Alteration of heat shock protein 20 expression in preeclamptic patients and its effect in vascular and coagulation function

doi:10.1007/s11684-017-0576-x

Front. Med.

2018, Vol. 12

Issue (5) : 542-549 https://doi.org/10.1007/s11684-017-0576-x

RESEARCH ARTICLE

Alteration of heat shock protein 20 expression in preeclamptic patients and its effect in vascular and coagulation function

Fanfan Li¹, Mengzhou He¹, Meitao Yang¹, Yao Fan¹, Yun Chen², Xi Xia², Yin Xie¹, Dongrui Deng¹(

)

¹. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
². Ultrosonic Department, Peking University Shenzhen Hospital, Shenzhen 518035, China

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Abstract

Preeclampsia (PE) is a pregnancy-specific, multi-system disorder and the leading cause of maternal and perinatal morbidity and mortality in obstetrics worldwide. Excessive vasoconstriction and dysregulated coagulation function are closely associated with PE. Heat shock protein 20 (HSP20) is ubiquitously expressed under normal physiological conditions and has important roles in vascular dilatation and suppression of platelet aggregation. However, the role of HSP20 in the pathogenesis of PE remains unclear. In this study, we collected chorionic plate resistance arteries (CPAs) and serum from 118 healthy pregnant women and 80 women with PE and detected the levels of HSP20 and its phosphorylated form. Both HSP20 and phosphorylated HSP20 were downregulated in CPAs from women with PE. Comparison of the vasodilative ability of CPAs from the two groups showed impaired relaxation responses to acetyl choline in preeclamptic vessels. In addition to the reduced HSP20 in serum from women with PE, the platelet distribution width and mean platelet volume were also decreased, and the activated partial thromboplastin time and thromboplastin time were elevated. With regard to the vital roles of HSP20 in mediating vasorelaxation and coagulation function, the decreased HSP20 might contribute to the pathogenesis of PE.

Keywords preeclampsia heat shock protein 20 vascular relaxation coagulation-fibrinolytic system

Corresponding Author(s): Dongrui Deng

Just Accepted Date: 12 December 2017 Online First Date: 08 March 2018 Issue Date: 29 September 2018

Cite this article:

Fanfan Li,Mengzhou He,Meitao Yang, et al. Alteration of heat shock protein 20 expression in preeclamptic patients and its effect in vascular and coagulation function[J]. Front. Med., 2018, 12(5): 542-549.

URL:

https://academic.hep.com.cn/fmd/EN/10.1007/s11684-017-0576-x
https://academic.hep.com.cn/fmd/EN/Y2018/V12/I5/542

Tab.1 Baseline characteristics of normal pregnant women and patients with preeclampsia (PE)

Fig.1 Location of HSP20 and pHSP20 in placental arteries. Placental arteries were immunohistochemically assessed (400×; A−F) with negative controls (G). Blue spots represent nuclei. Arrows indicate antibody stain. HSP20 and pHSP20 were located in the cytoplasm and on the cell surface of smooth muscle. The AOD represents the expression intensities of HSP20 and pHSP20 (H, I). *P<0.05.

Fig.2 Expressions of HSP20 and pHSP20 in placental arteries. (A, B) Representative photomicrographs of Western blot for HSP20 and pHSP20, respectively. (C) Relative quantification of HSP20 protein expression. (D) Relative quantification of pHSP20 protein expression. *P<0.05.

Fig.3 Relaxation of placental arteries after ACh. Preconstricted placental arteries from mild and severe PE had significantly poorer responses to ACh than those from normal pregnancy from 10⁻⁹ to 10⁻⁵mol/L.*P<0.05, **P<0.01.

Fig.4 Serum HSP20 and coagulation factors. (A−I) Comparisons of coagulation factors, platelet-related indices, and HSP20 among normal pregnancy and mild and severe PE during the third trimester of pregnancy. *P<0.05, **P<0.01.

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