Please wait a minute...
Protein & Cell

ISSN 1674-800X

ISSN 1674-8018(Online)

CN 11-5886/Q

Postal Subscription Code 80-984

2018 Impact Factor: 7.575

Protein Cell    2024, Vol. 15 Issue (11) : 791-795    https://doi.org/10.1093/procel/pwae043
An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines
Jingjing Liu1, Tao Zheng2, Lingjie Xu3, Zhicai Chen3, Kunkun Zhang3, Xiangxi Wang2, Xiaoyu Xu3(), Yuhua Li1(), Yao Sun2(), Ling Zhu2()
. Department of Arbovirus Vaccine, National Institutes for Food and Drug Control, Beijing 102629, China
. CAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
. Nanjing Vazyme Biotechnology Co., Ltd., Nanjing 210046, China
 Download: PDF(1763 KB)  
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Corresponding Author(s): Xiaoyu Xu,Yuhua Li,Yao Sun,Ling Zhu   
Issue Date: 29 November 2024
 Cite this article:   
Ling Zhu,Yao Sun,Yuhua Li, et al. An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines[J]. Protein Cell, 2024, 15(11): 791-795.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1093/procel/pwae043
https://academic.hep.com.cn/pac/EN/Y2024/V15/I11/791
1 MG Blango, BL Bass. Identification of the long, edited dsRNAome of LPS-stimulated immune cells. Genome Res 2016;26:852–862.
https://doi.org/10.1101/gr.203992.116
2 M Bonin, J Oberstrass, N Lukacs et al. Determination of preferential binding sites for anti-dsRNA antibodies on double-stranded RNA by scanning force microscopy. RNA 2000;6:563–570.
https://doi.org/10.1017/S1355838200992318
3 SQ Deng, HJ Peng. Characteristics of and public health responses to the Coronavirus Disease 2019 outbreak in China. J Clin Med 2020;9:575.
https://doi.org/10.3390/jcm9020575
4 A Dousis, K Ravichandran, EM Hobert et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol 2023;41:560–568.
https://doi.org/10.1038/S41587-022-01525-6
5 H Kato, K Takahasi, T Fujita. RIG-I-like receptors: cytoplasmic sensors for non-self RNA. Immunol Rev 2011;243:91–98.
https://doi.org/10.1111/j.1600-065X.2011.01052.x
6 M Lybecker, B Zimmermann, I Bilusic et al. The double-stranded transcriptome of Escherichia coli. Proc Natl Acad Sci U S A 2014;111:3134–3139.
https://doi.org/10.1073/pnas.1315974111
7 X Mu, S Hur. Immunogenicity of in vitro-transcribed RNA. Acc Chem Res 2021;54:4012–4023.
https://doi.org/10.1021/acs.accounts.1C00521
8 J Pohar, N Pirher, M Bencina et al. The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists. J Biol Chem 2013;288:442–454.
https://doi.org/10.1074/jbc.M112.413922
9 J Schonborn, J Oberstrass, E Breyel et al. Monoclonal anti-bodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res 1991;19:2993–3000.
https://doi.org/10.1093/nar/19.11.2993
10 R Sumirtanurdin, MI Barliana. Coronavirus disease 2019 vaccine development: an overview. Viral Immunol 2021;34:134–144.
https://doi.org/10.1089/vim.2020.0119
11 I Tuszynska, M Magnus, K Jonak et al. NPDock: a web server for protein-nucleic acid docking. Nucleic Acids Res 2015;43:W425–W430.
https://doi.org/10.1093/nar/gkv493
12 X Xia. Detailed dissection and critical evaluation of the Pfizer/BioNTech and Moderna mRNA vaccines. Vaccines (Basel) 2021;9:734.
https://doi.org/10.3390/vaccineS9070734
13 H Zangger, C Ronet, C Desponds et al. Detection of Leishmania RNA virus in Leishmania parasites. PLoS NeglTrop Dis 2013;7:e2006.
https://doi.org/10.1371/journal.pntd.0002006
[1] PAC-0791-24137-SY_suppl_1 Download
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed