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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

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Front Envir Sci Eng    2013, Vol. 7 Issue (1) : 143-150    https://doi.org/10.1007/s11783-012-0456-1
RESEARCH ARTICLE
Fermentative hydrogen production from beet sugar factory wastewater treatment in a continuous stirred tank reactor using anaerobic mixed consortia
Gefu ZHU1(), Chaoxiang LIU1, Jianzheng LI2, Nanqi REN2, Lin LIU1, Xu HUANG1
1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; 2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
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Abstract

A low pH, ethanol-type fermentation process was evaluated for wastewater treatment and bio-hydrogen production from acidic beet sugar factory wastewater in a continuous stirred tank reactor (CSTR) with an effective volume of 9.6 L by anaerobic mixed cultures in this present study. After inoculating with aerobic activated sludge and operating at organic loading rate (OLR) of 12 kgCOD?m-3·d-1, HRT of 8h, and temperature of 35°C for 28 days, the CSTR achieved stable ethanol-type fermentation. When OLR was further increased to 18 kgCOD?m-3·d-1 on the 53rd day, ethanol-type fermentation dominant microflora was enhanced. The liquid fermentation products, including volatile fatty acids (VFAs) and ethanol, stabilized at 1493 mg·L-1 in the bioreactor. Effluent pH, oxidation-reduction potential (ORP), and alkalinity ranged at 4.1–4.5, -250–(-290) mV, and 230–260 mgCaCO3?L-1. The specific hydrogen production rate of anaerobic activated sludge was 0.1 L?gMLVSS-1·d-1 and the COD removal efficiency was 45%. The experimental results showed that the CSTR system had good operation stability and microbial activity, which led to high substrate conversion rate and hydrogen production ability.
Keywords fermentative hydrogen production      continuous stirred tank reactor (CSTR)      specific hydrogen production rate      beet sugar factory wastewater      ethanol-type fermentation     
Corresponding Author(s): ZHU Gefu,Email:gfzhu@iue.ac.cn   
Issue Date: 01 February 2013
 Cite this article:   
Gefu ZHU,Chaoxiang LIU,Jianzheng LI, et al. Fermentative hydrogen production from beet sugar factory wastewater treatment in a continuous stirred tank reactor using anaerobic mixed consortia[J]. Front Envir Sci Eng, 2013, 7(1): 143-150.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0456-1
https://academic.hep.com.cn/fese/EN/Y2013/V7/I1/143
Fig.1  Schematic diagram of the continuous stirred-tank reactor system
Fig.1  Schematic diagram of the continuous stirred-tank reactor system
componentpercentage/%
dried materials78-85
total sugar48-58
TOC28-34
TKN0.2-2.8
P2O50.02-0.07
CaO0.15-0.8
MgO0.01-0.1
K2O2.2-4.5
SiO20.1-0.5
Al2O30.05-0.06
Fe2O30.001-0.02
ash content4-8
Tab.1  Composition of the normal molasses
Fig.2  COD and COD removal rate in the CSTR system
Fig.2  COD and COD removal rate in the CSTR system
Fig.3  Biogas and hydrogen yields in the CSTR system
Fig.3  Biogas and hydrogen yields in the CSTR system
Fig.4  the variation of soluble metabolites concentrations in the CSTR system
Fig.4  the variation of soluble metabolites concentrations in the CSTR system
Fig.5  the variation of pH, ALK and ORP in the CSTR system
Fig.5  the variation of pH, ALK and ORP in the CSTR system
Fig.6  Scanning electron microscopy of the suspended bacteria in the CSTR reactor (Magnification: × 3000)
Fig.6  Scanning electron microscopy of the suspended bacteria in the CSTR reactor (Magnification: × 3000)
Fig.7  the variation of biomass concentration (MLVSS) in the CSTR system
Fig.7  the variation of biomass concentration (MLVSS) in the CSTR system
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