Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm batch reactor-constructed wetland system

doi:10.1007/s11783-019-1097-4

Front. Environ. Sci. Eng.

2019, Vol. 13

Issue (1) : 14 https://doi.org/10.1007/s11783-019-1097-4

RESEARCH ARTICLE

Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm batch reactor-constructed wetland system

Xiaohui Wang¹, Shuai Du¹, Tao Ya¹, Zhiqiang Shen², Jing Dong³, Xiaobiao Zhu¹(

)

¹. 1Department of Environmental Science and Engineering, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China
². 2State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
³. 3Beijing Municipal Research Institute of Environmental Protection, Beijing 100037, China

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Abstract

SBBR-CW system was proposed to effectively treat wastewater containing TCBPA.

CW unit contributed more than SBBR to the removal of TCBPA.

TCBPA changed the composition and structure of bacterial community in the system.

GAOs massively grew in SBBR, but did not deteriorate TP removal efficiency.

Tetrachlorobisphenol A (TCBPA) released into the sewage may cause environmental pollution and health risk to human beings. The objective of this study was to investigate the removal of TCBPA and bacterial community structures in a laboratory-scale hybrid sequencing biofilm batch reactor (SBBR)-constructed wetland (CW) system. The results showed that the removal efficiency of chemical oxidation demand (COD), ammonia, total nitrogen and total phosphorus in the SBBR-CW system was 96.7%, 97.3%, 94.4%, and 88.6%, respectively. At the stable operation stage, the system obtained a 71.7%±1.8% of TCBPA removal efficiency with the influent concentration at 200 mg/L. Illumina MiSeq sequencing of 16S rRNA gene revealed that the presence of TCBPA not only reduced the bacterial diversity in the SBBR-CW system, but also altered the composition and structure of bacterial community. After the addition of TCBPA, Proteobacteria increased from 31.3% to 38.7%, while Acidobacteria and Parcubacteria decreased greatly in the SBBR. In contrast, Acidobacteria replaced Proteobacteria as the dominant phylum in the upper soils of CW. The results indicated that TCBPA stimulated the growth of GAOs in the SBBR without deteriorating the phosphorus removal due to the presence of sufficient carbon sources. The ammonia oxidizing bacteria, Nitrosomonas, and denitrification bacteria, Hyphomicrobium and Pseudomonas, were inhibited by TCBPA, resulting in a decreasing the removal efficiency of TN and ammonia.

Keywords SBBR Constructed wetland Tetrachlorobisphenol A Microbial community structure

Corresponding Author(s): Xiaobiao Zhu

Issue Date: 18 December 2018

Cite this article:

Xiaohui Wang,Shuai Du,Tao Ya, et al. Removal of tetrachlorobisphenol A and the effects on bacterial communities in a hybrid sequencing biofilm batch reactor-constructed wetland system[J]. Front. Environ. Sci. Eng., 2019, 13(1): 14.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-019-1097-4
https://academic.hep.com.cn/fese/EN/Y2019/V13/I1/14

Fig.1 Diagrammatic sketch of the hybrid SBBR-CW process. 1-Sludge discharger; 2-Pump; 3-Air blower; 4-Carbon fiber; 5-Heater rod; 6-DO detector; 7-Pump; 8- Ceramsite; 9-Gravel; 10-Zeolite; 11-Natural soil; 12-Plants.

Fig.2 The removal of (a) COD, (b) TN, (c) NH₃-N and (d) TP in the SBBR-CW hybrid system. The dash line indicated that TCBPA was added on the 20th day.

Fig.3 The removal of TCBPA in the hybrid SBBR-CW system.

Tab.1 Changes of the alpha-diversity indices of the bacterial community. The OTUs were defined based on a sequence similarity of 97%

Fig.4 The variation of (a) bacterial phyla and (b) classes in Proteobacteria before and after the addition of TCBPA.

Fig.5 The heatmap of major bacterial genera in the hybrid SBBR-CW system.

Tab.2 Relative distribution of species contributing to nitrification and denitrification in 6 samples

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