Functional magnetic nanoparticles for facile viable but nonculturable bacteria separation and purification

doi:10.1007/s11783-016-0874-6

Front. Environ. Sci. Eng.

2016, Vol. 10

Issue (6) : 8 https://doi.org/10.1007/s11783-016-0874-6

RESEARCH ARTICLE

Functional magnetic nanoparticles for facile viable but nonculturable bacteria separation and purification

Chunming Wang,Huirong Lin(

),Chengsong Ye(

)

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

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Abstract

The functional surface-modified MNPs were capable of capture E. coli with high efficiency.

After induced to VBNC state by chlorination, E. coli cells could be separated by MNPs with an additional incubation process.

This study provides a facile and economic method for VBNC cell enrichment and purification.

GRAPHIC ABSTRACT

Viable But Nonculturable (VBNC) Bacteria, which represent a unique population of microorganisms in drinking water systems, have become a potential threat to human health. Current studies on VBNC cells usually fail to obtain pure VBNC state bacteria, which may lead to inaccurate results. We therefore introduce a novel method of VBNC cell separation and purification in this paper. PAH-coated magnetic nanoparticles (MNPs) were synthesized and found to be capable of capturing and releasing bacterial cells with high efficiency. With the aid of an additional incubation step, VBNC cells were easily isolated and purified from normal bacteria using functional MNPs. Our method represents a new technique that can be utilized in studies of VBNCs.

Keywords Drinking water biosafety VBNC, Nanoparticles Magnetic separation and purification

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Corresponding Author(s): Huirong Lin,Chengsong Ye

Issue Date: 27 September 2016

Cite this article:

Chunming Wang,Huirong Lin,Chengsong Ye. Functional magnetic nanoparticles for facile viable but nonculturable bacteria separation and purification[J]. Front. Environ. Sci. Eng., 2016, 10(6): 8.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-016-0874-6
https://academic.hep.com.cn/fese/EN/Y2016/V10/I6/8

Fig.1 SEM characterization of MNPs morphology

Fig.2 (a) Images of bacteria captured by MNPs, (b) comparison of capture dfficienies at different bacterial concentration

Fig.3 Desorption kinetics of culturable bacteria after incubation. Error bars represent standard errors of three biological replicates (P<0.05). (a) Bacteria numbers change with time, (b) Percentage of magnetic bacteria decrease with time

Fig.4 Characterization of the captured VBNC bacteria by fluorescence microscopy

Fig.5 SEM characterization of culturable and VBNC E. coli（a, b: normal cells, c, d: VBNC cells）

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