Fe(II) oxidation by <italic>Acidithiobacillus ferrooxidans</italic> in pure and mixed cultures in the presence of arsenate

doi:10.1007/s11707-009-0027-3

Front Earth Sci Chin

2009, Vol. 3

Issue (2) : 221-225 https://doi.org/10.1007/s11707-009-0027-3

RESEARCH ARTICLE

Fe(II) oxidation by Acidithiobacillus ferrooxidans in pure and mixed cultures in the presence of arsenate

Xiaofen YANG, Hongmei WANG(

), Linfeng GONG, Hima HASSANE, Zhengbo JIANG

Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan 430074, China

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Abstract

Fe²⁺ oxidation by Acidithiobacillus ferrooxidans in pure and mixed cultures was investigated in batch cultures in the presence of arsenate. The pH value was periodically monitored and Fe²⁺ content was analyzed by the 1,10-phenanthroline method. ICP-AES was employed for the analysis of As(V) concentration in the solution phase. Precipitates were collected and analyzed by X-ray diffraction. Slight enhancement of iron bio-oxidation was observed in mixed cultures with the two greatest As(V) concentrations (1.0 and 5.0 mg/L As), which were enriched from sediment samples in an abandoned copper mine site. As(V) concentrations decreased with time, indicating either the co-precipitation with or the adsorption by jarosite, the major sink of solid phase. Our data suggest that biogenically synthesized jarosite may play an important role in the attenuation of soluble arsenate in natural aquatic environments.

Keywords Acidithiobacillus ferrooxidans arsenate jarosite bio-oxidation geomicrobiology

Corresponding Author(s): WANG Hongmei,Email:hmwang@cug.edu.cn

Issue Date: 05 June 2009

Cite this article:

Xiaofen YANG,Hongmei WANG,Linfeng GONG, et al. Fe(II) oxidation by Acidithiobacillus ferrooxidans in pure and mixed cultures in the presence of arsenate[J]. Front Earth Sci Chin, 2009, 3(2): 221-225.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-009-0027-3
https://academic.hep.com.cn/fesci/EN/Y2009/V3/I2/221

Fig.1 Changes in pH (a, b, c) and Fe concentration (d, e, f) in the presence and absence of As(V). A.f-0. culture without As(V); A.f-0.01. culture with 0.1 mg/L As(V); A.f-0.1. culture with 0.01 mg/L As(V); W3-0. W3 culture without As(V); W3-0.01. W3 culture with 0.01 mg/L As(V); W3-0.1. W3 culture with 0.1 mg/L As(V); S3-0. S3 culture without As(V); S3-0.01. S3 culture with 0.01 mg/L As(V); S3-0.1. S3 culture with 0.1 mg/L As(V)

Fig.2 Changes in pH (a) and Fe concentration (b) in culture S3 in the presence of 0.1-5 mg/L of As(V). S3-0.1. S3 culture with 0.1 mg/L As(V); S3-0.5. S3 culture with 0.5 mg/L As(V); S3-1.0. S3 culture with 1.0 mg/L As(V); S3-5.0. S3 culture with 5 mg/L As(V)

Tab.1 Changes in the concentration of dissolved As(V)/ (mg·L)

Fig.3 X-ray patterns of the biogenic jarosites. (a), (b), (c), (d), precipitates from S3 cultures with initial 5.0, 1.0, 0.5, 0 mg/L As(V); S3-0. S3 culture without As(V). S3-0.5: S3 culture with 0.5 mg/L As(V ); S3-1.0. S3 culture with 1.0 mg/L As(V); S3-5.0. S3 culture with 5 mg/L As(V)

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