The effect of ethylene inhibitors (AgNO<sub>3</sub>, AVG), an ethylene- liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid <italic>Cymbidium </italic>(Orchidaceae)

doi:10.1007/s11515-013-1283-x

Front Biol

2013, Vol. 8

Issue (6) : 606-610 https://doi.org/10.1007/s11515-013-1283-x

RESEARCH ARTICLE

The effect of ethylene inhibitors (AgNO₃, AVG), an ethylene- liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid Cymbidium (Orchidaceae)

Jaime A. TEIXEIRA DA SILVA^1,2(

)

1. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan; 2. Present address: P. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan

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Abstract

Protocorm-like bodies (PLBs) or thin cell layers (TCLs) derived from PLBs of hybrid Cymbidium Twilight Moon ‘Day Light’ can induce new or neo-PLBs on Teixeira Cymbidium (TC) medium, which contains 0.1 mg/L α-naphthaleneacetic acid, 0.1 mg/L kinetin, 2 g/L tryptone and 20 g/L sucrose, and is solidified with 8 g/L Bacto agar. This study aimed to assess the response of neo-PLB formation to an ethylene-liberating compound (2-chloroethylphosphonic acid (CEPA)), to two ethylene inhibitors (silver nitrate (AgNO₃) and aminoethoxyvinylglycine (AVG)), and to aeration (made possible by using Milliseal^? or autoclaved filter paper). AgNO₃ at 1 or 2 mg/L in TC medium significantly increased the fresh weight of PLBs while 1 mg/L of AgNO₃ also showed a significant increase in the number of neo-PLB from both half-PLBs and from tTCLs. In contrast, AVG and CEPA inhibited neo-PLB formation. Neo-PLB formation from half-PLB or TCL explants in the presence of aeration resulted in significantly lower neo-PLB weight. The use of AgNO₃ and aeration are alternative means to mass produce neo-PLBs for micropropagation purposes.

Keywords AgNO₃ AVG CEPA PLB Teixeira Cymbidium (TC) medium

Corresponding Author(s): TEIXEIRA DA SILVA Jaime A.,Email:jaimetex@yahoo.com

Issue Date: 01 December 2013

Cite this article:

Jaime A. TEIXEIRA DA SILVA. The effect of ethylene inhibitors (AgNO₃, AVG), an ethylene- liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid Cymbidium (Orchidaceae)[J]. Front Biol, 2013, 8(6): 606-610.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-013-1283-x
https://academic.hep.com.cn/fib/EN/Y2013/V8/I6/606

Fig.1 (A) Protocorm-like body (PLB)-derived transverse thin cell layer (tTCL) (left) or half-PLB (right) forming PLBs 60 days after culture on Teixeira medium No. 1 (TC; ) containing 0.1 mg/L a-naphthaleneacetic acid and 0.1 mg/L kinetin. (B) PLB formation on TC without plant growth regulators but supplemented with 2.0 mg/L AgNO. (C) PLB formation with excessive trichomes on TC without plant growth regulators but supplemented with 2.0 mg/L CEPA. Bars= 1 mm (A, for left and right), 5 mm (B, C). (A) modified from Teixeira da Silva ().

Tab.1 Effect of ethylene inhibitors, an ethylene-liberating compound and aeration on -PLB formation from half-PLBs or PLB tTCLs 60 days after culture of hybrid Twilight Moon ‘Day Light’

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