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Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

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Prot Cell    2013, Vol. 4 Issue (2) : 155-161    https://doi.org/10.1007/s13238-012-2126-2      PMID: 23479427
RESEARCH ARTICLE
AMPK interacts with DSCAM and plays an important role in Netrin-1 induced neurite outgrowth
Kun Zhu1, Xiaoping Chen2, Jianghong Liu1, Haihong Ye1, Li Zhu1(), Jane Y. Wu2()
1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. Department of Neurology, Center for Genetic Medicine, Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
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Abstract

Down syndrome cell adhesion molecule (DSCAM) acts as a netrin-1 receptor and mediates attractive response of axons to netrin-1 in neural development. However, the signaling mechanisms of netrin-DSCAM remain unclear. Here we report that AMP-activated protein kinase (AMPK) interacts with DSCAM through its γ subunit, but does not interact with DCC (deleted in colorectal cancer), another major receptor for netrin-1. Netrin-treatment of cultured cortical neurons leads to increased phosphorylation of AMPK. Both AMPK mutant with dominant-negative effect and AMPK inhibitor can significantly suppress netrin-1 induced neurite outgrowth. Together, these findings demonstrate that AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth. Our study uncovers a previously unknown component, AMPK, in netrin-DSCAM signaling pathway.
Keywords AMP-activated protein kinase (AMPK)      neurite outgrowth      Down syndrome cell adhesion molecule (DSCAM)      netrin     
Corresponding Author(s): Zhu Li,Email:zhuli@moon.ibp.ac.cn; Wu Jane Y.,Email:jane-wu@northwestern.edu   
Issue Date: 01 February 2013
 Cite this article:   
Kun Zhu,Xiaoping Chen,Jianghong Liu, et al. AMPK interacts with DSCAM and plays an important role in Netrin-1 induced neurite outgrowth[J]. Prot Cell, 2013, 4(2): 155-161.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2126-2
https://academic.hep.com.cn/pac/EN/Y2013/V4/I2/155
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