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The production-influencing factors of extracellular polysaccharide (EPS) from a strain of lactic acid bacteria and EPS extraction |
| CHEN Ying1, SUN Liping2, ZENG Yong2, WANG Lei3, AN Liguo3 |
| 1.College of Chemistry & Biology, Yantai University, Yantai 264005, China; College of Life Science, Shandong Normal University, Jinan 250014, China; 2.College of Chemistry & Biology, Yantai University, Yantai 264005, China; 3.College of Life Science, Shandong Normal University, Jinan 250014, China; |
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Abstract The influencing factors of extracellular polysaccharide (EPS) produced from a strain of lactic acid bacteria (LAB L15) were studied by using the phenol-H2SO4 method. It was demonstrated that the strain produced EPS at the most amount when it was incubated for 40 48 h and when the pH value was 4 under 30?C. Glucose was the most suitable carbon source for LAB-producing EPS. The rough EPS was obtained from L15 culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed of two polysaccharides that were completely different in molecular weight and the amount. The purified EPS was passed through the SephadexG-200 column and it showed that it was a sample purified by thin layer chromatography.
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Issue Date: 05 September 2006
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