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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

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Front. Mater. Sci.    2023, Vol. 17 Issue (1) : 230627    https://doi.org/10.1007/s11706-023-0627-y
RESEARCH ARTICLE
Carbon dots based on targeting unit inheritance strategy for Golgi apparatus-targeting imaging
Yingying Wei1(), Yuduan Gao2,3(), Lin Chen4, Qiang Li1, Jinglei Du1, Dongming Wang5, Fanggang Ren6, Xuguang Liu7, Yongzhen Yang4
1. Interventional Treatment Department, Second Hospital of Shanxi Medical University (Second Clinical Medical College of Shanxi Medical University), Taiyuan 030001, China
2. Ophthalmology Department, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China
3. Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
4. Key Laboratory of Interface Science and Engineering in Advanced Materials (Ministry of Education), Taiyuan University of Technology, Taiyuan 030024, China
5. Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases of Shanxi Province, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
6. Orthopedics Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
7. Institute of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
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Abstract

The Golgi apparatus is one of the important organelles, where the final processing and packaging of cellular secretions (such as proteins) are completed. The disorder of Golgi apparatus structure and function will induce many diseases. Therefore, monitoring the morphological structure of Golgi apparatus is crucial for the diagnosis and treatment of relevant diseases. In order to achieve Golgi apparatus-targeting imaging, the strategy of targeting unit inheritance was adopted and carbon dots (CDs) with Golgi apparatus-targeting ability were synthesized by one-step hydrothermal method with L-ascorbic acid with high reactivity and reducibility as the carbon source and L-cysteine as the targeting unit. CDs have a certain amount of cysteine residues on their surface, and have excitation dependence, satisfactory fluorescence and cysteine residues stability and low toxicity. As an imaging agent, CDs can be used for targeting imaging of Golgi apparatus.
Keywords carbon dot      Golgi apparatus      targeting imaging     
Corresponding Author(s): Yingying Wei,Yuduan Gao   
About author:

Changjian Wang and Zhiying Yang contributed equally to this work.
Issue Date: 01 March 2023
 Cite this article:   
Yingying Wei,Yuduan Gao,Lin Chen, et al. Carbon dots based on targeting unit inheritance strategy for Golgi apparatus-targeting imaging[J]. Front. Mater. Sci., 2023, 17(1): 230627.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-023-0627-y
https://academic.hep.com.cn/foms/EN/Y2023/V17/I1/230627
Fig.1  Synthesis of CDs and their application in Golgi apparatus-targeting imaging.
Fig.2  Content of Cys residues in CDs synthesized under different conditions.
Fig.3  TEM image and particle size statistics of CDs.
Fig.4  (a) XRD pattern and (b) Raman spectrum of CDs.
Fig.5  (a) FTIR spectra, (b) full XPS spectra, and (c)(d)(e)(f) high-resolution XPS spectra of C 1s, N 1s, O 1s, and S 2p of CDs.
Fig.6  The zeta potential of CDs.
Fig.7  (a) Normalized UV-Vis absorption, excitation, and emission spectra of CDs. (b) Emission spectra of CD aqueous solutions at different excitation wavelengths.
Fig.8  The fluorescence lifetime curve of CDs.
Fig.9  Circular dichroism spectra of (a) L-Cys, (b) L-AA, and (c) CDs.
Fig.10  Emission spectra of CDs in NaCl solutions with (a) different pH values and (b) different concentrations (insets in panels (a) and (b) show variations of the CD fluorescence intensity with the pH value of the NaCl solution and with the concentration of NaCl, respectively). (c) Variation of the CD fluorescence intensity with the illumination time.
Fig.11  Circular dichroism spectra of CDs in the solutions (a) at different pH values and (b) at different NaCl concentrations. Curves of circular dichroism intensities at 213, 250, and 280 nm with (c) the increasing temperature and (d) the decreasing temperature.
Fig.12  The cytotoxicity of CDs against HeLa cells at different concentrations.
Fig.13  Laser confocal images: (a) HeLa cells in bright field; (b) HeLa cells labeled with CDs; (c) HeLa cells labeled with NBD C6-Ceramide; (d) merged of panels (a), (b), and (c). (e) Correlation plot of the intensities of CDs and NBD C6-Ceramide in HeLa cell. (f) Co-located scatter image of CDs and NBD C6-Ceramide.
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[1] FMS-23627-OF-Wyy_suppl_1 Download
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