Plants initiate the development of defense mechanisms as soon as pests start to cause damage to them. In order to have a thorough understanding of the physiological mechanisms of the Pinus massoniana self-defense mechanism, and to provide a theoretical foundation for an effective ecological management of this plant, levels of tannin, polyamine and phenolic acids were analyzed in undamaged (UDL), insect-damaged (IDL) and artificially-damaged (ADL) leaves at different times. Results show that, although the content of tannin significantly increased in IDL and ADL compared to UDL, its peaks appeared earlier in ADL than in IDL treatment. Tannin concentration substantially increased again 48 h after IDL treatment. On the other hand, the damage mode considerably affected putrescine and spermidine levels in leaves. Their concentrations in IDL plants remained higher than in UDL after a relatively long time (72 h), but spermine was barely detected in any of the samples. In general, total content of phenol acids significantly increased in damaged leaf treatments (ADL and IDL), with a higher level in IDL for most of the investigated phenolic acids, except for ferulic acid. Our study showed that, when damaged by insects, Pinus massoniana rapidly produces substances required in resistance induction to insects in order to insure its self-protection.

In the intermediate cutting intensity experiment of a Cunninghamia lanceolata plantation for 20 years, the changing pattern of natural thinning in these stands, with different intermediate cutting intensities, was studied. The relationship between the number of trees removed by natural thinning and stand density and site conditions was explained. The mathematical equation M = K₁K₂ of natural thinning lines of C. lanceolata stand density management maps was tested and the relationship of diameter, height and canopy structure of stands with different intermediate cutting intensities are proposed. Our study of natural thinning in these stands indicates that the starting and peak periods of natural thinning in the check and slightly thinned plots were both early. The amount of thinned wood was large and the course of thinning proceeded continuously. The three levels of thinning: the slight thinning period, the intensive thinning period and the continued thinning period could be divided on the basis of the amount of thinned wood. Natural thinning would be a very long process without artificial interference. The starting and peak periods of thinning in the middle and strong intermediate felling are both late and present intermittence. Their thinning stages were not clearly evident. Through our studies, we also discovered that stand density and site conditions had important effects on the number of dead and dying trees, but that density was more important than site conditions. By way of tests, the relative error of the mathematical equation of natural thinning lines of C. lanceolata stand density management maps was 3.91% and the precision was relatively high. The practical test results of the stands, given different intermediate cutting intensities and different site indices, show that the relative error of the check plots was 5.23%, while the relative errors of the other tested items were all < 5%, well within the allowable experimental error. The mathematical equation was comparatively practical. The study demonstrated the distribution laws of diameter and height classes of the stand at different intermediate cutting intensities. From this study we also obtained the growth differences and changing dynamics of the height to the first branch, canopy length and relative canopy height of the stand at different intermediate cutting intensities and various related patterns with an increase of stand age and proposed a mathematical model relating stand age and the single-tree periodic volume increment.

The functions of canopy interception on energy conversion processes in a Chinese fir plantation ecosystem were studied with the aid of long-term observation data in Huitong. The results showed that the absorbed, penetrated and reflected amounts of solar radiation were, respectively, 2.5543 × 10⁹ J/(m²year) (absorption rate of 0.827), 2.5306 × 10⁸ J/(m²year) (penetration rate of 0.082), and 2.7432 × 10⁸ J/(m²year) (reflection rate of 0.091) by the canopy. The conversion of net solar radiation to latent heat in the process of evaporation from canopy interception amounted to 6.3695 × 10⁸ J/(m²year) (accounting for 22.9% of total ecosystem net radiation and 30.4% of ecosystem evaporation), which was an important part of the budget of the energy system. Canopy interception consumed kinetic energy of raindrops in overcoming resistance of branches and leaves, which collected raindrops, followed with the conversion of potential energy in raindrops to kinetic energy with falling raindrops. In general, the diameter of raindrops from the canopy is larger than that of the raindrops above the canopy as a result of the collection effort by the canopy. The kinetic energy of raindrops from the canopy, therefore, was higher than that of raindrops in the atmosphere. The drop-size distribution from the canopy was affected by the structure of the canopy layer rather than the amount of precipitation and precipitation intensity. The canopy had no important nor efficient effects on decreasing the kinetic energy of raindrops in our case study with a first branch height of 7 m and precipitation amounts over 3 mm. However, the canopy would play a key role in decreasing kinetic energy of raindrops in two cases, that of a small amount of precipitation and one of heavy precipitation intensity, in which the canopy could intercept the largest amount of precipitation in the former condition and the canopy could scatter bigger raindrops to smaller raindrops with striking leaves in the case of heavy precipitation.

Using a strip transect sampling method, the density, height (≤100 cm), basal diameter and components of biomass of Abies faxoniana seedlings, living in a forest gap (FG) and under the forest canopy (FC) of subalpine natural coniferous forests in western Sichuan, were investigated and the relationships among different components of biomass analyzed. The results indicated that the density and average height (H) of A. faxoniana seedlings were significantly different in the FG and under the FC, with the values being 12903 and 2017 per hm², and 26.6 and 24.3 cm. No significant differences were found in the average basal diameter (D) and biomass. The biomass allocation in seedling components was significantly affected by forest gap. In the FG, the biomass ratio of branch to stem reached a maximum of 1.54 at age 12 and then declined and fluctuated around 0.69. Under the FC, the biomass ratio of branch to stem increased with seedling growth and exceeded 1.0 at about age 15. The total biomass and the biomass of leaves, stems, shoots and roots grown in the FG and under the FC were significantly correlated with D²H. There were significant and positive correlations among the biomass of different components.

Reforestation is one of the most important and efficient measures of water and soil conservation. Based on field investigations in the Shangyang Soil Conservation and Reforestation Station in Huizhou, Guangdong Province, China, we studied the variation in vegetation development, vegetation succession processes and soil erosion. The regional vegetation consists mainly of monsoon evergreen broad-leaved forests (MEBF). The area was deforested and became a denuded hill area with extremely high soil erosion in the 1960s and 1970s. Then, the area was closed in order to allow recovery of the vegetation. Under natural conditions the vegetation development and succession processes were slow during which soil erosion and strong sunshine and evaporation slowed down the development of the vegetation. About 25 years later, the vegetation cover was still merely 35% or so. The dominant vegetation types were heliophilous herbage and shrubs which formed a poorly developed shrub-herbage community and erosion remained high. In contrast, reforestation with selected tree species dramatically speeded up the vegetation succession process. About 12 years after reforestation, vegetation cover of the Acacia auriculiformis plantations in the Shangyang Station was 90% and erosion was under control. After 23 years, understory vegetation, consisting of indigenous species, had developed in the plantations. The planted trees and naturally developing herbage, shrubs, bamboo, local trees and liana formed a complex vegetation community in three layers. It will take 60 years for the vegetation to succeed from bare land to a secondary growth forest under natural conditions. Reforestation may speed up the vegetation succession process. The time may be reduced to 20 years. Reforestation is the most effective measure of vegetation restoration and erosion control in this area.

Aerial seeding is one of the most important vegetation restoration patterns in remote hilly areas, and studies concerning soil quality and its management have practical value. In 2000, a study of the effect of thinning intensities at five different treatments levels, 0 (CK), 30% (slight thinning), 48.75% (medium thinning), 53.75% (intense thinning) and 65.6% (super intense thinning) on soil enzyme activity was carried out on 9-year-old aerial seeded Chinese pine (Pinus tabulaeformis Carr.) stands with an initial density of 8000 trees/hm², in the Wangjiapu Aerail Seeding Center, Yanqing County, Beijing. Five years later, the activities of five kinds of soil enzymes, soil urease, alkaline phosphatase, inertase, catalase and polyphenol oxidase in the first 20 cm of soil layer were compared during four seasons. Relationships among soil enzymes and soil physiochemical properties were also analyzed to examine the possibility of using soil enzymes to evaluate thinning intensities. The results showed that the maximum enzyme activities of catalase and polyphenol oxidase occurred in June, those of soil urease and alkaline phosphatase occurred in October, and soil invetase had its maximum in April. In addition, the five soil enzymes were affected differently by thinning intensities. Soil catalase, urease and invertase showed the highest response to a slight thinning, followed by medium thinning, which is the opposite experienced with polyphenol oxidase and alkaline phosphatase. There are statistically significant and positive relationships between soil enzymes and organic matter and available K. It should be noted that soil water was a limiting factor to soil enzyme activity. Compared with soil physicochemical characteristics, soil enzymes were more sensitive to levels of thinning intensities. Among the enzymes, soil alkaline phosphatase and catalase could be regarded as indicators to assess soil quality. It is concluded that a suitable thinning intensity benefits the development of undergrowth and soil enzymes. Generally, when the stand with initial density of 8000 trees/hm² grows up to nine years old, the most suitable thinning intensity should be about 50%.

Vegetation recovery is a key measure to improve ecosystems in the Loess Plateau in China. To understand the evolution of soil microorganisms in forest plantations in the hilly areas of the Loess Plateau, the soil microbial biomass, microbial respiration and physical and chemical properties of the soil of Robinia pseudoacacia plantations were studied. In this study, eight forest soils of different age classes were used to study the evolution of soil microbial biomass, while a farmland and a native forest community of Platycladus orientalis L. were chosen as controls. By measuring soil microbial biomass, metabolic quotient, and physical and chemical properties, it can be concluded that soil quality was improved steadily after planting. Soil microbial biomass of C, N and P (SMBC, SMBN and SMBP) increased significantly after 10 to 15 years of afforestation and vegetation recovery. A relatively stable state of soil microbial biomass was maintained in near-mature or mature plantations. There was an increase of soil microbial biomass appearing at the end of the mature stage. After 50 years of afforestation and vegetation recovery, compared with those in farmland, the soil microbial biomass of C, N and P increased by 213%, 201% and 83% respectively, but only accounting for 51%, 55% and 61% of the increase in P. orientalis forest. Microbial soil respiration was enhanced in the early stages, and then weakened in the later stage after restoration, which was different from the change of soil organic carbon. The metabolic quotient (qCO₂) was significantly higher in the soils of the P. orientalis forest than that in farmland at the early restoration stage and then decreased rapidly. After 25 years of afforestation and vegetation recovery, qCO₂ in soils of the R. pseudoacacia forest was lower than that in the farmland soil, and reached a minimum after 50 years, which was close to that of the P. orientalis forest. A significant relationship was found among soil microbial biomass, qCO₂ and physical and chemical properties and restoration duration. Therefore, we conclude that it is possible to artificially improve the ecological environment and soil quality in the hilly area of the Loess Plateau; a long time, even more than 100 years, is needed to reach the climax of the present natural forest.

By adopting the concept of space as a substitute for time, we analyzed the dynamics of species composition and diversity of different restoration sequences (20, 30, 40, 50 years) in two secondary forest types in western Sichuan Province, distributed in a northerly or northwesterly direction. The analysis was based on the results of measurements of 50 plots located at elevations between 3100–3600 m. The forests originated from natural regeneration in combination with reforestation of spruce when the old-growth bamboo-dark brown coniferous forests and moss-dark brown coniferous old growth forests were harvested. Similar old-growth dark brown coniferous forests at ages ranging between 160 and 200 years were selected as the reference forests for comparisons. We recorded 167 species of vascular plants from 44 families and 117 genera. There was no significant difference in terms of the number of species among secondary forests. But the importance values of dominant species varied during the restoration processes. The dominant species in the secondary forests is Betula albo-sinensis, while Abies faxoniana is the dominant species in old-growth dark brown coniferous forests. Species richness increased significantly with restoration processes. It increased quickly in secondary forests during the period from 30 to 40 years, but decreased significantly in the old-growth dark brown coniferous forests. The species richness among growth forms decreased in the following order: herb layer > shrub layer > tree layer. The maximum value of the evenness index occurred in secondary forests at age 40 and remained relatively stable in the bamboo-birch forests, but the evenness index tended to decrease in moss-birch forests and slightly increased in the old-growth moss-dark brown coniferous forests. There was a statistically significant difference in the evenness index between the tree and shrub layers as well as between the tree layer and the herb layer, but there was no significant difference between the shrub layer and the herb layer. The value of the Shannon index increased over restoration time. In bamboo-birch forests, the maximum value of the Shannon index was 3.80, recorded at age 50. In moss-birch forests, the maximal value was 3.65, reached in this forest at age 30. The value of the Shannon index of old-growth dark brown coniferous forests was recorded between younger secondary and older secondary forests. The value of the dominance index of communities varied. At the first stage of restoration, it increased, and at the end it was decreased. The dominance index of the tree layer had a similar trend as that of the community dominance index, but was more variable. The minimum value of the dominance index of the tree layer in the moss-birch forests reached 20 years earlier than that of the bamboo-birch forests. There was a significant difference among restoration sequences in the ? diversity indices except for the dominance index. No significant differences between the two secondary forest types were detected. Over age, the value of the Bray-Curtis index between secondary forest and old-growth dark brown coniferous forest increased.

Species association is one of the basic concepts in community succession. There are different viewpoints on how species interaction changes with the progress of succession. In order to assess these relationships, we examined species associations in the tropical montane rain forest at early and late successional stages in Diaoluo Mountain, Hainan Island. Based on data from a 2 × 2 contingency table of species presence or absence, statistical methods including analysis of species association and ?² tests were applied. The results show that: 1) an overall positive association was present among tree species in the communities during the two successional stages and were statistically significant at the late stage. The number of species pairs with positive and negative associations decreased throughout the process of succession, while the number with null associations was greatly increased. The same trend existed among the dominant and companion species. The results indicate that the communities are developing towards a stable stage where the woody species coexist in harmony. 2) In the early-established and later invading species, all positive associations were not significant. Compared with positive and null associations, fewer negative associations were found. This implies that these species are inclined to coexist independently through portioning of resources. 3) Among the later invading species, positive associations were significant and no negative associations were found which suggest that these species have similar adaptive ability in the habitat and occupied overlapping niches in the community.

Based on an investigation on gaps and non-gap stands of the Maolan National Karst Forest Nature Reserve, Guizhou Province, quantitative characteristics and dynamic changes of seed rain and seed banks in gaps were analyzed. The results show that the total amount of seed rain was 117.4 ± 32.6 seeds/m² during the period of observation. The number of immature seeds was 56.3 ± 10.3 seeds/m², that of mature damaged seeds was 15.7 ± 4.7 seeds/m², and the number of mature germinated seeds was 45.4 ± 8.2 seeds/m². It is suggested that the seed number is rich for gap regeneration. Seed rain in gaps has spatial and temporal heterogeneities which deeply affect regeneration patterns of gap plants. Along a gradient from the gap center to a non-gap stand, seed density in the litter layer, the number of species, and the Shannon-Wiener diversity index were gradually reduced, but these indices increased in the soil. The seed density in the gap center was 2415 ± 639 seeds/m², near the gap center was 2218 ± 421 seeds/m² and at the gap border area 1815 ± 311 seeds/m². This shows that plants in gaps have good latent regeneration potential. In both gaps and non-gap stands, the Jaccard similarity index of seed in litter layer was the largest, second largest at 5–10 cm soil depth, and the least at the 0–5 cm soil layer the index. The Jaccard index between the soil seed bank and the present plant community was large in the litter layer, but decreased with soil depth both in gaps and non-gap stands. The results show that soil seed banks are the main source of gap regeneration in the karst forests of Maolan and contribute significantly to gap regeneration.

Leaching of major ions from acid precipitation in a subtropical forest was examined based on an experiment in four sample sites in Shaoshan City, Hunan Province, China, from January 2001 to June 2002. Results clearly show that when rain passed through the canopy, pH increased and the evidence of ion uptake was presented for SO₄^2-, NO₃^-, Mg²⁺ and NH₄⁺ ions, especially of NH₄⁺ and NO₃^-. The percentages of dissolved SO₄^2-, Ca²⁺ and Mg²⁺ show a decreasing trend with increasing rainfall. Percentages of leaching Ca²⁺, K⁺ and Cl^- ions show an increasing trend as a function of increased pH values. The forest canopy in Shaoshan City has a strong effect on the uptake of SO₄^2- and NO₃^- ions under acid rain conditions. The decreasing order of ions leaching in the forest canopy is as follows: K⁺ > Ca²⁺ > Cl^- > Mg²⁺ > SO₄^2- > NO₃^- > NH₄⁺ > Na⁺.

The spatial distribution characteristics of plant communities in a wetland-dry grassland ecosystem in arid and semi-arid regions in northwestern China and the main factors affecting the distribution pattern were studied. The Siertan wetland in the Haba Lake Nature Reserve was the study area. Four transect lines, each about 1 km long, were set up in the growing season along the biotope gradient in four directions: east, northeast, west and northwest. Attributes measured include frequency of occurrence, height, density, coverage, biomass and environmental soil factors. The data were analyzed using a two-way indicative species analysis (TWINSPAN), detrended correspondence analysis (DCA) and Spearman correlation coefficients. On this basis, the vegetation in the Siertan wetland was classified into three types and 14 associations. The first vegetation type was halophytic marsh vegetation, mainly distributed in the wetland zone. This type largely contains halophyte and hygric plants and its representative association is Phragmites communis + Kalidium foliatum. The second type was meadow vegetation, distributed in the ecotone. The meadow vegetation species are mainly mesophytic and their representative association is Nitraria tangutorum + Pennisetum flaccidum. The third vegetation type is grassland vegetation, which mainly consists of mesophytic and xerophytes, distributed in the arid grassland zone. The representative association of the third vegetation type is the association of Anaeurolepidium secalium + Saussurea runcinata. Correlation analysis between the axes of DCA and environmental soil factors shows that soil moisture content, organic matter, soil salt content and total nitrogen are the main environmental factors affecting the pattern of vegetation distribution along the biotope gradients from wetland to arid grassland. Other factors affecting the vegetation are microtopography and grazing pressure. Additionally, we have reported modifications and improvements to the importance value methodology.

Land use plays a much more important role than other factors, such as climate, soil properties, topographic features, vegetation coverage, human activities and others, in affecting soil erosion and sediment discharge. In order to understand the effects of changes in land use on sediment discharge and to provide a theoretical basis for land use planning, management and ecological restoration, we used the controlled Qiaozidong watershed and the uncontrolled Qiaozixi watershed in the third sub-region of the Loess Plateau as examples and analyzed the effects of land use and land cover on the discharge of sediments. The results show that the impact of land use and land cover on the annual amount of sediment discharge is significant. Compared with the uncontrolled watershed during similar periods, the amount of sediment discharged from the controlled watershed was reduced by 44%, 75% and 86%, respectively, in wet, normal and dry years. In the controlled watershed, compared with the period from 1986 to 1994, the amount of sediments discharged was less during the period from 1995 to 2004. The impact of land use and land cover on sediment discharge demonstrated characteristics of seasonal fluctuation. The effects of sediment reduction in the controlled watershed were greater than those in the uncontrolled watershed in May and September. In the controlled watershed, the reduction effect coincided with the distribution of rainfall. The amount of discharged flood sediments is closely correlated with rainfall, rainfall intensity in a 60 min period and the volume of flood. The rainstorm-runoff process and the rainstorm-sediment discharge process demonstrate that land cover has a strong regulatory and control function in the flood process and sediment discharge in rainstorms. For the controlled watershed, given the same precipitation frequency distribution, the average amount of sediment discharged during the land use period from 1995 to 2004 was less than that during the earlier land use period from 1986 to 1994 under every recurrent period.

Vegetation fluctuation is an important part of vegetation dynamics. The characteristics of vegetation fluctuation are quite different between grassland and forest communities. By inferring from ecologic and statistical information, grassland vegetation fluctuation and its fluctuation ratio were defined, and an equation for the fluctuation ratio was established for the first time with the fluctuation coefficients specified via expert weighting method. The quantitative grassland vegetation fluctuation and its fluctuation ratio between the years 2002 and 2006 in Yanchi County, Ningxia, northwestern China, in the northern farming-pastoral ecotones, were studied. Results showed that the largest positive fluctuation ratio of 0.685, implying the best vegetation growth in recent years occurred in 2003, while the most negative fluctuation ratio of -1.098, i.e., the worst vegetation growth during this period, occurred in 2005.

To better understand the distribution of soil microorganisms in Populus euphratica forests in Xinjiang, northwestern China, we studied and compared the populations and numbers of bacteria, fungi and actinomycetes in the soil at four different age stages of natural P. euphratica forests, i.e., juvenile forests, middle-aged forests, over-mature forests and degraded forests. Results showed that there were clear differences in the amount of microorganism biomass and composition rates across the four forest stages. Dominant and special microorganisms were present in each of the four different soil layers. The vertical distribution showed that the microorganism biomass decreased with increasing soil depth. The population of microorganisms was the lowest at 31–40 cm of soil depth. The microorganisms consisted of bacteria, actinomycetes, as well as fungi. Bacteria were the chief component of microorganisms and were widely distributed, but fungi were scarce in some soil layers. Aspergillus was the dominant genus among the 11 genera of fungi isolated from the soil in different age stages of P. euphratica forests.

In this paper, the basic principle of chromosome walking is presented and we used an actin gene of radiata pine (Pinus radiata) as an example to conduct upstream and downstream chromosome walking for EST sequences. The full genomic sequence (2154 bp) of the actin gene, including promoters 5′ UTR, CDS and 3′ UTR, was identified by chromosome walking. PCR amplification and DNA band sequencing from 200 unrelated radiata pine trees revealed a total of 21 SNPs for the actin gene, three in the promoter region, 15 in CDS and 4 in 3′ UTR. The results of this experiment provide a technical framework for SNPs discovery in none coding regions of candidate genes.

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Responses of the photosynthetic characteristics to variation in CO₂ concentration and temperature of Ginkgo biloba, Eucommia ulmoides, Magnolia denudata and Tilia japonica were measured during the peak growing season. The results show that the ambient CO₂ concentration could not meet the requirements for photosynthesis of these four species. The optimal temperatures for photosynthesis were lower than the average daytime air temperature. Hence, the photosynthesis of these four species was restricted by the low CO₂ concentration and high daytime air temperature at the time of measurement. Marked enhancements in the net photosynthetic rate were found in all four species when the CO₂ concentration was doubled. When the dependency on CO₂ and temperature were examined simultaneously, it was seen that for increased CO₂ concentrations there was a shift in the optimum temperature for M. denudata and T. japonica towards higher temperatures. Due to their independence on CO₂ concentrations, this trend could not be found in the G. biloba and E. ulmoides data sets. The stomatal conductance (G_s) was sensitive to a vapor pressure deficit (VPD) which in turn was sensitive to temperature. An increase in temperature would cause the VPD to increase and plants might be assumed to react by reducing their stomatal apertures. The effect on stomatal resistance would be most significant at high temperatures. The restriction to stomatal conductance for these four species would increase if CO₂ concentrations were elevated at the same temperature.

Compared with the traditional techniques of forest fire detection, a wireless sensor network paradigm based on a ZigBee technique was proposed. The proposed technique is in real time, given the exigencies of forest fires. The architecture of a wireless sensor network for forest fire detection is described. The hardware circuitry of the network node is designed based on a CC2430 chip. The process of data transmission is discussed in detail. Environmental parameters such as temperature and humidity in the forest region can be monitored in real time. From the information collected by the system, decisions for fire fighting or fire prevention can be made more quickly by the relevant government departments.

In order to reduce the density of wood-based composites without causing a deterioration of their mechanical properties, we studied the process of manufacturing wood-based composites. A combination of polymer foaming technology and flat hot-pressing technology was used. The microscopic structure of the various wood-based composites was analyzed with a scanning electron microscope (SEM). Modulus of rupture (MOR), modulus of elasticity (MOE), impact strength, and thickness expansion rate of water sorption (TS) were all measured. The results showed that fibers loosely interweave, and fibers had been connected by micropore. They also showed that spaces between fibers had big micropore structure. MOR, MOE and impact strength were the highest among three levels of ratio. When the total content of resin and foaming agent were 20% by weight, TS was higher. A hot-pressing temperature of 120°C was optimal. At the low temperatures of 80°C, the foaming process was uncompleted. At a higher temperature, micropores burst at a certain pressure. Based on the variance analysis and maximum difference analysis, a significance test shows that the optimum conditions for the total content of resin and foaming agent is 20% by weight, with a hot pressing temperature of 120°C for 15 min. Under these conditions, the properties of wood-based foaming composites all achieved the industry standard.