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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

Postal Subscription Code 80-974

2018 Impact Factor: 1.701

Front. Mater. Sci.    2008, Vol. 2 Issue (2) : 162-166    https://doi.org/10.1007/s11706-008-0027-3
Daily oscillation of gene expression associated with nacreous layer formation
MIYAZAKI Yoko, USUI Tomomi, KAJIKAWA Aya, HISHIYAMA Hajime, MATSUZAWA Norifumi, NISHIDA Takuma, MACHII Akira, SAMATA Tetsuro
Laboratory for Cell Biology, Department of Environmental Health, Azabu University, 1-17-71 Fuchinobe, Sagamihara-shi
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Abstract Three major organic matrix components, nacrein, MSI60 and N16 have been reported from the nacreous layer of Japanese pearl oyster, Pinctada fucata. Though several in vitro experiments have been carried out to elucidate the functions of these molecules details have not yet been clarified. In this report, we tempt to clarify the gene expression levels encoding the above three proteins between samples of 1) summer and winter seasons and 2) ocean and aquarium environments by using real-time polymerase chain reaction (PCR).It was confirmed that the biomineralization process of P. fucata is mainly influenced by the circatidal rhythm of the ocean environment. The gene expressions coding for N16 and MSI60 increased at the time of high tide, while that of nacrein increased at the time of low tide. The similar tendency observed in N16 and MSI60 showed the possibility that both components are secreted simultaneously, supporting a hypothesis that N16 forms cross-linkage with MSI60 to form the membrane. The expressions of MSI60, N16 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were remarkable in winter season, while no variation was found in the expression level of the nacrein gene in summer and winter season.The study is the first attempt regarding the seasonal and circadian rhythms observed on gene expressions incorporated into molluscan shell formation. The results will give a new insight into the relationship between molluscan physiology and the mechanism of shell formation.
Issue Date: 05 June 2008
 Cite this article:   
USUI Tomomi,MIYAZAKI Yoko,MACHII Akira, et al. Daily oscillation of gene expression associated with nacreous layer formation[J]. Front. Mater. Sci., 2008, 2(2): 162-166.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-008-0027-3
https://academic.hep.com.cn/foms/EN/Y2008/V2/I2/162
1 Crenshaw M A Thesoluble matrix from Mercenaria mercenaria shellBiomineralization 1972 6611
2 Weiner S Asparaticacid-rich proteins: major components of the soluble organic matrixmollusk shellsCalcified Tissue International 1979 29163167.
doi:10.1007/BF02408072
3 Cariolou M A Morse D E Purification and characterizationof calcium-binding conchiolin shell peptides from the mollusk, Haliotis rufescens, as a function of developmentJournal of Comparative Physiology B: Biochemical,Systems, and Environmental Physiology 1988 157717729.
doi:10.1007/BF00691002
4 Miyamoto H Miyashita T Okushima M et al.A carbonic anhydrase from the nacreous layer inoyster pearlsProceedings of the NationalAcademy of Sciences of the United States of America 1996 9396579660.
doi:10.1073/pnas.93.18.9657
5 Sudo S Fujikawa T Nagakura T et al.Structure of mollusc shell framework proteinsNature 1997 387563564.
doi:10.1038/42391
6 Samata T Hayashi N Kono M et al.A new matrix protein family related to the nacreouslayer formation of Pinctada fucataFEBS Letters 1999 462225229.
doi:10.1016/S0014‐5793(99)01387‐3
7 Suzuki M Murayama E Inoue H et al.Characterization of Prismalin-14, a novel matrixprotein from the prismatic layer of the Japanese peal oyster (Pinctada fucata)Biochemical Journal 2004 382205213.
doi:10.1042/BJ20040319
8 Tsukamoto D Sarashina I Endo K Structure and expression of an unusual acidic matrix proteinof pearl oyster shellsBiochemical and BiophysicalResearch Communications 2004 320(4)11751180.
doi:10.1016/j.bbrc.2004.06.072
9 Yano M Nagai K Morimoto K et al.Shematrin: a family of glycine-rich structural proteinsin the shell of the pearl oyster PinctadafucataComparative Biochemistryand Physiology B: Biochemistry and Molecular Biology 2006 144(2)254262.
doi:10.1016/j.cbpb.2006.03.004
10 Wada K Theinfluence of the life-activity upon pearl formation in the pearl cultureof Pinctada martensii (Dunker)I. On the pearls produced under abnormal conditions of their healthBulletin of the National Pearl Research Laboratory 1959 5381394
11 Wada K Theinfluence of the life-activity upon pearl formation in the pearl cultureof Pinctada martensii (Dunker)II. The seasonal changes of crystal growth and luster of the pearlBulletin of the National Pearl Research Laboratory 1961 6586606
12 Wada K Relationsbetween growth of pearls and water temperatureBulletin of the Japanese Society of Fisheries Oceanography 1969 299301
13 Wada K Nucleationand growth of aragonite crystals in the nacre of some bivalve mollusksBiomineralization 1972 6141150
14 Wada K Growthof CaCO3 crystals in bivalve shell mineralizationJapanese Journal of Crystal Growth 1985 125770
15 Moura G Almeida M J Machado M J C et al.Annual variation of some inorganic elements presentin the biological fluids of Anodonta cygnea (L.) and possible effects on its calcification cycleBiomineralization (BIOM2001) 2003a 123128
16 Moura G Almeida M J Machado M J et al.The action of environmental acidosis on the calcificationprocess of Anodonta cygnea (L.)Biomineralization (BIOM2001) 2003b 178182
17 Takeuchi T Endo K Biphasic and dually coordinatedexpression of the genes encoding major shell matrix proteins in thepearl oyster Pinctada fucataMarine Biotechnology 2006 8(1)5261.
doi:10.1007/s10126‐005‐5037‐x
18 Saigusa M Biologicalrhythms in the marine environment: a perspectiveAquabiology 2002 24473474
19 Saigusa M A widevariety of patterns in the tidal rhythm, and the timing systems ofthe circatidal rhythmAquabiology 2002 24475483
20 Kayanne H Suzuki A Saito H Diurnal changes in the partial pressure of carbon dioxidein coral reef waterScience 1995 269(5221)214216.
doi:10.1126/science.269.5221.214
21 Kayanne H Coralreefs and carbon dioxideScience 1996 271(5253)12991300.
doi:10.1126/science.271.5253.1299
22 Samata T Recentadvances in studies on nacreous layer biomineralization. Molecularand cellular aspects. Thalassas 2004 20(1)2746
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