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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

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Front. Environ. Sci. Eng.    2018, Vol. 12 Issue (6) : 3    https://doi.org/10.1007/s11783-018-1050-y
RESEARCH ARTICLE
Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge
Sijia Ai1, Hongyu Liu1(), Mengjie Wu1, Guangming Zeng1, Chunping Yang1,2()
1. College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China
2. Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China
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Abstract

Three acid-producing strains were isolated and identified.

The isolated bacteria accelerated the anaerobic digestion processes.

Bacillus coagulans improved TCOD removal, VS removal and biogas production.

The optimal inoculum concentration of Bacillus coagulans AFB-1 was 30%.

Three acid-producing strains, AFB-1, AFB-2 and AFB-3, were isolated during this study, and their roles in anaerobic digestion of waste activated sludge (WAS) were evaluated. Data of 16S rRNA method showed that AFB-1 and AFB-2 were Bacillus coagulans, and AFB-3 was Escherichia coli. The removal in terms of volatile solids (VS) and total chemical oxygen demand (TCOD) was maximized at 42.7% and 44.7% by inoculating Bacillus coagulans AFB-1. Besides, the optimal inoculum concentration of Bacillus coagulans AFB-1 was 30% (v/v). Solubilization degree experiments indicated that solubilization ratios (SR) of WAS reached 20.8%±2.2%, 17.7%±1.48%, and 11.1%±1.53%. Volatile fatty acids (VFAs) concentrations and compositions were also explored with a gas chromatograph. The results showed that VFAs improved by 98.5%, 53.0% and 11.6% than those of the control, respectively. Biochemical methane potential (BMP) experiments revealed that biogas production increased by 90.7% and 75.3% when inoculating with Bacillus coagulans AFB-1 and AFB-2. These results confirmed that the isolated acid-producing bacteria, especially Bacillus coagulans, was a good candidate for anaerobic digestion of WAS.
Keywords Anaerobic digestion      Waste activated sludge      Acid-producing bacteria      Bacillus coagulans     
Corresponding Author(s): Hongyu Liu,Chunping Yang   
Issue Date: 19 August 2018
 Cite this article:   
Sijia Ai,Hongyu Liu,Mengjie Wu, et al. Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge[J]. Front. Environ. Sci. Eng., 2018, 12(6): 3.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1050-y
https://academic.hep.com.cn/fese/EN/Y2018/V12/I6/3
Group Content
Control 300 mL seed anaerobic sludge+ 400 mL WAS after thermal hydrolysis pretreatment+ 300 mL sterile ultrapure water
AFB-1 300 mL seed anaerobic sludge+ 400 mL WAS after thermal hydrolysis pretreatment+ 300 mL bacteria suspension of Bacillus coagulans AFB-1
AFB-2 300 mL seed anaerobic sludge+ 400 mL WAS after thermal hydrolysis pretreatment+ 300 mL bacteria suspension of Bacillus coagulans AFB-2
AFB-3 300 mL seed anaerobic sludge+ 400 mL WAS after thermal hydrolysis pretreatment+ 300 mL bacteria suspension of Escherichia coli AFB-3
Tab.1  Different inoculation conditions at 50°C±0.3°C
Fig.1  Phylogenetic tree showing the relationships among 16S rRNA sequences from Bacillus coagulans AFB-1, Bacillus coagulans AFB-2 and Escherichia coli AFB-3 and the most similar sequences retrieved from databases
Fig.2  Changes in the NH4+-N content, pH value and VFAs content with anaerobic digestion: (a) NH4+-N content; (b) pH value; and (c) VFAs content. Error bars represent standard deviations of triplicate tests
Fig.3  Changes in VS removal and TCOD removal: (a) VS removal and (b) TCOD removal. The reaction temperature was 50°C±0.3°C . Error bars represent standard deviations of triplicate tests
Group SCODinitial
(g/L)		 SCODfinal
(g/L)		 TCODinitial
(g/L)		 Solubilization ratio (%)
Control 10.8±0.62 14.5±0.23 35.6±0.4 10.4±1.45
AFB-1 18.2±0.17 20.8±2.20
AFB-2 17.1±0.32 17.7±1.48
AFB-3 14.7±0.26 11.1±1.53
Tab.2  Concentration and solubilization ratio of the organic components of digested sludge by inoculating acid-producing bacteria
Fig.4  Concentration of VFAs and variations in individual VFA: (a) AFB-1; (b) AFB-2; (c) AFB-3; and (d) Control group. Error bars represent standard deviations of triplicate tests
Fig.5  Variations in cumulative biogas production. Error bars represent standard deviations of triplicate tests
Fig.6  Effect of inoculating Bacillus coagulans AFB-1 on (a) cell density, (b) TCOD removal and VS removal during anaerobically digested sludge treatment. Error bars represent standard deviations of triplicate tests
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