|
|
In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes |
Kaifeng RAO1, Na LI2(), Mei MA1, Zijian WANG1() |
1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China |
|
|
Abstract Effluents from wastewater treatment plant (WWTP) have been reported to have a broad spectrum of endocrine disrupting compounds (EDCs). The majority of studies have focused on the occurrence of estrogenic activity, while ignoring nuclear hormone receptors (NRs) pathways. In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic and agonistic effects on estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), estrogen-related receptor (ERR) and aryl hydrocarbon receptor (AHR), were conducted to evaluate the removal efficiencies of EDCs by different treatment processes of a WWTP located in Beijing. Estrogenic, anti-estrogenic, anti- androgenic, anti-progesteronic, anti-ERR and the activation of AHR activities were detected in samples from all treatment processes and the receiving water. The concentration of estrogenic contaminants with estradiol (E2) equivalent concentrations ranged from 0.82 × 10-9 to 3.54 × 10-9 g E2-EQ·L-1. The concentration of anti-estrogenic contaminants with 4-hydroxytamoxifen (4-OHT) equivalent concentrations ranged from 1.24 × 10-6 to 2.36 × 10-6 g 4-OHT-EQ·L-1. The concentration of anti-androgenic contaminants ranged from 2.21 × 10-8 to 3.52 × 10-6 g flutamide-EQ·L-1. The concentration of anti-progesteronic contaminants ranged from 3.15 × 10-5 to 2.71 × 10-4 g RU486-EQ·L-1. The concentration of anti-ERR contaminants ranged from 7.09 × 10-5 to 6.50 × 10-4 g 4-OHT-EQ·L-1. The concentration of AHR activators ranged from 1.7 × 10-10 to 3.4 × 10-10 g TCDD-EQ·L-1. These processes including secondary clarifier, coagulation, as well as coal and sand filtration could eliminated 67.2% of estrogenic contaminants, 47.0% of anti-estrogenic contaminants, 98.3% of anti-androgenic contaminants, 88.4% of anti-progesteronic contaminants, 65.4% of anti-ERR contaminants and 46.9% of AHR activators. WWTP effluents contain multiple receptor disruptors may have very complex adverse effects on exposed organisms.
|
Keywords
waste water
in vitro
recombinant yeast assay
ethoxyresorufin-O-deethylase (EROD)
receptor
endocrine
|
Corresponding Author(s):
LI Na,Email:lina@ihep.ac.cn; WANG Zijian,Email:wangzj@rcees.ac.cn
|
Issue Date: 01 February 2014
|
|
1 |
Colborn T, vom Saal F S, Soto A M. Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environmental Health Perspectives , 1993, 101(5): 378-384 doi: 10.1289/ehp.93101378 pmid:8080506
|
2 |
Heberer T, Feldmann D, Redderoen K, Altmann H J. Zimmermann. Production of Drinking Water from Highly Contaminated Surface Waters: Removal of Organic, Inorganic, and Microbial Contaminants Applying Mobile Membrane Filtration Units. Weinheim: Wiley-VCH, 2002
|
3 |
Stackelberg P E, Furlong E T, Meyer M T, Zaugg S D, Henderson A K, Reissman D B. Persistence of pharmaceutical compounds and other organic wastewater contaminants in a conventional drinking-water-treatment plant. The Science of The Total Environment , 2004, 329(1-3): 99-113 doi: 10.1016/j.scitotenv.2004.03.015 pmid:15262161
|
4 |
Johnson A C, Williams R J, Simpson P, Kanda R. What difference might sewage treatment performance make to endocrine disruption in rivers? Environmental Pollution , 2007, 147(1): 194-202 doi: 10.1016/j.envpol.2006.08.032 pmid:17030080
|
5 |
Clara M, Strenn B, Gans O, Martinez E, Kreuzinger N, Kroiss H. Removal of selected pharmaceuticals, fragrances and endocrine disrupting compounds in a membrane bioreactor and conventional wastewater treatment plants. Water Research , 2005, 39(19): 4797-4807 doi: 10.1016/j.watres.2005.09.015 pmid:16242170
|
6 |
Jobling S, Burn R W, Thorpe K, Williams R, Tyler C. Statistical modeling suggests that antiandrogens in effluents from wastewater treatment works contribute to widespread sexual disruption in fish living in English rivers. Environmental Health Perspectives , 2009, 117(5): 797-802 pmid:19479024
|
7 |
Kristensen T, Baatrup E, Bayley M. p,p′-DDE fails to reduce the competitive reproductive fitness in Nigerian male guppies. Ecotoxicology and Environmental Safety , 2006, 63(1): 148-157 doi: 10.1016/j.ecoenv.2005.03.010 pmid:16399166
|
8 |
Li J, Li N, Ma M, Giesy J P, Wang Z. In vitro profiling of the endocrine disrupting potency of organochlorine pesticides. Toxicology Letters , 2008, 183(1-3): 65-71 pmid:18992306
|
9 |
Li J, Ma M, Wang Z. In vitro profiling of endocrine disrupting effects of phenols. Toxicology In vitro: an international journal published in association with BIBRA , 2010, 24(1): 201-207 pmid:10.1016/j.tiv.2009.09.00819765641
|
10 |
Benfenati E, Barcelò D, Johnson I, Galassi S, Levsen K. Emerging organic contaminants in leachates from industrial waste landfills and industrial effluent. Trends in Analytical Chemistry , 2003, 22(10): 757-765 doi: 10.1016/S0165-9936(03)01004-5
|
11 |
Sánchez-Avila J, Bonet J, Velasco G, Lacorte S. Determination and occurrence of phthalates, alkylphenols, bisphenol A, PBDEs, PCBs and PAHs in an industrial sewage grid discharging to a Municipal Wastewater Treatment Plant. The Science of The Total Environment, 2009, 407(13): 4157-4167 doi: 10.1016/j.scitotenv.2009.03.016 pmid:19362327
|
12 |
Overington J P, Al-Lazikani B, Hopkins A L. How many drug targets are there? Nature Reviews. Drug Discovery , 2006, 5(12): 993-996 doi: 10.1038/nrd2199 pmid:17139284
|
13 |
Kolodziej E P, Sedlak D L. Rangeland grazing as a source of steroid hormones to surface waters. Environmental Science & Technology , 2007, 41(10): 3514-3520 doi: 10.1021/es063050y pmid:17547172
|
14 |
Durhan E J, Lambright C S, Makynen E A, Lazorchak J, Hartig P C, Wilson V S, Gray L E, Ankley G T. Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlot. Environmental Health Perspectives , 2005, 114(S-1): 65-68 doi: 10.1289/ehp.8055 pmid:16818248
|
15 |
Westerhoff P, Yoon Y, Snyder S, Wert E. Fate of endocrine-disruptor, pharmaceutical, and personal care product chemicals during simulated drinking water treatment processes. Environmental Science & Technology , 2005, 39(17): 6649-6663 doi: 10.1021/es0484799 pmid:16190224
|
16 |
Molinamolina J M, Hillenweck A, Jouanin I, Zalko D, Cravedi J P, Fernández M F, Pillon A, Nicolas J C, Olea N, Balaguer P. Steroid receptor profiling of vinclozolin and its primary metabolites. Toxicology and Applied Pharmacology , 2006, 216(1): 44-54 doi: 10.1016/j.taap.2006.04.005 pmid:16750840
|
17 |
Ma M, Li J, Wang Z. Assessing the detoxication efficiencies of wastewater treatment processes using a battery of bioassays/biomarkers. Archives of Environmental Contamination and Toxicology , 2005, 49(4): 480-487 doi: 10.1007/s00244-004-0204-z pmid:16205990
|
18 |
Tillitt D E, Ankley G T, Verbrugge D A, Giesy J P, Ludwig J P, Kubiak T J. H4IIE rat hepatoma cell bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in colonial fish-eating waterbird eggs from the Great Lakes. Archives of Environmental Contamination and Toxicology , 1991, 21(1): 91-101 doi: 10.1007/BF01055562 pmid:1654867
|
19 |
Qiao M, Chen Y, Zhang Q, Huang S, Ma M, Wang C, Wang Z. Identification of Ah receptor agonists in sediment of Meiliang Bay, Taihu Lake, China. Environmental Science & Technology , 2006, 40(5): 1415-1419 doi: 10.1021/es051456p pmid:16568750
|
20 |
Shen C, Huang S, Wang Z, Qiao M, Tang X, Yu C, Shi D, Zhu Y, Shi J, Chen X, Setty K, Chen Y. Identification of ah receptor agonists in soil of E-waste recycling sites from Taizhou area in China. Environmental Science & Technology , 2008, 42(1): 49-55 doi: 10.1021/es071162z pmid:18350874
|
21 |
Gaido K W, Leonard L S, Lovell S, Gould J C, Baba? D, Portier C J, McDonnell D P. Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay. Toxicology and Applied Pharmacology , 1997, 143(1): 205-212 doi: 10.1006/taap.1996.8069 pmid:9073609
|
22 |
Li J, Ma M, Wang Z J. A two-hybrid yeast assay to quantify the effects of xenobiotics on thyroid hormone-mediated gene expression. Environmental Toxicology and Chemistry/SETAC , 2008, 27(1): 159-167 doi: 10.1897/07-054.1 pmid:18092857
|
23 |
Wang J X, Xie P, Kettrup A, Schramm K W. Inhibition of progesterone receptor activity in recombinant yeast by soot from fossil fuel combustion emissions and air particulate materials. The Science of The Total Environment , 2005, 349(1-3): 120-128 doi: 10.1016/j.scitotenv.2005.01.019 pmid:16198674
|
24 |
Donato M T, Gómez-Lechón M J, Castell J V. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Analytical Biochemistry , 1993, 213(1): 29-33 doi: 10.1006/abio.1993.1381 pmid:8238878
|
25 |
Wang C, Wang Y, Kiefer F, Yediler A, Wang Z, Kettrup A. Ecotoxicological and chemical characterization of selected treatment process effluents of municipal sewage treatment plant. Ecotoxicology and Environmental Safety , 2003, 56(2): 211-217 doi: 10.1016/S0147-6513(02)00121-5 pmid:12927551
|
26 |
Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry , 1976, 72(1-2): 248-254 doi: 10.1016/0003-2697(76)90527-3 pmid:942051
|
27 |
Wu Z W, Wang X J, Zhao F G, You L. The Emission Soot of Biomass Fuels Combustion as a Source of Endocrine Disrupters. Philadelphia, PA, ETATS-UNIS: Taylor&amp, Francis, 2002
|
28 |
Cargou?t M, Perdiz D, Mouatassim-Souali A, Tamisier-Karolak S, Levi Y. Assessment of river contamination by estrogenic compounds in Paris area (France). The Science of The Total Environment , 2004, 324(1-3): 55-66 doi: 10.1016/j.scitotenv.2003.10.035 pmid:15081696
|
29 |
Svenson A, Allard A S, Ek M. Removal of estrogenicity in Swedish municipal sewage treatment plants. Water Research , 2003, 37(18): 4433-4443 doi: 10.1016/S0043-1354(03)00395-6 pmid:14511714
|
30 |
Fawell J K, Sheahan D, James H A, Hurst M, Scott S. Oestrogens and oestrogenic activity in raw and treated water in Severn Trent Water. Water Research , 2001, 35(5): 1240-1244 doi: 10.1016/S0043-1354(00)00367-5 pmid:11268844
|
31 |
Desbrow C, Routledge E J, Brighty G C, Sumpter J P, Waldock M. Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening. Environmental Science & Technology , 1998, 32(11): 1549-1558 doi: 10.1021/es9707973
|
32 |
Ying G G, Kookana R S, Ru Y J. Occurrence and fate of hormone steroids in the environment. Environment International , 2002, 28(6): 545-551 doi: 10.1016/S0160-4120(02)00075-2 pmid:12503920
|
33 |
Jobling S, Nolan M, Tyler C R, Brighty G, Sumpter J P. Widespread sexual disruption in wild fish. Environmental Science & Technology , 1998, 32(17): 2498-2506 doi: 10.1021/es9710870
|
34 |
Conroy O, Sáez A E, Quanrud D, Ela W, Arnold R G. Changes in estrogen/anti-estrogen activities in ponded secondary effluent. The Science of the total environment , 2007, 382(2-3): 311-323 doi: 10.1016/j.scitotenv.2007.04.033 pmid:17543371
|
35 |
van der Oost R, Beyer J, Vermeulen N P E. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology , 2003, 13(2): 57-149 doi: 10.1016/S1382-6689(02)00126-6 pmid:21782649
|
36 |
Vega-López A, Ramón-Gallegos E, Galar-Martínez M, Jiménez-Orozco F A, García-Latorre E, Domínguez-Lípez M L. Estrogenic, anti-estrogenic and cytotoxic effects elicited by water from the type localities of the endangered goodeid fish Girardinichthys viviparus. Comparative Biochemistry and Physiology-Part C: Toxicology & Pharmacology , 2007, 145(3): 394-403 doi: 10.1016/j.cbpc.2007.01.008 pmid:17317326
|
37 |
Sohoni P, Sumpter J P. Several environmental oestrogens are also anti-androgens. The Journal of endocrinology , 1998, 158(3): 327-339 doi: 10.1677/joe.0.1580327 pmid:9846162
|
38 |
Xu L C, Sun H, Chen J F, Bian Q, Qian J, Song L, Wang X R. Evaluation of androgen receptor transcriptional activities of bisphenol A, octylphenol and nonylphenol in vitro. Toxicology , 2005, 216(2-3): 197-203 doi: 10.1016/j.tox.2005.08.006 pmid:16169144
|
39 |
Kiparissis Y, Metcalfe T L, Balch G C, Metcalfe C D. Effects of the antiandrogens, vinclozolin and cyproterone acetate on gonadal development in the Japanese medaka (Oryzias latipes). Aquatic Toxicology (Amsterdam, Netherlands) , 2003, 63(4): 391-403 doi: 10.1016/S0166-445X(02)00189-3 pmid:12758004
|
40 |
Tollefsen K E, Harman C, Smith A, Thomas K V. Estrogen receptor (ER) agonists and androgen receptor (AR) antagonists in effluents from Norwegian North Sea oil production platforms. Marine Pollution Bulletin , 2007, 54(3): 277-283 doi: 10.1016/j.marpolbul.2006.07.012 pmid:17258235
|
41 |
Graham J D, Clarke C L. Physiological action of progesterone in target tissues. Endocrine Reviews , 1997, 18(4): 502-519 doi: 10.1210/er.18.4.502 pmid:9267762
|
42 |
Vonier P M, Crain D A, McLachlan J A, Guillette L J Jr, Arnold S F. Interaction of environmental chemicals with the estrogen and progesterone receptors from the oviduct of the American alligator. Environmental Health Perspectives , 1996, 104(12): 1318-1322 doi: 10.1289/ehp.961041318 pmid:9118873
|
43 |
Pickford D B, Morris I D. Effects of endocrine-disrupting contaminants on amphibian oogenesis: methoxychlor inhibits progesterone-induced maturation of Xenopus laevis oocytes in vitro. Environmental Health Perspectives , 1999, 107(4): 285-292 doi: 10.1289/ehp.99107285 pmid:10090707
|
44 |
Klotz D M, Ladlie B L, Vonier P M, McLachlan J A, Arnold S F. o,p’-DDT and its metabolites inhibit progesterone-dependent responses in yeast and human cells. Molecular and Cellular Endocrinology , 1997, 129(1): 63-71 doi: 10.1016/S0303-7207(96)04041-5 pmid:9175630
|
45 |
Wen D X, Xu Y F, Mais D E, Goldman M E, McDonnell D P. The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells. Molecular and Cellular Biology , 1994, 14(12): 8356-8364 pmid:7969170
|
46 |
Horard B, Vanacker J M. Estrogen receptor-related receptors: orphan receptors desperately seeking a ligand. Journal of Molecular Endocrinology , 2003, 31(3): 349-357 doi: 10.1677/jme.0.0310349 pmid:14664699
|
47 |
Takayanagi S, Tokunaga T, Liu X, Okada H, Matsushima A, Shimohigashi Y. Endocrine disruptor bisphenol A strongly binds to human estrogen-related receptor gamma (ERRgamma) with high constitutive activity. Toxicology Letters , 2006, 167(2): 95-105 doi: 10.1016/j.toxlet.2006.08.012 pmid:17049190
|
48 |
Greschik H, Flaig R, Renaud J P, Moras D. Structural basis for the deactivation of the estrogen-related receptor gamma by diethylstilbestrol or 4-hydroxytamoxifen and determinants of selectivity. The Journal of Biological Chemistry, 2004, 279(32): 33639-33646 doi: 10.1074/jbc.M402195200 pmid:15161930
|
49 |
Tremblay G B, Bergeron D, Giguere V. 4-Hydroxytamoxifen is an isoform-specific inhibitor of orphan estrogen-receptor-related (ERR) nuclear receptors beta and gamma. Endocrinology , 2001, 142(10): 4572-4575 doi: 10.1210/en.142.10.4572 pmid:11564725
|
50 |
Ariazi E A, Clark G M, Mertz J E. Estrogen-related receptor alpha and estrogen-related receptor gamma associate with unfavorable and favorable biomarkers, respectively, in human breast cancer. Cancer Research , 2002, 62(22): 6510-6518 pmid:12438245
|
51 |
Aoki Y. Polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, and polychlorinated dibenzofurans as endocrine disrupters—what we have learned from Yusho disease. Environmental Research , 2001, 86(1): 2-11 doi: 10.1006/enrs.2001.4244 pmid:11386736
|
52 |
Díaz-Ferrero J, Rodríguez-Larena M C, Comellas L, Jiménez B.Bioanalytical methods applied to endocrine disrupting polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans: a review. TrAC Trends in Analytical Chemistry , 16(10): 563-573
|
53 |
Balaguer P, Joyeux A, Denison M S, Vincent R, Gillesby B E, Zacharewski T. Assessing the estrogenic and dioxin-like activities of chemicals and complex mixtures using in vitro recombinant receptor-reporter gene assays. Canadian Journal of Physiology and Pharmacology , 1996, 74(2): 216-222 pmid:8723035
|
54 |
Louiz I, Kinani S, Gouze M E, Ben-Attia M, Menif D, Bouchonnet S, Porcher J M, Ben-Hassine O K, A?t-A?ssa S. Monitoring of dioxin-like, estrogenic and anti-androgenic activities in sediments of the Bizerta lagoon (Tunisia) by means of in vitro cell-based bioassays: contribution of low concentrations of polynuclear aromatic hydrocarbons (PAHs). The Science of The Total Environment , 2008, 402(2-3): 318-329 doi: 10.1016/j.scitotenv.2008.05.005 pmid:18550148
|
55 |
Reungoat J, Macova M, Escher B I, Carswell S, Mueller J F, Keller J. Removal of micropollutants and reduction of biological activity in a full scale reclamation plant using ozonation and activated carbon filtration. Water Research , 2010, 44(2): 625-637 doi: 10.1016/j.watres.2009.09.048 pmid:19863988
|
56 |
Macova M, Escher B I, Reungoat J, Carswell S, Chue K L, Keller J, Mueller J F. Monitoring the biological activity of micropollutants during advanced wastewater treatment with ozonation and activated carbon filtration. Water Research , 2010, 44(2): 477-492 doi: 10.1016/j.watres.2009.09.025 pmid:19854465
|
57 |
Blanchard M, Teil M J, Ollivon D, Legenti L, Chevreuil M. Polycyclic aromatic hydrocarbons and polychlorobiphenyls in wastewaters and sewage sludges from the Paris area (France). Environmental Research , 2004, 95(2): 184-197 doi: 10.1016/j.envres.2003.07.003 pmid:15147924
|
58 |
Busetti F, Heitz A, Cuomo M, Badoer S, Traverso P. Determination of sixteen polycyclic aromatic hydrocarbons in aqueous and solid samples from an Italian wastewater treatment plant. Journal of Chromatography. A , 2006, 1102(1-2): 104-115 doi: 10.1016/j.chroma.2005.10.013 pmid:16256127
|
59 |
Smirnov A D, Schecter A, P?pke O, Beljak A A. Conclusions from Ufa, Russia, drinking water dioxin cleanup experiments involving different treatment technologies. Chemosphere , 1996, 32(3): 479-489 doi: 10.1016/0045-6535(95)00235-9 pmid:8907225
|
60 |
Ternes T A, Meisenheimer M, McDowell D, Sacher F, Brauch H J, Haist-Gulde B, Preuss G, Wilme U, Zulei-Seibert N. Removal of pharmaceuticals during drinking water treatment. Environmental Science & Technology , 2002, 36(17): 3855-3863 doi: 10.1021/es015757k pmid:12322761
|
61 |
Nelson J, Bishay F, van Roodselaar A, Ikonomou M, Law F C. The use of in vitro bioassays to quantify endocrine disrupting chemicals in municipal wastewater treatment plant effluents. The Science of The Total Environment , 2007, 374(1): 80-90 doi: 10.1016/j.scitotenv.2006.11.031 pmid:17257656
|
62 |
Servos M R, Bennie D T, Burnison B K, Jurkovic A, McInnis R, Neheli T, Schnell A, Seto P, Smyth S A, Ternes T A. Distribution of estrogens, 17β-estradiol and estrone, in Canadian municipal wastewater treatment plants. The Science of The Total Environment , 2005, 336(1-3): 155-170 doi: 10.1016/j.scitotenv.2004.05.025 pmid:15589256
|
63 |
Kraus W L, Weis K E, Katzenellenbogen B S. Inhibitory cross-talk between steroid hormone receptors: differential targeting of estrogen receptor in the repression of its transcriptional activity by agonist- and antagonist-occupied progestin receptors. Molecular and Cellular Biology , 1995, 15(4): 1847-1857 pmid:7891678
|
64 |
Ohtake F, Fujii-Kuriyama Y, Kawajiri K, Kato S. Cross-talk of dioxin and estrogen receptor signals through the ubiquitin system. The Journal of Steroid Biochemistry and Molecular Biology , 2011, 127(1-2): 102-107 doi: 10.1016/j.jsbmb.2011.03.007 pmid:21397018
|
65 |
Tran D Q, Klotz D M, Ladlie B L, Ide C F, McLachlan J A, Arnold S F. Inhibition of progesterone receptor activity in yeast by synthetic chemicals. Biochemical and Biophysical Research Communications , 1996, 229(2): 518-523 doi: 10.1006/bbrc.1996.1836 pmid:8954930
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|