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Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

Postal Subscription Code 80-984

2018 Impact Factor: 7.575

Protein Cell    2021, Vol. 12 Issue (9) : 734-740    https://doi.org/10.1007/s13238-021-00832-z
LETTER
Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2
Dan Zhao1, Weifan Xu2,3, Xiaofan Zhang1, Xiaoting Wang4, Yiyue Ge5, Enming Yuan1, Yuanpeng Xiong6, Shenyang Wu7, Shuya Li1, Nian Wu1, Tingzhong Tian1, Xiaolong Feng8, Hantao Shu1, Peng Lang1, Jingxin Li5, Fengcai Zhu5,9, Xiaokun Shen10, Haitao Li3,11, Pilong Li2,3(), Jianyang Zeng1()
1. Institute for Interdisciplinary Information Sciences, Tsinghua University, Beijing 100084, China
2. Beijing Advanced Innovation Center for Structural Biology Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China
3. Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China
4. Silexon AI Technology Co., Ltd, Nanjing 210033, China
5. NHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Center for Diseases Control and Prevention, Nanjing 210009, China
6. Bioinformatics Division, BNRIST/Department of Computer Science and Technology, Tsinghua University, Beijing 100084, China
7. Protein Preparation and Identification Facility, Technology Center for Protein Science, Tsinghua University, Beijing 100084, China
8. Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China
9. Center for Global Health, Nanjing Medical University, Nanjing 210009, China
10. Convalife (Shanghai) Co., Ltd, Shanghai 201203, China
11. Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China
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Corresponding Author(s): Pilong Li,Jianyang Zeng   
Online First Date: 21 May 2021    Issue Date: 14 October 2021
 Cite this article:   
Dan Zhao,Weifan Xu,Xiaofan Zhang, et al. Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2[J]. Protein Cell, 2021, 12(9): 734-740.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-021-00832-z
https://academic.hep.com.cn/pac/EN/Y2021/V12/I9/734
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5 KR Hurst , CA Koetzner , PS Masters (2013) Characterization of a critical interaction between the coronavirus nucleocapsid protein and nonstructural protein 3 of the viral replicase-transcriptase complex. J Virol 87:9159–9172
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6 C Iserman , C Roden , M Boerneke , R Sealfon , G McLaughlin , I Jungreis, C Park , A Boppana , E Fritch , YJ Hou et al. (2020) Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate. bioRxiv.
https://doi.org/10.1101/2020.06.11.147199
7 IA Klein , A Boija , LK Afeyan , SW Hawken , M Fan , A Dall’Agnese , O Oksuz , JE Henninger , K Shrinivas , BR Sabari et al. (2020) Partitioning of cancer therapeutics in nuclear condensates. Science 368:1386–1392
https://doi.org/10.1126/science.aaz4427
8 SY Lin , CL Liu , YM Chang , J Zhao , S Perlman , MH Hou (2014) Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target. J Med Chem 57:2247–2257
https://doi.org/10.1021/jm500089r
9 YA Malik (2020) Properties of Coronavirus and SARS-CoV-2. Malays J Pathol 42:3–11
10 GW Nelson , SA, Stohlman SM Tahara (2000) High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. J General Virol 81:181–188
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11 T.M. Perdikari, , A.C. Murthy, , V.H. Ryan, , S. Watters, , M.T. Naik, , and N.L. Fawzi, (2020). SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins. bioRxiv.
https://doi.org/10.1101/2020.06.09.141101
12 A., Savastano, A.L. Opakua, , M. Rankovic, , M Zweckstetterc, . (2020). Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. bioRxiv.
https://doi.org/10.1101/2020.06.18.160648
13 SA Stohlman , RS Baric , GN Nelson , LH Soe , LM Welter , RJ Deans (1988) Specific interaction between coronavirus leader RNA and nucleocapsid protein. J Virol 62:4288–4295
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14 M Surjit , SK Lal (2008) The SARS-CoV nucleocapsid protein: a protein with multifarious activities. Infect Genet Evol 8:397–405
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