A functional approach toward xerogel immobilization for encapsulation biocompatibility of <italic>Rhizobium</italic> toward biosensor

doi:10.1007/s11515-013-1286-7

Front Biol

2013, Vol. 8

Issue (6) : 626-631 https://doi.org/10.1007/s11515-013-1286-7

RESEARCH ARTICLE

A functional approach toward xerogel immobilization for encapsulation biocompatibility of Rhizobium toward biosensor

Pooja Arora¹, Sunita Sharma²(

), Sib Krishna Ghoshal³, Neeraj Dilbaghi¹(

), Ashok Chaudhury¹(

)

1. Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India; 2. Light and Matter Physics Group, Raman Research Institute, Bangalore, India; 3. Physics Department, University Teknologi of Malaysia, Malaysia

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Abstract

Sol-gel derived silica has tremendous applications as a biocompatible scaffold for the immobilization of cells. The use of xerogel as a matrix in the blueprint of biosensors is an appealing proposition due to several inimitable characteristics of xerogels, primarily because of their high porous nature, amendable pore size, and exceptionally large internal surface area. Morphological (X-Ray Diffraction and Thermogravimmetric Analysis) and optical (Fourier Transform Infrared and UV-Vis absorption) studies of the silica matrices with entrapped Rhizobial (Rz) structure of the biomaterial has been made. Temporal and concentration dependent studies were conducted for impregnated samples; it showed that the response time for the new biosensor for determining the concentration of Rz is less than 20 min. In this work, first time a novel avenue to create a generic approach for the fabrication of biosensor has been created.

Keywords biosensor Fourier Transform Infrared spectroscopy (FTIR) Rhizobium Thermo Gravimmetric Analysis (TGA) sol-gel xerogel X-Ray Diffraction (XRD)

Corresponding Author(s): Sharma Sunita,Email:sunphotonics@gmail.com; Dilbaghi Neeraj,Email:ndnano@gmail.com; Chaudhury Ashok,Email:ashokchaudhury@hotmail.com

Issue Date: 01 December 2013

Cite this article:

Sunita Sharma,Sib Krishna Ghoshal,Pooja Arora, et al. A functional approach toward xerogel immobilization for encapsulation biocompatibility of Rhizobium toward biosensor[J]. Front Biol, 2013, 8(6): 626-631.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-013-1286-7
https://academic.hep.com.cn/fib/EN/Y2013/V8/I6/626

Fig.1 Schematic diagram of z-scan set up

Fig.2 Visual characteristic of the grown samples (Blank (1), RhB co-doped Rz (2=pre-doping, 3=post-doping))

Fig.3 TGA thermogram of blank sample

Fig.4 Concentration dependent UV-Vis absorption spectra of Rz doped silica matrices

Fig.5 FTIR spectra of dye doped samples (with and without Rz) (a) and the inset of a portion of it (b)

Fig.6 Variation of output intensity with dopant volume

Fig.7 Temporal response of the sample at 0.2 mL of Rz

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