<i>γ</i>-Valerolactone/CuCl<sub>2</sub> biphasic system for high total monosaccharides recovery from pretreatment and enzymatic hydrolysis processes of eucalyptus

doi:10.1007/s11705-024-2490-5

2024, Vol. 18

Issue (11) : 139 https://doi.org/10.1007/s11705-024-2490-5

γ-Valerolactone/CuCl₂ biphasic system for high total monosaccharides recovery from pretreatment and enzymatic hydrolysis processes of eucalyptus

Shuhua Mo, Yao Zheng, Jianyu Gong, Minsheng Lu(

)

Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industrial and Food Engineering, Guangxi University, Nanning 530004, China

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Abstract

The efficient fractionation and recovery of monosaccharides (xylose and glucose) from lignocellulosic biomass facilitates subsequent sugar-based derivative production. This study introduces a one-pot γ-valerolactone/CuCl₂ biphasic pretreatment system (100-mmol·L^–1 CuCl₂, 180 °C, 60 min) capable of achieving removal rates of 92.25% and 90.64% for xylan and lignin, respectively, while retaining 83.88% of cellulose. Compared to other metal chlorides (NaCl, LiCl, FeCl₃, and AlCl₃), the γ-valerolactone/CuCl₂ system recovered 121.2 mg·(g eucalyptus)^–1 of xylose and 55.96 mg·(g eucalyptus)^–1 of glucose during the pretreatment stage and 339.2 mg·(g eucalyptus)^–1 of glucose during the enzymatic hydrolysis stage (90.78% of glucose yield), achieving a total monosaccharide recovery of 86.31%. In addition, the recovery of γ-valerolactone was 79.33%, exhibiting minimal changes relative to the pretreatment performance. The method proposed in this study allows a high total monosaccharides recovery and a circular economy-oriented pretreatment approach, offering a viable pathway for biorefinery.

Keywords lignocellulose biorefinery total monosaccharides recovery γ-valerolactone

Corresponding Author(s): Minsheng Lu

Just Accepted Date: 11 June 2024 Issue Date: 31 July 2024

Cite this article:

Shuhua Mo,Yao Zheng,Jianyu Gong, et al. γ-Valerolactone/CuCl₂ biphasic system for high total monosaccharides recovery from pretreatment and enzymatic hydrolysis processes of eucalyptus[J]. Front. Chem. Sci. Eng., 2024, 18(11): 139.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-024-2490-5
https://academic.hep.com.cn/fcse/EN/Y2024/V18/I11/139

Fig.1 Process flow-chart of GVL/CuCl₂ system for the pretreatment of eucalyptus.

Fig.2 Content and recovery/removal of the three major components of eucalyptus at (a) different CuCl₂ concentrations (GVL/CuCl₂ = 8/2, 160 °C, 60 min), (b) different pretreatment temperatures (GVL/CuCl₂ = 8/2, 100 mmol·L^–1 CuCl₂, 60 min), and (c) different pretreatment times (GVL/CuCl₂ = 8/2, 100 mmol·L^–1 CuCl₂, 180 °C).

Tab.1 Chemical compositions of solid residues of control group

Fig.3 Schematic of the synergy between GVL and CuCl₂ to remove xylan and lignin. (a) Pretreatment condition: GVL/H₂O = 8/2, without CuCl₂, 180 °C, 60 min; (b) pretreatment condition: H₂O/CuCl₂ = 8/2, without GVL, 180 °C, 60 min.

Fig.4 (a) XRD patterns, (b) DTG (derivative thermogravimetry distributions), and (c, d) FTIR spectra of raw eucalyptus and solid residues.

Tab.2 Surface elemental distribution, surface area and porosity of raw eucalyptus and solid residues

Fig.5 Glucose yield of cellulose-rich solid residues pretreated in different conditions. (a) CuCl₂ concentrations; (b) pretreatment temperatures; (c) pretreatment times; (d) blank control, and correlation of (e) xylan and (f) lignin removals with glucose yield.

Tab.3 Recovery and reuse of GVL

Tab.4 Pretreatment effect of different GVL/Cl^– systems^a)

Fig.6 Concentration of (a) sugars and (b) inhibitors in the aqueous phase after pretreatment by different GVL/Cl^– systems (100 mmol·L^–1, 180 °C, 60 min), and (c) enzymatic glucose yields and (d) total monosaccharide recovery (xylose and glucose) during two stages (pretreatment + enzymatic hydrolysis).

Fig.7 Mass balance diagram of the proposed biorefinery strategy.

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