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Frontiers in Biology

ISSN 1674-7984

ISSN 1674-7992(Online)

CN 11-5892/Q

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Front. Biol.    2018, Vol. 13 Issue (5) : 358-365    https://doi.org/10.1007/s11515-018-1514-2
RESEARCH ARTICLE
Investigation of the relationship between cell surface hydrophobicity and demulsifying capability of biodemulsifier-producing bacteria
Hoda Sabati1, Hossein Motamedi1,2()
1. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2. Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran
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Abstract

BACKGROUND: Cell surface hydrophobicity (CSH) is one of the key physicochemical features of biodemulsifier-producing bacteria that influence their demulsification capability maintenance in petroleum contaminated environments.

METHODS: In present study, biodemulsifier-producing bacteria were isolated from petroleum contaminated environments using different isolation media and the correlation between their CSH and demulsifying ability was investigated. The demulsifying ability of isolates was measured through demulsification tests on water in kerosene emulsions. The microbial adhesion to the hydrocarbon (MATH) assay was used to denote their CSH.

RESULTS: The evaluation of CSH showed that majority of biodemulsifier producing bacteria have high CSH which indicating a positive correlation between CSH and demulsifying capability.

CONCLUSIONS: According to these results it can be concluded that CSH can be used as an indicator for assessment of biodemulsifier-producing bacteria and screening of new isolates for their biodemulsifier production.
Keywords bacterial adhesion      biodemulsifier      biodemulsifier-producing bacteria      demulsification      hydrophobicity      MATH assay     
Corresponding Author(s): Hossein Motamedi   
Online First Date: 13 September 2018    Issue Date: 25 October 2018
 Cite this article:   
Hoda Sabati,Hossein Motamedi. Investigation of the relationship between cell surface hydrophobicity and demulsifying capability of biodemulsifier-producing bacteria[J]. Front. Biol., 2018, 13(5): 358-365.
 URL:  
https://academic.hep.com.cn/fib/EN/10.1007/s11515-018-1514-2
https://academic.hep.com.cn/fib/EN/Y2018/V13/I5/358
Property Unit NIORDC a standard
Freezing point (max) °C -47
Density at 15°C (max.) kg/m3 775-830
Distillation recovered at 185°C (max.) Vol% 50
Distillation FBP (max.) °C 275
Total aromatics content (max) Vol% 25
Smoke point (min) mm 25
Flash point (min) °C 43
Tab.1  Properties of kerosene used for preparing the emulsion model
Code of isolated source Demulsification ratio (%) MATH (%)
1 Crude oil 47.85±3 70.19±0
2 Crude oil 26.43±2 78.71±1
3 Crude oil 95.71±1 83.02±2
4 Crude oil 64.29±2 81.36±3
5 Drainage 49.66±0.4 78.52±0
6 Drainage 49±0 75.80±0
7 Drainage 97.14±2 85.96±2
8 Drainage 42.86±2 77.24±1
9 Crude oil contaminated soil 25±3 64.43±0
10 Crude oil contaminated soil 28.57±5 73.76±1
11 Crude oil contaminated soil 27.14±1 62.22±0
12 Crude oil contaminated soil 51.57±1 67.82±4
13 Crude oil sludge 30.71±2 69.71±1
14 Crude oil sludge 30.71±2 74.05±2
15 Crude oil sludge 50.43±2 69.27±1
16 Crude oil sludge 33.56±2 75.23±1
17 Crude oil sludge 28.57±0 66.38±0
18 Crude oil precipitate 32.14±6 76.11±2
19 Crude oil precipitate 92.85±3 76.11±1
20 Crude oil precipitate 71.43±1 72.99±0
21 Crude oil precipitate 38.57±2 65.38±2
22 Crude oil precipitate 85.71±3 72.24±1
23 Crude oil precipitate 50.14±1 69.58±1
24 Crude oil precipitate 50.28±1 69.89±2
Tab.2  Demulsification ratio and MATH percentage of the isolated strains from environmental samples of the refinery a
Fig.1  Demulsification capability and cell surface hydrophobicity of the demulsifying bacteria with taking into account the standard deviation of the experiments. The cell surface hydrophobicity of the isolated bacterial strains was determined by evaluation of their adhesion to the hydrocarbons according to MATH assay.
Fig.2  Linear relationship between the demulsification capability and cell surface hydrophobicity of the demulsifying bacteria. The positive relationship between these two variables is quite evident.
Fig.3  Correlation between the demulsification ratio and MATH of the demulsifying bacteria. The demulsification ratio was plotted as a function of the MATH. This figure showed that the demulsification ability of the demulsifying strains was largely dependent on their cell surface hydrophobicity.
Fig.4  The adsorption capability of demulsifying cells in the oil phase (left) and the control (right). The control contained 3 ml of PBS and 3 ml of kerosene. The production of biodemulsifiers by demulsifying bacteria facilitated the dispersion of them into the oil phase.
Fig.5  Relationship between the demulsification capability and cell surface hydrophobicity of the demulsifying bacteria with≥50 percentage of demulsification ratio. Best demulsification performance have been seen in strains which have high cell surface hydrophobicity.
Fig.6  Relationship between the demulsification capability and cell surface hydrophobicity of the demulsifying bacteria with ?50 percentage of demulsification ratio. High levels of cell surface hydrophobicity do not always mean high demulsification.
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