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Frontiers in Biology

ISSN 1674-7984

ISSN 1674-7992(Online)

CN 11-5892/Q

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Front. Biol.    2018, Vol. 13 Issue (1) : 63-69    https://doi.org/10.1007/s11515-018-1478-2
RESEARCH ARTICLE
Synthesis of scl-poly (3-hydroxyalkanoates) by Bacillus cereus found in freshwater, from monosaccharides and disaccharides
Tayyaba Naeem, Naima Khan, Nazia Jamil()
Department of Microbiology and Molecular Genetics, University of the Punjab-Quaid-i-Azam Campus, Lahore-54590, Pakistan
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Abstract

BACKGROUND: Polyhydroxyalkanoates are a good substitute for synthetic plastic because they are highly biocompatible, ecofriendly, and biodegradable. Bacteria in freshwater bodies such as rivers, tube wells, and canals are exposed to alternating high and low concentrations of substrates that induce PHA production.

METHODS: Fresh water samples were collected for isolation of bacterial strains. Screening of PHA in bacterial cells was performed with Sudan and Nile Red staining. Extracted PHA was characterized by FTIR.

RESULTS: In this study, nine bacterial isolates were selected for PHA production on the basis of phenotypic screening. Their ability to accumulate PHAs was determined using different monosaccharides and disaccharides. Two bacterial isolates Bacillus cereus T1 (KY746353) and Bacillus cereus R3 (KY746354) produced PHAs. Optimal growth of the bacterial strain (T1) was observed in the presence of glucose, followed by maximum production of PHAs (63% PHAs) during the logarithmic phase of growth. B. cereus R3 (KY746354) accumulated 60% PHAs by dry cell weight.

CONCLUSION: PHA accumulation was relatively less with fructose, but both strains showed increased production (up to 50%) with sucrose. The polymer produced was characterized by Fourier-transform infrared spectroscopy (FTIR), which showed that the compound contains short-chain PHAs.
Keywords ecofriendly      biocompatible      scl-poly (3-hydroxyalkanoates)      Bacillus cereus     
Corresponding Author(s): Nazia Jamil   
Online First Date: 15 March 2018    Issue Date: 26 March 2018
 Cite this article:   
Tayyaba Naeem,Naima Khan,Nazia Jamil. Synthesis of scl-poly (3-hydroxyalkanoates) by Bacillus cereus found in freshwater, from monosaccharides and disaccharides[J]. Front. Biol., 2018, 13(1): 63-69.
 URL:  
https://academic.hep.com.cn/fib/EN/10.1007/s11515-018-1478-2
https://academic.hep.com.cn/fib/EN/Y2018/V13/I1/63
Fig.1  T1, R3, and T3 show fluorescence in plates containing PDA with glucose source during Nile Red screening
Fig.2  Light microscopy analysis of the black granules produced by PHA-producing bacteria in the presence of glucose, stained using Sudan staining
Fig.3  Phylogenetic relationship between Bacillus strains and the organism used in this study, namely, T1 (KY746353)
Fig.4  FTIR spectrum of PHA produced by Bacillus cereus T1 (KY746353) in the control medium supplemented with glucose
Fig.5  Optical density of T1 and R3 strains at 600nm using different carbon sources. S, G and F are the initials of sucrose, glucose and fructose.
Fig.6  Comparative analysis of the PHA (%) and biomass (g/L) produced by T1 and R3 strains, using different carbon sources
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