Algae (Microcystis and Scenedesmus) absorption spectra and its application on Chlorophyll a retrieval

doi:10.1007/s11707-013-0373-z

Front Earth Sci

2013, Vol. 7

Issue (4) : 522-530 https://doi.org/10.1007/s11707-013-0373-z

RESEARCH ARTICLE

Algae (Microcystis and Scenedesmus) absorption spectra and its application on Chlorophyll a retrieval

Di WU¹(

), Maosi CHEN², Qiao WANG¹, Wei GAO^2,3

1. Satellite Environment Center, Ministry of Environmental Protection, Beijing 100094, China; 2. USDA UV-B Monitoring and Research Program, Natural Resource Ecology Laboratory, Colorado State University, Fort Collins, CO 80521, USA; 3. Department of Ecosystem Science and Sustainability, Colorado State University, Fort Collins, CO 80521, USA

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Abstract

Blue algae and green algae are the dominant phytoplankton groups that contribute to the eutrophication and the water bloom in inland water of China. The absorption coefficients (spectra) of the algae, which do not change with its intrinsic optical characteristics and the observation geometry, are strictly additive quantities. The characteristics of the absorption spectra of the two algae are presented. The pure blue algae and the pure green algae cultured in the laboratory environment are diluted and mixed at ten volume ratios. The Quantitative Filter Technique was applied to measure their absorption spectra. The “hot-ethanol extraction” method was chosen to calculate their concentration of Chlorophyll a. The retrieval algorithm developed in this study extracts the mapping information between each individual alga and their Chlorophyll a concentration via Continuous Wavelet Transform, and retrieves the Chlorophyll a concentration of each alga in their mixture using a trust region optimizer. The results show that the retrieved and the measured Chlorophyll a concentrations of the blue algae and the green algae components in the ten mixture match well with the average relative error of 5.55%.

Keywords algae absorption coefficient spectra Chlorophyll a trust region Continuous Wavelet Transform

Corresponding Author(s): WU Di,Email:youye1984@163.com

Issue Date: 05 December 2013

Cite this article:

Di WU,Maosi CHEN,Qiao WANG, et al. Algae (Microcystis and Scenedesmus) absorption spectra and its application on Chlorophyll a retrieval[J]. Front Earth Sci, 2013, 7(4): 522-530.

URL:

https://academic.hep.com.cn/fesci/EN/10.1007/s11707-013-0373-z
https://academic.hep.com.cn/fesci/EN/Y2013/V7/I4/522

Fig.1 Absorption coefficient spectra of the pure blue algae (a) and the pure green algae (b) at ten dilution levels, respectively.

Fig.2 Absorption coefficient spectra of the mixed algae at ten mixing ratios.

Fig.3 Displays the wavelet coefficient matrix of the absorption coefficient spectrum of the undiluted pure blue algae (a) and the undiluted pure green algae (b) in the dilution experiment. The points in the areas that are not covered by the translucent mask do not influenced by the edge effect and have good fitting results (>0.9995) between the Chlorophyll a concentrations of the ten dilution pairs and the corresponding wavelet coefficients.

Fig.4 An example shows that if the absorption coefficient spectra have the property of being additive (a+b=c), the corresponding wavelet coefficients also have such property with trace residuals.

Fig.5 Take 622 nm where the Phycocyanin absorption peaks as an example, illustrates the matching and adjusting processe of the absorption coefficient spectra of the mixed algae.

Fig.6 Gives the absorption coefficient ratio spectrum between the dilution experiment and the pure ones of the mixing experiment.

Fig.7 The scatter plots of the wavelet coefficients and the concentrations of Chlorophyll a for the blue algae (a) and the green algae (b) at two sample points in the wavelet matrixes (the first point: scale= 50, wavelength= 440 nm; the second point: scale= 35, wavelength= 650 nm). The red lines are the best spline curves for those scatter points.

Tab.1 Results of the Chlorophyll a retrieval in the ten mixed algae.

Fig.8 The measured absorption coefficient spectrum of the mixed algae (the volume ratio of its blue algae and its green algae is 10:10) and the retrieved absorption coefficient spectra of the blue algae, the green algae and their mixture.

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