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Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis |
Yansong HOU1,2, Wei LIANG1(), Liping ZHANG1, Shuiping CHENG1, Feng HE1, Zhenbin WU1 |
1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; 2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China |
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Abstract The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reverse-phase C18 nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide a, 19′-butanoyloxyfucoxanthin, chlorophyll c1 + c2, phephorbides a, peridinin, methyl-chlorophyllide a, fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b allomer, chlorophyll b, chlorophyll a allomer, chlorophyll a, and β,β-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.
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Keywords
high-performance liquid chromatographic (HPLC)
algae
pigment
chemotaxonomy
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Corresponding Author(s):
LIANG Wei,Email:liangwei02@tsinghua.org.cn; wuzb@ihb.ac.cn
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Issue Date: 05 March 2011
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1 |
Li H P, Gong G C, Hsiung T M. Phytoplankton pigment analysis by HPLC and its application in algal community investigations. Botanical Bulletin of Academia Sinica , 2003, 43: 283-290
|
2 |
Wright S W, Jeffrey S W. Pigment markers for phytoplankton production. The Handbook of Environmental Chemistry , 2006, 2: Part N, 71-104
|
3 |
Bidigare R R, Ondrusek M E. Spatial and temporal variability of phytoplankton pigment distributions in the central equatorial Pacific Ocean. Deep Sea Research Part II, Topical Studies in Oceanography , 1996, 43(4-6): 809-833 doi: 10.1016/0967-0645(96)00019-7
|
4 |
Llewellyn C A, Fishwick J R, Blackford J C. Phytoplankton community assemblage in the English Channel: a comparison using chlorophyll a derived from HPLC-CHEMTAX and carbon derived from microscopy cell counts. Journal of Plankton Research , 2005, 27(1): 103-119 doi: 10.1093/plankt/fbh158
|
5 |
Buchaca T, Felip M, Catalan J. A comparison of HPLC pigment analyses and biovolume estimates of phytoplankton groups in an oligotrophic lake. Journal of Plankton Research , 2005, 27(1): 91-101 doi: 10.1093/plankt/fbh154
|
6 |
Wulff A, Vilbaste S, Truu J. Depth distribution of photosynthetic pigments and diatoms in the sediments of a microtidal fjord. Hydrobiologia , 2005, 534: 117-130 doi: 10.1007/s10750-004-1417-x
|
7 |
Buchaca T, Catalan J. Factors influencing the variability of pigments in the surface sediments of mountain lakes. Freshwater Biology , 2007, 52(7): 1365-1379 doi: 10.1111/j.1365-2427.2007.01774.x
|
8 |
Jeffrey S W, Mantoura R F C, Wright S W. Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods. Paris: UNESCO Publishing, 1997, 37-84
|
9 |
Redalje D, Lohrenz S, Natter M, Tuel M, Kirkpatrick G, Millie D, Fahnenstiel G, Vandolah F. The growth dynamics of Karenia brevis within discrete blooms on the West Florida Shelf. Continental Shelf Research , 2008, 28(1): 24-44 doi: 10.1016/j.csr.2007.04.011
|
10 |
Mackey D J, Higgins H W, Mackey M D, Holdsworth D. Algal class abundances in the western equatorial Pacific: Estimation from HPLC measurements of chloroplast pigments using CHEMTAX. Deep Sea Research Part I , 1998, 45(9): 1441-1468 doi: 10.1016/S0967-0637(98)00025-9
|
11 |
Kraay G, Zapata M, Veldhuis M J W. Separation of chlorophylls c1, c2 and c3 of marine phytoplankton by reversed-phase-C18-high-performance liquid chromatography. Journal of Phycology , 1992, 28(5): 708-712 doi: 10.1111/j.0022-3646.1992.00708.x
|
12 |
Otsuki A, Watanabe M M, Sugahara K. Chlorophyll pigments in methanol extracts from ten axenic cultured diatoms and three green algae as determined by reverse phase HPLC with fluorometric detection.Journal of Phycology , 1987, 23(3): 406-414 doi: 10.1111/j.1529-8817.1987.tb02526.x
|
13 |
Mantoura R F C, Llewellyn C A. The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high performance liquid chromatography. Analytica Chimica Acta , 1983, 151: 297-314 doi: 10.1016/S0003-2670(00)80092-6
|
14 |
Stoń-Egiert J, Kosakowska A. RP-HPLC determination of phytoplankton pigments-comparison of calibration results for two columns. Marine Biology , 2005, 147(1): 251-260 doi: 10.1007/s00227-004-1551-z
|
15 |
Hooks C E, Bidigare R R, Keller M D, Guillard R R L. Coccoid eukaryotic marine ultraplankters with four different HPLC pigment signatures. Journal of Phycology , 1998, 24: 571-580
|
16 |
Claustre H, Hooker S B, Van Heukelem L, Berthon J F. Barlow R, Ras J, Sessions H, Targa C, Thomas C S, Van der Linde D, Marty J C. An intercomparison of HPLC phytoplankton pigment methods using in situ samples: application to remote sensing and database activities. Marine Chemistry , 2004, 85(1–2): 41–61 doi: 10.1016/j.marchem.2003.09.002
|
17 |
Stoń J, Kosakowska A. Phytoplankton pigments designation-an application of RP-HPLC in qualitative and quantitative analysis. Journal of Applied Phycology , 2002, 14(3): 205-210 doi: 10.1023/A:1019928411436
|
18 |
Stoń J, Kosakowska A. Phytoplankton pigments designation-an application of RP-HPLC in qualitative and quantitative analysis. Journal of Applied Phycology , 2007, 14(3): 205-210 doi: 10.1023/A:1019928411436
|
19 |
Hu H J, Li Y Y, Wei Y X. The Freshwater Algae of China. Shanghai: Shanghai Science and Technology Press, 1980 (in Chinese)
|
20 |
Hu H J.The Freshwater Algae of China – Systematics, Taxonomy and Ecology. Beijing: Science Press, 2006 (in Chinese)
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