The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains

doi:10.1007/s11707-012-0345-8

Front Earth Sci

2013, Vol. 7

Issue (1) : 76-84 https://doi.org/10.1007/s11707-012-0345-8

RESEARCH ARTICLE

The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains

Junzhong ZHANG^1,3(

), Baiying MAN², Benzhong FU¹, Li LIU¹, Changzhi HAN¹

1. Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Forestry, Southwest Forestry University, Kunming 650224, China; 2. Sontan college, Guangzhou University, Guangzhou 511370, China; 3. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China

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Abstract

To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including traditional culturing, rDNA internal transcribed spacer (ITS) sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shrublands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance (S), Shannon–Wiener index (H), Simpson dominance index (D), and Pielou evenness index (J) of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66–2.71, 0.92, 0.95–0.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.

Keywords alpine shrub grasslands culturable fungi rDNA ITS sequence analysis diversity

Corresponding Author(s): ZHANG Junzhong,Email:zhangjunzhong@foxmail.com

Issue Date: 05 March 2013

Cite this article:

Junzhong ZHANG,Baiying MAN,Benzhong FU, et al. The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains[J]. Front Earth Sci, 2013, 7(1): 76-84.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-012-0345-8
https://academic.hep.com.cn/fesci/EN/Y2013/V7/I1/76

Tab.1 Basic status of the sampling sites

Tab.2 The quantity and percentages of fungi

Fig.1 Phylogenetic analysis of the alpine grasslands of soil fungi diversity based on ITS rDNA sequences

Tab.3 Phylogenetic relation to nearest neighbors of strains isolated from alpine shrubland soil

Tab.4 Soil fungi diversity of whole community and different samples

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