Mutagenesis and selective breeding of a high producing <italic>?</italic>-poly-<italic>L</italic>-lysine strain

doi:10.1007/s11705-012-1273-6

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 179-183 https://doi.org/10.1007/s11705-012-1273-6

RESEARCH ARTICLE

Mutagenesis and selective breeding of a high producing ?-poly-L-lysine strain

Tian WANG, Shiru JIA(

), Zhilei TAN, Yujie DAI, Shuai SONG, Guoliang WANG

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China

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Abstract

?-Poly-L-lysine (?-PL) is an L-lysine linear homopolymer, which is produced by bacteria belonging to the Streptomycetaceae family and by ergot fungi. However, the production of ?-PL by the wild bacteria strain is very low, which limits its utilization. In most bacteria including the Streptomyces genus, L-lysine is a precursor of ?-PL and is biosynthesized by the L-aspartate pathway. Aspartokinase (Ask) is the first key enzyme in this pathway and is subject to complex regulation such as the feedback inhibition by the end product amino acids. In addition, phosphoenolpyruvate carboxykinase is feedback-regulated by L-aspartate. To reduce these feedback inhibitions and to improve ?-PL productivity, resistant mutants were produced using sulfaguanidine (SG) + glycine+ L-lysine+ DL-3-hydroxynorvaline (AHV) as selective markers. Using the interaction between ?-PL and the charged dye in the solid culture medium, hundreds of colonies were simultaneously screened in a quick and effective manner. Finally, one ?-PL-producing strain, Streptomyces diastatochromogenes L9, was selected. The productivity of this strain during flask fermentation was 0.77 g/L, which was 15% higher than that of the original strain. Moreover, its fermentation performance and genetic characteristics were very stable.

Keywords ?-poly-L-lysine plasma AHV Streptomyces diastatochromogenes fermentation

Corresponding Author(s): JIA Shiru,Email:jiashiru@tust.edu.cn

Issue Date: 05 June 2012

Cite this article:

Tian WANG,Shiru JIA,Zhilei TAN, et al. Mutagenesis and selective breeding of a high producing ?-poly-L-lysine strain[J]. Front Chem Sci Eng, 2012, 6(2): 179-183.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1273-6
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/179

Fig.1 Detection of secreted -PL on the M3G agar plate embedded with methylene blue

Tab.1 Fermentation results and colonial morphology

Tab.2 The stability of high-producing mutant strain L9

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