation between d_p and leaf-to-air vapor pressure deficit (D) was observed in sunny days, but a slight change in d_p was found in cloudy days. The d_p also decreased with decreasing leaf water potential (Ψ), reflecting that water stress could cause the decrease of d_p. The carbon isotope discrimination of the leaf juice was positively correlated with the ratio between intercellular (P_i) and atmospheric (P_a) partial pressure of CO₂. For A. mangium, the isotope effect on diffusion of atmospheric CO₂ via stomata was denoted by a = 4.6‰, and that in net C₃ diffusion with respect to P_i was indicated by b = 28.2‰. The results were in reasonable accord with the theoretically diffusive and biochemical fractionation of carbon isotope. It was defined that carbon isotope discrimination of photosynthetic products in A. mangium leaf juice was in proportion to that from photosynthetic products in dry material. The water-use efficiency estimated by the carbon isotope discrimination in leaf juice, fit well with that measured by gas exchange system (R²= 0.86, p< 0.0001). The application of leaf juice in measuring the stable carbon isotope discrimination would reduce the effects of fluctuating environmental factors during the synthesis of dry matter, and improve the eco-physiological studies on carbon and water balance when scaling from the plant to canopy in the fields.

Keywords carbon isotope discrimination water-use efficiency photosynthetic products in leaf juice

Corresponding Author(s): ZHAO Ping,Email:zhaoping@scib.ac.cn

Issue Date: 05 June 2009

Cite this article:

Lvliu ZOU,Guchou SUN,Ping ZHAO, et al. Carbon isotope discrimination in leaf juice of Acacia mangium and its relationship to water-use efficiency[J]. Front Fore Chin, 2009, 4(2): 201-207.

URL:

https://academic.hep.com.cn/ffc/EN/10.1007/s11461-009-0027-1
https://academic.hep.com.cn/ffc/EN/Y2009/V4/I2/201

Fig.1 Relationship between carbon isotope ratio () and CO concentration of air in the canopy of plantation
Note: (a), (c): cloudy day; (b), (d): sunny day.

Fig.2 Daily changes of light intensity, leaf temperature and leaf-to-air vapor pressure difference (a, b), carbon isotope ratio (c, d) of photosynthetic product in leaves of
Note: cloudy day: a, c; sunny day: b, d.

Fig.3 The relationship between leaf-to-air vapor pressure deficit () and carbon isotope ratio () of photosynthetic products in leaves of
Note: Cloudy day with average irradiance of 290 μmol/(m·s) and mean leaf temperature of 19.9±2.1°C; Sunny day with average irradiance of 536 μmol/(m·s) and mean leaf temperature of 23.5±3.6°C.

Fig.4 The relationship between leaf water potential () and carbon isotope ratio () of photosynthetic products in leaves of
Note: Ambient CO partial pressure was 38.6 Pa; leaf-to-air vapor pressure deficit was 1.46 kPa.

Fig.5 Carbon isotope discrimination in relationship to the ratio between intercellular and ambient CO partial pressure (/)
Note: and were measured with gas exchange system, was calculated from Eq. (3).

Fig.6 Relationship between water-use efficiency (WUE) and leaf carbon isotope discrimination () of .
Note: WUE data were obtained from Fig. 2, ambient CO partial pressure was 38.6 Pa, leaf-to-air vapor pressure deficit was 1.46 kPa.

Fig.7 Leaf water-use efficiency (WUE) value calculated from the measurement using CO exchange method in comparison with that based on carbon stable isotope measurement of photosynthetic product of A. mangium (WUE)
Note: WUE values on -axis were calculated from using equation 6, values of and are 4.4‰ and 27‰ respectively in Fig. 7(a), while they are 4.6‰ and 28.2‰ respectively obtained from this issue Fig. 7(b).

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