Please wait a minute...
Shopping Cart Email List Chinese
Advanced Search Fig/Tab
Frontiers in Biology

ISSN 1674-7984

ISSN 1674-7992(Online)

CN 11-5892/Q

Featured Articles  |  Most Downloaded  |  Most Reading
Online Submission Subscribe to Our RSS Content Feed
Front. Biol.    2015, Vol. 10 Issue (5) : 473-473    https://doi.org/10.1007/s11515-015-1372-0
ERRATUM
Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology
Lihua Julie Zhu()
Department of Molecular, Cell and Cancer Biology, Program in Bioinformatics and Integrated Biology, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
 Download: PDF(45 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Corresponding Author(s): Lihua Julie Zhu   
Online First Date: 13 October 2015    Issue Date: 30 October 2015
 Cite this article:   
Lihua Julie Zhu. Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology[J]. Front. Biol., 2015, 10(5): 473-473.
 URL:  
https://academic.hep.com.cn/fib/EN/10.1007/s11515-015-1372-0
https://academic.hep.com.cn/fib/EN/Y2015/V10/I5/473
Functions/Limits CRISPRseek1 Zhang Lab2 eCRISP3 Kong Lab4 CRISPR MultiTargeter5 sgRNA Designer6
Off-target analysis Yes with PDMM penalty scoring Yes with PDMM penalty scoring Yes without scoring Yes without scoring Yes without scoring No
gRNA efficacy prediction Yes No No No No Yes
Batch search Yes Yes Yes No No No
Paired configuration Nickase, dCas9-Fok1 and Cas9-DBD fusion (dev) Nickase No No No No
Cas9 type Flexible SpCas9 Only SpCas9 Only SpCas9 Only SpCas9 Only SpCas9 Only
Alternative scoring matrix for efficacy and off-targets Yes No No No No No
Target species Any A specified set A specified set A specified set Human and mouse
Restriction enzyme sites Yes No No No No No
Compare set of sequences Yes No No No Yes No
Predict secondary structure Yes No No Yes No No
Tab.1  
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed