Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

doi:10.1007/s11783-012-0393-z

Front Envir Sci Eng

2012, Vol. 6

Issue (6) : 831-838 https://doi.org/10.1007/s11783-012-0393-z

RESEARCH ARTICLE

Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

Yuanting LI¹, Dawei LI¹, Wei SONG¹, Meng LI¹, Jie ZOU², Yitao LONG¹(

)

1. Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China; 2. Jiangsu Provincial Supervising & Testing Research Institute for Products Quality, Nanjing 210007, China

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Abstract

A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10^-8 to 2.0 × 10^-5 mol·L^-1 with a detection limit of 4.5 × 10^-8 mol·L^-1 (S/N = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.

Keywords on-site determination tyrosinase biosensor phenolic contaminants single-walled carbon nanotubes gold nanoparticles screen-printed electrodes

Corresponding Author(s): LONG Yitao,Email:ytlong@ecust.edu.cn

Issue Date: 01 December 2012

Cite this article:

Yuanting LI,Dawei LI,Wei SONG, et al. Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor[J]. Front Envir Sci Eng, 2012, 6(6): 831-838.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0393-z
https://academic.hep.com.cn/fese/EN/Y2012/V6/I6/831

Fig.1 Left diagram shows the chemical mechanism of the biosensor, and the configuration of the screen printed electrode. Right picture shows the experimental device for the on-site water monitoring. Right inset diagram is the electrochemical i-t response of the biosensor

Fig.2 SEM images of bare-SPE (A) and SWCNTs-AuNPs-Tyr-SPE (B)

Fig.3 The EIS of (a) bare-Tyr-SPE, (b) SWCNTs-Tyr-SPE, (c) AuNPs-Tyr-SPE, (d) SWCNTs-AuNPs-Tyr-SPE, (e) bare-SPE in 5.0 mmol·L KFe(CN)/KFe(CN) (1∶1) + 0.1 mol·L KCl solution. The frequency range was from 0.10 to 100 kHz. In all cases the measured data points are shown as symbols with the calculated fit to the equivalent circuit as solid lines. Inset: equivalent circuit used to model impedance data in the presence of redox couples. R, solution resistance; R, charge-transfer resistance; CPE, constant phase element; Z, Warburg impedance

Fig.4 (A) Cyclic voltammograms of SWCNTs-AuNPs-Tyr-SPE at a scan rate of 50 mV·s in 0.1 mol·L PBS, pH 7.0 without catechol (a) and with 1.0 × 10 mol·L catechol (b); (B) cyclic voltammograms of different disposable sensors at 50 mV·s: (a) bare-SPE; (b) SWCNTs-Tyr-SPE; (c) AuNPs-Tyr-SPE and (d) SWCNTs-AuNPs-Tyr-SPE in 0.1 mol·L PBS, pH 7.0 containing 1.0 × 10 mol·L catechol

Fig.5 (A) Influence of working potential on amperometric response of the biosensor in the absence (curve a) and presence (curve b) of 2.0 μmol·L catechol in 0.1 mol·LPBS, pH 7.0; (B) influence of pH on the amperometric response of 2.0 μmol·L catechol in 0.1 mol·L PBS. Working potential: -0.10 V . Ag/AgCl; (C) influence of concentration of Tyr on the amperometric response of 2.0 μmol·L catechol in 0.10 M PBS, pH 7.0. Working potential: -0.10 V . Ag/AgCl

Tab.1 The response characteristics of the disposable biosensor to phenolic contaminants

Fig.6 (A) Typical current-time response curve for the successive addition of 1.0 mmol·L catechol in 0.1 mol·L PBS, pH 7.0 at SWCNTs-AuNPs-Tyr-SPE at an applied potential of -0.10 V. Ag/AgCl. Inset: the magnification of the first six steps; (B) the calibration curve for catechol obtained at SWCNTs-AuNPs-Tyr-SPE.

Tab.2 Recovery of the disposable Tyr biosensor

1	Sun M, Yao R S, You Y H, Deng S S, Gao W X. Degradation of 4-aminophenol by hydrogen peroxide oxidation using enzyme from Serratia marcescens as catalyst. Frontiers of Environmental Science & Engineering in China , 2007, 1(1): 95-98 doi: 10.1007/s11783-007-0018-0
2	Armentrout D N, McLean J D, Long M W. Trace determination of phenolic compounds in water by reversed phase liquid chromatography with electrochemical detection using a carbon-polyethylene tubular anode. Analytical Chemistry , 1979, 51(7): 1039-1045 doi: 10.1021/ac50043a060
3	Amlathe M S, Upadhyay M S, Gupta V K. Spectrophotometric determination of trace amounts of phenol in waste water and biological fluids. Analyst (London) , 1987, 112(10): 1463-1465 doi: 10.1039/an9871201463
4	Ameer Q, Adeloju S B. Development of a potentiometric catechol biosensor by entrapment of tyrosinase within polypyrrole film. Sensors and Actuators B, Chemical , 2009, 140(1): 5-11 doi: 10.1016/j.snb.2009.03.056
5	Yin H S, Zhou Y L, Xu J, Ai S, Cui L, Zhu L. Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A. Analytica Chimica Acta , 2010, 659(1-2): 144-150 doi: 10.1016/j.aca.2009.11.051
6	Deng C, Chen J, Nie Z, Si S. A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode. Biosensors & Bioelectronics , 2010, 26(1): 213-219 doi: 10.1016/j.bios.2010.06.013
7	Kochana J, Gala A, Adamski J. Titania sol-gel–derived tyrosinase-based amperometric biosensor for determination of phenolic compounds in water samples. Examination of interference effects. Analytical and Bioanalytical Chemistry , 2008, 391(4): 1275-1281 doi: 10.1007/s00216-007-1798-6
8	Tembe S, Inamdar S, Haram S, Karve M, D’Souza S F. Electrochemical biosensor for catechol using agarose–guar gum entrapped tyrosinase. Journal of Biotechnology , 2007, 128(1): 80-85 doi: 10.1016/j.jbiotec.2006.09.020
9	Liu Z M, Liu Y L, Yang H F, Yang Y, Shen G, Yu R. A phenol biosensor based on immobilizing tyrosinase to modified core-shell magnetic nanoparticles supported at a carbon paste electrode. Analytica Chimica Acta , 2005, 533(1): 3-9 doi: 10.1016/j.aca.2004.10.077
10	Lu L M, Zhang L, Zhang X B, Huan S, Shen G, Yu R. A novel tyrosinase biosensor based on hydroxyapatite–chitosan nanocomposite for the detection of phenolic compounds. Analytica Chimica Acta , 2010, 665(2): 146-151 doi: 10.1016/j.aca.2010.03.033
11	Li Y F, Liu Z M, Liu Y L, Yang Y, Shen G, Yu R. A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles. Analytical Biochemistry , 2006, 349(1): 33-40 doi: 10.1016/j.ab.2005.11.017
12	Wang S F, Tan Y M, Zhao D M, Liu G D. Amperometric tyrosinase biosensor based on Fe₃O₄ nanoparticles–chitosan nanocomposite. Biosensors & Bioelectronics , 2008, 23(12): 1781-1787 doi: 10.1016/j.bios.2008.02.014
13	Iijima S. Carbon nanotubes: past, present, and future. Physica B, Condensed Matter , 2002, 323(1-4): 1-5 doi: 10.1016/S0921-4526(02)00869-4
14	Wang D, Li Z C, Chen L W. Templated synthesis of single-walled carbon nanotube and metal nanoparticle assemblies in solution. Journal of the American Chemical Society , 2006, 128(47): 15078-15079 doi: 10.1021/ja066617j
15	Bagal-Kestwala D, Kestwal R M, Hsieh B C, Chen R L C, Cheng T J, Chiang B H. Electrochemical β(1→3)-D-glucan biosensors fabricated by immobilization of enzymes with gold nanoparticles on platinum electrode. Biosensors & Bioelectronics , 2010, 26(1): 118-125 doi: 10.1016/j.bios.2010.05.022
16	Choudhry N A, Kampouris D K, Kadara R O, Jenkinson N, Banks C E. Next generation screen printed electrochemical platforms: non-enzymatic sensing of carbohydrates using copper(II) oxide screen printed electrodes. Analytical Methods , 2009, 1(3): 183-187 doi: 10.1039/b9ay00095j
17	Avramescu A, Andreescu S, Noguer T, Bala C, Andreescu D, Marty J L. Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations. Analytical and Bioanalytical Chemistry , 2002, 374(1): 25-32 doi: 10.1007/s00216-002-1312-0
18	Alkasir R S J, Ganesana M, Won Y H, Stanciu L, Andreescu S. Enzyme functionalized nanoparticles for electrochemical biosensors: a comparative study with applications for the detection of bisphenol A. Biosensors & Bioelectronics , 2010, 26(1): 43-49 doi: 10.1016/j.bios.2010.05.001
19	Li D W, Li Y T, Song W, Long Y T. Simultaneous determination of dihydroxybenzene isomers using disposable screen-printed electrode modified by multiwalled carbon nanotubes and gold nanoparticles. Analytical Methods , 2010, 2(7): 837-843 doi: 10.1039/c0ay00076k
20	Lee P C, Meisel D. Adsorption and surface-enhanced raman of dyes on silver and gold sols. Journal of Physical Chemistry , 1982, 86(17): 3391-3395 doi: 10.1021/j100214a025
21	Dijksma M, Boukamp B A, Kamp B, van Bennekom W P. Effect of hexacyanoferrate(II/III) on self-assembled monolayers of thioctic acid and 11-mercaptoundecanoic acid on gold. Langmuir , 2002, 18(8): 3105-3112 doi: 10.1021/la010898l
22	Dempsey E, Diamond D, Collier A. Development of a biosensor for endocrine disrupting compounds based on tyrosinase entrapped within a poly(thionine) film. Biosensors & Bioelectronics , 2004, 20(2): 367-377 doi: 10.1016/j.bios.2004.02.007
23	Du D, Wang M H, Cai J, Qin Y, Zhang A. One-step synthesis of multiwalled carbon nanotubes-gold nanocomposites for fabricating amperometric acetylcholinesterase biosensor. Sensors and Actuators B, Chemical , 2010, 143(2): 524-529 doi: 10.1016/j.snb.2009.09.051
24	Shu F R, Wilson G S. Rotating ring-disk enzyme electrode for surface catalysis studies. Analytical Chemistry , 1976, 48(12): 1679-1686 doi: 10.1021/ac50006a014

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