Isolation and characterization of marine biofilm forming bacteria from a ship’s hull

doi:10.1007/s11515-018-1496-0

Front. Biol.

2018, Vol. 13

Issue (3) : 208-214 https://doi.org/10.1007/s11515-018-1496-0

RESEARCH ARTICLE

Isolation and characterization of marine biofilm forming bacteria from a ship’s hull

Kavitha S¹, Vimala Raghavan²(

)

¹. School of Biosciences and Technology, Vellore Institute of Technology, Vellore-632-014, Tamil Nadu, India
². Centre for Nanotechnology Research, Vellore Institute of Technology, Vellore-632 014, Tamil Nadu, India

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Abstract

BACKGROUND: Diverse aquatic microorganisms are capable of colonizing living and non-living surfaces leading to the formation of biofilms. Commonly visualized as a slimy layer, these biofilms are filled with hundreds of other microorganisms compared to free living planktonic cells. Microbial surface colonization and surface-associated metabolic activities also exert several macroscale deleterious effects, including biofouling, biocorrosion and the persistence and transmission of harmful or pathogenic microorganisms and virulence determinants. The present study deals with the isolation and screening of marine bacteria for biofilm formation. The screened isolates were characterized and identified as Psychrobacter celer, Psychrobacter alimentarius and Kocuria rhizophila by 16S rRNA sequencing.

METHODS: Biofilm forming bacteria were isolated by spread plate technique and subjected to screening by microtiter plate assay. The potent biofilm formers were identified by molecular characterization using 16S rRNA gene sequencing.

RESULTS: Twelve bacterial isolates were obtained by pour plate technique and subjected to biofilm assay. Among the 12 isolates three isolates which showed maximum biofilm formation were subjected to molecular characterizationby 16S rRNA gene sequencing method. The isolates were identified as Psychrobacter celer, Psychrobacter alimentarius and Kocuria rhizophila. The EPS produced by the three biofilm forming bacteria was extracted and the protein and carbohydrate content determined.

CONCLUSION: Among the isolates screened, isolate 8 (Kocuria rhizophila) produced maximum protein and carbohydrate which was also in accordance with the results of microtiter plate assay.

Keywords biofilm MTP assay extracellular polymeric substances 16S rRNA gene sequencing Psychrobacter Kocuria

Corresponding Author(s): Vimala Raghavan

Online First Date: 12 July 2018 Issue Date: 31 July 2018

Cite this article:

Kavitha S,Vimala Raghavan. Isolation and characterization of marine biofilm forming bacteria from a ship’s hull[J]. Front. Biol., 2018, 13(3): 208-214.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-018-1496-0
https://academic.hep.com.cn/fib/EN/Y2018/V13/I3/208

Fig.1 Microtiter plate assay of Isolate 3, Isolate 4 and Isolate 8.

Fig.2 Screening of biofilm forming bacteria by Micro Titer Plate assay.

Tab.1 Morphological and biochemical characterization of biofilm forming bacteria

Fig.3 Phylogenetic analysis of biofilm forming bacteria. (a) Psychrobacter celer, (b). Psychrobacter alimentarius, (c) Koucuria rhizophila.

Fig.4 Scanning electron microscopic images (a)P.celer, (b) P. alimentarius and (c)K. rhizophila.

Tab.2 Chemical analysis of extracellular polymeric matrix produced by biofilm forming bacteria

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