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Frontiers of Optoelectronics

ISSN 2095-2759

ISSN 2095-2767(Online)

CN 10-1029/TN

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Front. Optoelectron.    2017, Vol. 10 Issue (3) : 292-298    https://doi.org/10.1007/s12200-017-0720-x
RESEARCH ARTICLE
Excitation-emission matrices (EEMs) of colorectal tumors – tool for spectroscopic diagnostics of gastrointestinal neoplasia
E. BORISOVA1(), Ts. GENOVA1, O. SEMYACHKINA-GLUSHKOVSKAYA2, N. PENKOV3, I. TERZIEV3, B. VLADIMIROV3
1. Institute of Electronics, Bulgarian Academy of Sciences, Tsarigradsko Chaussee 72, Sofia 1784, Bulgaria
2. Saratov State University, Astrakhanskaya Str. 83, Saratov 410012, Russia
3. Tsaritsa Yoanna – ISUL University Hospital, 8, Byalo More Str., Sofia 1572, Bulgaria
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Abstract

The autofluorescence spectroscopy of biological tissues is a powerful tool for non-invasive detection of tissue pathologies and evaluation of any biochemical and morphological changes arising during the lesions’ growth. To obtain a full picture of the whole set of endogenous fluorophores appearing in the gastrointestinal (GI) tumors investigated, the technique of excitation-emission matrix (EEM) development was applied in a broad spectral region, covering the ultraviolet and visible spectral ranges. We could thus address a set of diagnostically-important chromophores and their alterations during tumor development, namely, collagen, elastin, nicotinamide adenine dinucleotide (NADH), flavins, porphyrins, while hemoglobin’s absorption influence on the spectra obtained could be evaluated as well. Comparisons are presented between EEM data of normal mucosae, benign polyps and malignant carcinoma, and the origins are determined of the fluorescence signals forming these matrices.
Keywords autofluorescence spectroscopy      excitation-emission matrix (EEM)      colon carcinoma      gastrointestinal (GI) tumours     
Corresponding Author(s): E. BORISOVA   
Just Accepted Date: 30 June 2017   Online First Date: 09 August 2017    Issue Date: 26 September 2017
 Cite this article:   
E. BORISOVA,Ts. GENOVA,O. SEMYACHKINA-GLUSHKOVSKAYA, et al. Excitation-emission matrices (EEMs) of colorectal tumors – tool for spectroscopic diagnostics of gastrointestinal neoplasia[J]. Front. Optoelectron., 2017, 10(3): 292-298.
 URL:  
https://academic.hep.com.cn/foe/EN/10.1007/s12200-017-0720-x
https://academic.hep.com.cn/foe/EN/Y2017/V10/I3/292
Fig.1  Excitation-emission matrices of (a) normal mucosa, (b) benign colon polyp, (c) colon carcinoma and (d) rectum carcinoma for the 280−440 nm excitation wavelength range and emission detected in the 300−800 nm wavelength range. The EEM data presented are the resulting averaged spectrum of all tissue samples of a given type detectedex vivo
Fig.2  Autofluorescence spectra of (a) normal mucosa, (b) benign colon polyp, (c) colon carcinoma and (d) rectum carcinoma for different excitation wavelengths applied in the 280−440 nm region, normalized with respect to the integral intensity (spectral curve area)
Fig.3  Autofluorescence spectra of normal, benign and malignant gastrointestinal tissues using excitation at 420 nm. The spectra are normalized with respect to the integral intensity of the fluorescence emission
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