Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N<sub>2</sub>O production

doi:10.1007/s11783-013-0505-4

Front Envir Sci Eng

2013, Vol. 7

Issue (4) : 616-624 https://doi.org/10.1007/s11783-013-0505-4

RESEARCH ARTICLE

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N₂O production

Gang GUO¹, Yayi WANG¹(

), Chong WANG¹, Hong WANG¹, Mianli PAN², Shaowei CHEN¹

1. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; 2. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China

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Abstract

The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifying phosphorus (P) removal performance and N₂O production was examined using a denitrifying enhanced biologic phosphorus removal (EBPR) sludge acclimatized with mixed acetate (HAc) and propionate (Pro) (in the molar ratio 3∶1) as carbon sources. The results showed that when the AnRT was prolonged from 90 to 150 min, the anaerobic polyhydroxyalkanoate (PHA) synthesis was decreased by 15.3%. Moreover, the ineffective PHA consumption occurred in anaerobic phases and contributed to an increased NO2--N accumulation and higher free nitrous acid (FNA) concentrations (≥0.001–0.0011 mg HNO₂-N/L) in the subsequent anoxic phases, causing a severe inhibition on anoxic P-uptake and denitrification. Accordingly, the total nitrogen (TN) and total phosphorus (TP) removal efficiencies dropped by approximately 6.3% and 85.5%, respectively; and the ratio of anoxic N₂O-N production to TN removal increased by approximately 3.8%. The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated by Accumulibacter (62.0% (SE_mean = 1.5%)). In conclusion, the short-term excessive anaerobic reaction time negatively impacted denitrifying P removal performance and stimulated more N₂O production, and its effect on P removal was more obvious than that on nitrogen removal.

Keywords Denitrifying phosphorus removal anaerobic reaction time nitrous oxide polyhydroxyalkanoate free nitrous acid fluorescence in-situ hybridization

Corresponding Author(s): WANG Yayi,Email:yayi.wang@tongji.edu.cn

Issue Date: 01 August 2013

Cite this article:

Gang GUO,Yayi WANG,Chong WANG, et al. Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N₂O production[J]. Front Envir Sci Eng, 2013, 7(4): 616-624.

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https://academic.hep.com.cn/fese/EN/10.1007/s11783-013-0505-4
https://academic.hep.com.cn/fese/EN/Y2013/V7/I4/616

Fig.1 Schematic diagram of the batch experimental system and the control equipment

Fig.2 Variations in the N, P, NO, VFAs, glycogen and PHA in one cycle in the parent SBR

Tab.1 The N and P removal and NO production in one cycle in the parent SBR

Tab.2 Comparison of the anaerobic carbon transformations, P release, biomass composition with literature studies and metabolic model predictions, with acetate and propionate as carbon sources

Fig.3 FISH images from the parent SBR system. (a) shows Red– (targeted by Cy3-PAOmix probes); (b) Green– (targeted by Cy5-GAOmix probes); (C) Yellow– (Cy5-DFmix probes). All bacteria are shown in blue–(targeted by FITC-EUBmix probes) (Bar= 10 μm).

Fig.4 Variations in the N, P, NO, VFAs, glycogen and PHA during one cycle in batch reactors (a, d, g: Run 1; b, e, h: Run 2; c, f, i: Run 3)

Tab.3 Comparison of the N and P removals and NO production in batch experiments

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