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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

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Front. Environ. Sci. Eng.    2019, Vol. 13 Issue (4) : 52    https://doi.org/10.1007/s11783-019-1133-4
RESEARCH ARTICLE
Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater
Zhenfeng Han1,2, Ying Miao3, Jing Dong4, Zhiqiang Shen1(), Yuexi Zhou1(), Shan Liu3, Chunping Yang2
1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
2. College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China
3. College of Environmental Science and Engineering, Chang’an University, Xi’an 710061, China
4. Beijing Municipal Research Institute of Environmental Protection, Beijing 100037, China
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Abstract

Anaerobically digested swine wastewater was treated by a novel constructed wetland.

Tidal operation was better for total nitrogen removal than intermittent flow.

Mechanism of nitrogen removal by biozeolite-based constructed wetland was discussed.

Simultaneous nitrification and denitrification were determined in zeolite layer.

Nitrogen removal of wastewater containing high-strength ammonium by the constructed wetlands (CWs) has been paid much attention. In this study, the ability of a partially saturated CW to treat anaerobically-digested decentralized swine wastewater under varying operating parameters from summer to winter was investigated. The partially saturated CW achieved better NH4+-N and TN removal by tidal flow than intermittent flow. With surface loading rates of 0.108, 0.027, and 0.029 kg/(m2·d) for COD, NH4+-N, and TN, the partially saturated CW by tidal operation achieved corresponding removal efficiencies of 85.94%, 61.20%, and 57.41%, respectively, even at 10°C. When the rapid-adsorption of NH4+-N and the bioregeneration of zeolites reached dynamically stable, the simultaneous nitrification and denitrification in the aerobic zeolite layer was observed and accounted for 58.82% of the total denitrification of CW. The results of Illumina high-throughput sequencing also indicated that nitrifiers (Nitrospira and Rhizomicrobium) and denitrifiers (Rhodanobacter and Thauera) simultaneously existed in the zeolite layer. The dominant existence of versatile organic degraders and nitrifiers/denitrifiers in the zeolite layer was related to the removal of most COD and nitrogen in this zone. The contribution of the possible nitrogen removal pathways in the CW was as follows: nitrification-denitrification (86.55%)>substrate adsorption (11.70%)>plant uptake (1.15%)>microbial assimilation (0.60%).
Keywords Constructed wetland      Decentralized swine wastewater      Biozeolite      Simultaneous nitrification and denitrification      Nitrogen removal pathway     
Corresponding Author(s): Zhiqiang Shen,Yuexi Zhou   
Issue Date: 06 May 2019
 Cite this article:   
Zhenfeng Han,Ying Miao,Jing Dong, et al. Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater[J]. Front. Environ. Sci. Eng., 2019, 13(4): 52.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-019-1133-4
https://academic.hep.com.cn/fese/EN/Y2019/V13/I4/52
Fig.1  Schematic configuration of partially saturated CW (all the values in mm).
Fig.2  Performances of the CW (a) COD, (b) ammonium, (c) oxidized nitrogen in effluent, (d) TN.
Fig.3  ORPs at different depths in the zeolite layer
Nitrogen species Amount (g)
NH4+-N removed in the CW 137.25
Effluent NOx?-N from the ZL 66.65
Nitrogen denitrified in ZL 70.60
Nitrogen denitrified in the whole CW 120.02
Tab.1  The amount of nitrogen in the CW in phase 2
Nitrogen removal pathways The amount of TN removed (g) Fraction (%)
Substrate adsorption 239.51 11.70
Plant uptake 23.54 1.15
Microbial assimilation 12.25 0.60
Nitrification-denitrification 1771.17 86.55
Tab.2  Fractions of possible nitrogen removal pathways
Fig.4  Microbial composition at the phylum level.
Fig.5  Microbial composition at the genus level.
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