Polyamines and antioxidant defense system are associated with cold tolerance in centipedegrass

doi:10.15302/J-FASE-2017197

Front. Agr. Sci. Eng.

2018, Vol. 5

Issue (1) : 129-138 https://doi.org/10.15302/J-FASE-2017197

RESEARCH ARTICLE

Polyamines and antioxidant defense system are associated with cold tolerance in centipedegrass

Jingjing CHEN^1,³, Junyang FANG¹, Zhenfei GUO²(

), Shaoyun LU¹(

)

¹. College of Life Sciences, Guangdong Engineering Research Center for Grassland Science/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
². College of Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
³. Key Laboratory of Tropical Fruit Biology of Ministry of Agriculture/South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China

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Abstract

Physiological responses to low temperature were analyzed in a chilling-tolerant centipedegrass (Eremochloa ophiuroides) accession, Shao Guan (SG), in comparison to the commercial cultivar, Common. Lower levels of ion leakage and higher chlorophyll (Chl) concentration were observed in SG than in Common during chilling stress. The maximum photochemical efficiency, the actual photosystem II (PSII) efficiency, photochemical quenching efficiency, and net photosynthetic rate were decreased during chilling stress in both genotypes, with higher levels of these parameters shown by SG than Common. In addition, higher activities of superoxide dismutase (SOD), catalase (CAT), ascorbate-peroxidase (APX) and glutathione reductase (GR), and higher concentrations of ascorbic acid (AsA) and glutathione (GSH) were observed in SG than in Common. Moreover, higher concentrations of putrescine (Put), spermidine (Spd), and spermine (Spm) were observed in SG than in Common. Correlation analysis indicated that SOD, CAT, APX and GR activities, and AsA and GSH concentrations showed high correlation to Put, while APX, GR, and AsA concentrations were correlated to Spd. Exogenous Put or Spd increased antioxidant enzyme activities and chilling tolerance. The results suggested that polyamine-regulated antioxidants are important for chilling tolerance in centipedegrass and protect plants against chilling induced oxidative damage.

Keywords antioxidants centipedegrass chilling photosynthesis polyamines

Corresponding Author(s): Zhenfei GUO,Shaoyun LU

Just Accepted Date: 27 December 2017 Online First Date: 26 February 2018 Issue Date: 21 March 2018

Cite this article:

Jingjing CHEN,Junyang FANG,Zhenfei GUO, et al. Polyamines and antioxidant defense system are associated with cold tolerance in centipedegrass[J]. Front. Agr. Sci. Eng. , 2018, 5(1): 129-138.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2017197
https://academic.hep.com.cn/fase/EN/Y2018/V5/I1/129

Fig.1 Ion leakage (a) and chlorophyll (Chl) (b) in a chilling-tolerant accession (SG) and the commercial cultivar Common of centipedegrass in response to chilling at 8°C. Values are means ± SE (n=3); *, P<0.05.

Fig.2 Maximal photochemical efficiency of PSII (F_v/F_m) (a), the actual PSII efficiency (F_PSII) (b), photochemical quenching (q_p) (c), and net photosynthetic rate (A) (d) in two centipedegrass genotypes, SG and cv. Common, in response to chilling at 8°C. Values are means ± SE (n=3); *, P<0.05.

Fig.3 Activities of superoxide dismutase (SOD) (a), catalase (CAT) (b), ascorbate-peroxidase (APX) (c), glutathione reductase (GR) (d), concentrations of ascorbate (AsA) (e) and reduced glutathione (GSH) (f) in two centipedegrass genotypes, SG and cv. Common, in response to chilling at 8°C. Values are means ± SE (n=3); *, P<0.05.

Fig.4 Putrescine (Put) (a), spermidine (Spd) (b), and spermine (Spm) (c) concentrations in two centipedegrass genotypes, SG and cv. Common, in response to chilling at 8°C. Values are means ± SE (n=3); *, P<0.05.

Fig.5 Analysis of correlation of antioxidants with putrescine (Put) (a–e), spermidine (Spd) (f–h), and spermine (Spm) (i) in centipedegrass under chilling stress conditions. *, P<0.05; **, P<0.01; ***, P<0.001.

Fig.6 Effects of exogenous putrescine (Put) and spermidine (Spd) on chilling tolerance. Centipedegrass cv. Common was irrigated with 50 mL of 0.1 mmol·L⁻¹Put or Spd solution per pot, followed by chilling at 8°C. Ion leakage (a), maximal photochemical efficiency of PSII (F_v/F_m) (b), and chlorophyll (Chl) concentration (c) were measured during the chilling treatment. *, P<0.05.

Fig.7 Effects of exogenous putrescine (Put) and spermidine (Spd) on antioxidant enzyme activities in centipedegrass. Centipedegrass cv. Common was irrigated with 50 mL of 0.1 mmol·L⁻¹ Put or Spd solution per pot, followed by chilling at 8°C. Superoxide dismutase (SOD) (a), catalase (CAT) (b), and ascorbate-peroxidase (APX) (c) activities were measured during the chilling treatment. The same letter above the column indicates no significant difference at P<0.05.

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