Improvement of plant cryosection

doi:10.1007/s11515-012-2127-9

Front Biol

2012, Vol. 7

Issue (4) : 374-377 https://doi.org/10.1007/s11515-012-2127-9

RESEARCH ARTICLE

Improvement of plant cryosection

Zhaopei ZHANG¹, Liyuan NIU¹, Xiling CHEN², Xu XU¹, Zhengang RU³(

)

1. Lab Center, Henan Institute of Science and Technology, Xinxiang 453003, China; 2. College of Resources and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China; 3. Wheat Improvement Center, Henan Institute of Science and Technology, Xinxiang 453003, China

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Abstract

Cryosection in plants is usually challenging because of the larger amounts of water contained in plant cell than animal cell. The formation of ice crystals within the plant cells easily destroys subcellular detail during freeze processing. And cell walls make freezing plant tissues hardly sliced and preserve the cell structure in subtle ways. In this study, the technique was improved on infusion, embedding and flatting sections with the winter wheat Variety Aikang58 as raw material.

Keywords cryosection wheat histochemistry

Corresponding Author(s): RU Zhengang,Email:ruzhengang@yahoo.cn

Issue Date: 01 August 2012

Cite this article:

Zhaopei ZHANG,Xiling CHEN,Xu XU, et al. Improvement of plant cryosection[J]. Front Biol, 2012, 7(4): 374-377.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-012-2127-9
https://academic.hep.com.cn/fib/EN/Y2012/V7/I4/374

Tab.1 Glycerol concentration selection chart

Fig.1 Parts of leaf pieces in (transverse section). A: Smoothly sectioned wheat leaf is show (no staining). Palisade cell (PC), spongy cell (SC) and epidermal cell (white arrows) are clearly seen. × 400; B: leaf vein is indicated by a black arrowhead. × 65; C: chloroplasts (Ch) and a pair of guard cell (black arrowhead) are observed in lower epidermis of wheat leaf. × 1200.

Fig.2 Main steps in the protocol for the plant tissues cryosection improved

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