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Artificial Shiro formation of Tricholoma matsutake |
| LUN Zhiming1, LI Yuhua2, XING Shutang2, Vaario Lumin2 |
| 1.Institute of Flower Biotechnology, Northeast Forestry University, Harbin 150040, China; Department of Green Agriculture, Heilongjiang Professional College of Agricultural Economy, Mudanjiang 157041, China; 2.Institute of Flower Biotechnology, Northeast Forestry University, Harbin 150040, China |
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Abstract One type of soil collected from Maoer-shan in Heilongjiang Province, China was selected to induce hyphal growth of Tricholoma matsutake by a soil screening experiment. It was confirmed that hyphal growth of all the tested T. matsutake isolates was significantly stimulated in soil by supplemented with 0.5%~2.0% olive oil. The aggregation of hyphae and soil resembled natural Shiro. The biomass of hyphae in the soil increases with increasing olive oil concentrations. Moreover, seedlings of Pinus densiflora grew well in the soil containing 0.5%~1.0% olive oil and were also successfully infected by T. matsutake isolate A in the soil containing 1.0% olive oil. This study established a culture system of artificial Shiro formation and also provided a premise for formulation of culture substratum for fruit body formation of T. matsutake.
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Issue Date: 05 September 2006
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