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Frontiers of Agricultural Science and Engineering

ISSN 2095-7505

ISSN 2095-977X(Online)

CN 10-1204/S

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Front. Agr. Sci. Eng.    2014, Vol. 1 Issue (1) : 37-45    https://doi.org/10.15302/J-FASE-2014008
RESEARCH ARTICLE
Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost
Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI(),Zhendong ZHAO()
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology & Genetic Improvement on North Yellow & Huai River Valley, Ministry of Agriculture of the People Republic of China; National Engineering Laboratory for Wheat & Maize, Jinan 250100, China
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Abstract

Spring radiation frost (SRF) is a severe environmental stress which impairs wheat yield and productivity worldwide. To better understand the mechanism of wheat (Triticum aestivum) responding to SRF, a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively. A total of 43 differentially expressed proteins (DEPs) which mainly involved in carbohydrate metabolism, amino acid metabolism, resistance proteins and antioxidant enzymes, photosynthesis and cellular respiration proteins, cell-wall related proteins, protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS. The results revealed that of the 21 DEPs in Jimai22 responding to the SRF, 13 DEPs were upregulated and 8 DEPs were downregulated, and that of the 22 DEPs in Luyuan301, 9 DEPs were upregulated and 13 DEPs were downregulated. These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301. The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.
Keywords common wheat      spring radiation frost      proteomic analysis      2-DE      MALDI-TOF-TOF MS     
Corresponding Author(s): Genying LI   
Issue Date: 22 May 2014
 Cite this article:   
Shujuan ZHANG,Guoqi SONG,Yulian LI, et al. Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost[J]. Front. Agr. Sci. Eng. , 2014, 1(1): 37-45.
 URL:  
https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2014008
https://academic.hep.com.cn/fase/EN/Y2014/V1/I1/37
Fig.1  Physiologic responses induced by cold treatment. (a) Effect of cold treatment on relative water content (RWC); (b) soluble sugar content of cold-treated and untreated plants. Values are the means ± SD of three differences from each cultivar. * significant differences from the corresponding untreated plants at P<0.05 under the cold treatment
Fig.2  Comparison of 2-DE gel maps proteins in cold-treated plants with untreated plants. Forty-three of the spots at least a 2-fold change under cold treatment with P<0.05 were analyzed. (a) 2D-images of proteins from untreated Jimai22 plants; (b) 2D-images from Jimai22 plants after cold-treatment for 48 h; (c) 2D-images of proteins from untreated Luyuan301 plants; (d) 2D-images from Luyuan301 plants after cold-treatment for 48 h. Proteins that increased under cold stress are numbered on the 2-D map
SpotProtein nameTheoretical pITheoretical Mw/kDaScoreSequence coverage /%PeptidesmatchedFoldchangeAccession No.Species
682chloroplast fructose-bisphosphate aldolase5.94422176002662.08ACM78035Triticum aestivum
220predicted protein6.45328548751-2.73BAK01718Hordeum vulgare
571victorin binding protein6.48112298141352.00AAA63798Avena sativa
692PREDICTED:quinone oxidoreductase-like protein At1g237408.2939840196822.55XP_003574429Brachypodium distachyon
127light-harvesting complex I, partial (chloroplast)8.11244392771042.61AAB29485H. vulgare
76RecName: Ribulose bisphosphate carboxylase small chain PW9, chloroplastic;8.5219784566415-3.27P26667T. aestivum
369predicted protein6.3647882145113-4.54BAJ95933H. vulgare
148predicted protein7.6729.70502337-2.42BAJ97959H. vulgare
441unnamed protein product6.32475172481254.52CAA25265H. vulgare
218hypothetical protein SORBIDRAFT_09 g0011306.9931865343113-4.34XP_002439133Sorghum bicolor
455ATP synthase beta subunit5.2952213339166-2.78ABR67209Pseudosasa japonica
432predicted protein6.005203850722.12BAJ89632H. vulgare
113predicted protein5.63259266833553.32BAK01399H. vulgare
142rbcL,ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit6.604760021593-3.94CAA44027T. aestivum
689Fructose-bisphosphate aldolase7.63419243791544.27ACG36798Zea mays
306Predicted protein5.0835439168135-2.13BAJ86516H. vulgare
316putative mitochondrial cysteine synthase precursor5.36225852852433.09ACJ54643Aegilops speltoides
366ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit9.42789599182-2.54ACI22694Secale cereale
444ribulose 1,5-bisphosphate carboxylase activase isoform 18.6247341293134-2.89AAA63163H. vulgare
97Predicted protein4.751618589715.51BAK07537H. vulgare
447ATP synthase beta subunit5.1350859234114-2.89ABH02606Festuca rubra
Tab.1  Proteins of Jimai22 responsive to cold stress identified by MALDI-TOF-MS
SpotProtein nameTheoretical pITheoretical Mw/kDaScoreSequence coverage /%PeptidesmatchedFoldchangeAccession No.Species
149Cold shock protein-15.7421884601545-2.70BAB78536Triticum aestivum
123Germin-like protein 16.0122017173152-2.27BAA74702Oryza sativa
138Pentatricopeptide repeat-containing protein8.19713866111-6.98NP_190408Arabidopsis thaliana
66Predicted:low quality protein:50S ribosomal protein L12-2,6.7722351370193-3.67XP_003566962Brachypodium distachyon
667Ribulosebisphosphate carboxylase, partial (chloroplast)6.345211144422.88AAA68039Pandanus tectorius
345Predicted: probable pyridoxal biosynthesis protein PDX1.1-like6.1133676179114-2.02XP_003558759B.distachyon
347Predicted protein6.734020210272-4.93BAK02219Hordeum vulgare
145Predicted protein4.75161856971-2.33BAK07537H. vulgare
261Predicted protein6.453285414393-3.24BAK01718H. vulgare
455ATPase subunit I (chloroplast)5.474726152622.65CAB88710Spinacia oleracea
394Ribulose-bisphosphate carboxylase activase5.0126084186183-5.30S25484Nicotiana tabacum
640Ribulose 1,5-bisphosphate carboxylase activase isoform 18.62473411301132.24AAA63163H. vulgare
84Eukaryotic translation initiation factor 5A; Short= eIF-5A.5.6017483951722.75Q9SC12Senecio vernalis
428Predicted: fructose-bisphosphate aldolase6.8637977154102-2.13XP_003563638B.distachyon
109Putative glycine-rich protein5.63195564734132.97BAD06324T. aestivum
167Predicted protein9.2527800232203-2.26BAJ85545H. vulgare
112EM_EST:AJ602339 AJ602339.15.7813731941712.97AJ602339T. aestivum
57Predicted: low quality protein: 50S ribosomal protein L12-2,6.77223512571933.85XP_003566962B.distachyon
653Predicted: ras GTPase-activating protein binding protein 1-like5.305177751832.26XP_003581124B.distachyon
49Heat shock protein 17.35.5817399230365-3.17CAA41218T. aestivum
375Malate dehydrogenase8.2336347701122.17AAD56659Glycine max
242Predicted protein6.453285413051-2.21BAK01718H. vulgare
Tab.2  Proteins of Luyuan301 responsive to low-temperature stress identified by MALDI-TOF-MS
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[1] Supplementary Material Download
[1] Yonggui XIAO,Jianjun LIU,Haosheng LI,Xinyou CAO,Xianchun XIA,Zhonghu HE. Lodging resistance and yield potential of winter wheat: effect of planting density and genotype[J]. Front. Agr. Sci. Eng. , 2015, 2(2): 168-178.
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