Cadmium and copper uptake and accumulation by <italic>Sesbania rostrata</italic> seedling, a N-fixing annual plant: implications for the mechanism of heavy metal tolerance

doi:10.1007/s11515-009-0008-7

Front Biol Chin

2009, Vol. 4

Issue (2) : 200-206 https://doi.org/10.1007/s11515-009-0008-7

RESEARCH ARTICLE

Cadmium and copper uptake and accumulation by Sesbania rostrata seedling, a N-fixing annual plant: implications for the mechanism of heavy metal tolerance

Fuhua CHEN¹, Wei FANG², Zhongyi YANG¹(

), Jiangang YUAN¹

1. School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; 2. Biology Department, Long Island University-Brooklyn, 1 University Plaza, Brooklyn, NY 11201, USA

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Abstract

Sesbania rostrata, an annual tropical legume, has been found to be tolerant to heavy metals, with an unknown mechanism. It is a promising candidate species for revegetation at mine tailings. In this study, sequential extractions with five buffers and strong acids were used to extract various chemical forms of cadmium and copper in S. rostrata, with or without Cd or Cu treatments, so that the mechanisms of tolerance and detoxification could be inferred. Both metals had low transition rates from roots to the aboveground of S. rostrata. The transition ratio of Cd (4.00%) was higher than that of Cu (1.46%). The proportion of NaCl extracted Cd (mostly in protein-binding forms) increased drastically in Cd treated plants from being undetectable in untreated plants. This suggests that Cd induced biochemical processes producing protein-like phytochelatins that served as a major mechanism for the high Cd tolerance of S. rostrata. The case for Cu was quite different, indicating that the mechanism for metal tolerance in S. rostrata is metal-specific. The proportion of water-insoluble Cu (e.g. oxalate and phosphate) in roots increased significantly with Cu treatment, which partially explains the tolerance of S. rostrata to Cu. However, how S. rostrata copes with the high biotic activity of inorganic salts of Cu, which increased in all parts of the plant under Cu stress, is a question for future studies. Sesbania rostrata is among the very few N-fixing plants tolerant to heavy metals. This study provides evidence for the detoxification mechanism of metals in Sesbania rostrata.

Keywords Sesbania rostrata phytoremediation heavy metal tolerance sequential extraction chemical forms

Corresponding Author(s): YANG Zhongyi,Email:adsyzy@mail.sysu.edu.cn

Issue Date: 05 June 2009

Cite this article:

Fuhua CHEN,Wei FANG,Zhongyi YANG, et al. Cadmium and copper uptake and accumulation by Sesbania rostrata seedling, a N-fixing annual plant: implications for the mechanism of heavy metal tolerance[J]. Front Biol Chin, 2009, 4(2): 200-206.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-009-0008-7
https://academic.hep.com.cn/fib/EN/Y2009/V4/I2/200

Tab.1 Extraction buffers for different chemical forms of heavy metals

Tab.2 Concentration and transition of Cd and Cu in roots, stems and leaves of

Fig.1 Concentrations of different Cd and Cu forms in roots, stems and leaves of of the treated groups (a, c) and the control groups (b, d). Each bar represents the average concentration+1SD (standard deviation).

Fig.2 Percentage distribution of different Cd forms in roots, stems and leaves of in the control group and Cd treated group

Fig.3 Percentage distribution of different Cu forms in roots, stems and leaves of in the control group and Cu treated group

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